Abstract
A spectrophotometric method of determining extractable lipids was compared with a gravimetric method to estimate intracellular polar and non-polar lipids in the oleaginous yeast, Candida curvata. The methods correlated well but the spectrophotometric method was simpler, required less sample, and its accuracy was not affected by non-lipid components.
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References
Evans, C.T. & Ratledge, C. 1983 A comparison of the oleaginous yeast, Candida curvata grown on different carbon sources in continuous and batch culture. Lipids 18, 623–629.
Folch, J., Lees, M. & Sloane-Stanley, G.H. 1957 A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.
Moreton, R.S. 1989 Yeast lipid estimation by enzymatic and nuclear magnetic resonance methods. Applied and Environmental Microbiology 55, 3009–3011.
Nair, N.G., Song, C.H., Jiang, J.Y., Vine, J.H., Tattum, B. & Cho, K.Y. 1989 Lipid profile of Pleurotus sajor caju. Annals of Applied Biology 114, 167–176.
Renkonen, O. 1961 A note on spectrophotometric determination of acyl ester groups in lipids. Biochimica et Biophysica Acta 54, 361–362.
Synder, F. & Stephens, N. 1959 A simplified spectrophotometric determination of ester groups in liquids. Biochimica et Biophysica Acta 34, 244–245.
Additional information
L.W. Wang, D. Radford and K.Y. Cho are with the Microbiology Department, University of Sydney, NSW 2006, Australia. N.G. Nair is with NSW Agriculture, Rydalmere, NSW 2116, Australia.
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Wang, L.W., Radford, D., Cho, K.Y. et al. Spectrophotometric determination of polar and non-polar lipids in oleaginous yeast. World J Microbiol Biotechnol 9, 350–352 (1993). https://doi.org/10.1007/BF00383078
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DOI: https://doi.org/10.1007/BF00383078