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Resolution ofFusarium sporotrichioides proteins by two-dimensional polyacrylamide gel electrophoresis and identification by sequence homology comparison in protein data base

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Journal of Biomedical Science

Abstract

Proteins fromFusarium sporotrichioides M-1-1, a T2-toxin-producing strain, were separated by two-dimensional polyacrylamide gel electrophoresis. One thousand two hundred and forty-four protein spots were resolved and 103 protein spots were subjected to N-terminal sequencing. Fifty-eight protein spots were sequenced and 48 proteins were observed to have blocked N termini. Forty out of 58 sequenced proteins were identified by homology search against the PIR protein sequence data base and protein superfamily data base, while the residual 18 sequences were not identified. Twenty-seven of the N-terminal-blocked proteins were subjected to mild anhydrous hydrazine vapor deblocking. Twenty-four spots were not deblocked indicating the presence of acyl groups at the N termini, while 3 proteins were deblocked showing the blocked group to be pyrroglutamyl carboxylic acid residues. The results can provide a more global view of cellular genetic expression than any other technique. The created data may offer a unique opportunity to link information with DNA sequence data.

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Authors and Affiliations

  1. Department of Applied Biological Science, Science University of Tokyo, Noda

    Norifumi Miyatake

  2. Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Tokyo

    Lu-Ping Chow, Yoshitsugu Sugiura & Yoshio Ueno

  3. Department of Microbiology I, School of Veterinary Medicine, Azabu University, Sagamihara, Japan

    Nobuhito Fukaya & Kiyoshi Tabuchi

  4. Research Institute for Biosciences Science, University of Tokyo, 2669 Yamazaki, 278, Noda, Japan

    Lu-Ping Chow, Norifumi Miyatake, Katsuhisa Horimoto, Yoshio Ueno & Akira Tsugita

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  1. Lu-Ping Chow
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  2. Nobuhito Fukaya
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  4. Katsuhisa Horimoto
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  5. Yoshitsugu Sugiura
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  6. Kiyoshi Tabuchi
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  7. Yoshio Ueno
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  8. Akira Tsugita
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Chow, LP., Fukaya, N., Miyatake, N. et al. Resolution ofFusarium sporotrichioides proteins by two-dimensional polyacrylamide gel electrophoresis and identification by sequence homology comparison in protein data base. J Biomed Sci 2, 343–352 (1995). https://doi.org/10.1007/BF02255221

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  • DOI: https://doi.org/10.1007/BF02255221

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