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Determination of acetylcholinesterase activity by gas-liquid chromatography

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Bulletin of Experimental Biology and Medicine Aims and scope

Conclusions

  1. 1.

    A modified Deans' method for quantitative gas-chromatographic determination of trace components in a large volume of sample of test mixture introduced into the apparatus was developed and used.

  2. 2.

    In the suggested modification this method can be used for gas-chromatographic determination of microquantities of acetic acid.

  3. 3.

    Deans' method can be used for quantitative differential determination of acetylcholinesterase and pseudocholinesterase in the blood, based on determination of end products of hydrolysis of specific substrates.

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Literature Cited

  1. Z. M. Murav'eva, Vopr. Med. Khimii,7, No. 1, 97 (1961).

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  2. B. V. Stolyarov, I. M. Savinov, and A. G. Vitenberg, Textbook of Practical Exercises in Gas Chromatography [in Russian], Khimiya, Leningrad (1978).

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  3. D. R. Deans, Chromatographia, 18 (1968).

  4. D. R. Deans, Analyt. Chem.,43, No. 14, 2026 (1971).

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Authors
  1. A. D. Ado
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  2. R. M. Zolotareva
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  3. B. M. Zolotarev
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Additional information

Research Group for the Study of Bronchial Asthma, N. I. Pirogov Second Moscow Medical Institute. Department of Physical Chemistry, Timiryazev Agricultrual Academy, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 102, No. 9, pp. 377–379, September, 1986.

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Ado, A.D., Zolotareva, R.M. & Zolotarev, B.M. Determination of acetylcholinesterase activity by gas-liquid chromatography. Bull Exp Biol Med 102, 1312–1313 (1986). https://doi.org/10.1007/BF00842265

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  • DOI: https://doi.org/10.1007/BF00842265

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