Abstract
It is shown that cultured cells of the CV-1 line possess the capacity for high-affinity binding of ceruloplasmin, show a kinetics of saturation, and internalize ceruloplasmin. Propagation of cells in medium supplemented with fetal calf serum depleted of ceruloplasmin results in a two-fold increase of high-affinity receptor expression on the cell surface. This phenomenon is not accompanied by any change in the receptor-ligand affinity. Ceruloplasmin binding to the cell surface and subsequent internalization do not lead to its marked degradation.
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V. S. Baranov, V. N. Gorbunova, L. V. Puchkova,et al., Ontogenez,17, 37–46 (1986).
L. V. Puchkova, T. D. Aleinikova, N. V. Tsymbalenko,et al., Biokhimiya,59, 296–303 (1994).
L. V. Puchkova, L. K. Sasina, T. D. Aleinikova, and V. S. Gaitskhoki,Byull. Eksp. Biol. Med.,117, No 1, 83–85 (1994).
G. Barnes and E. Frieden,Biochem. Biophys. Res. Commun.,125, 157–162 (1984).
E. Frieden and H. S. Hsieh,Adv. Enzymol.,14, 187–236 (1976).
V. Iyengar, G. J. Brewer, R. D. Dick, and C. Owyand,J. Lab. Clin. Med.,111, 267–274 (1988).
J. L. Jaeger, N. Shimizu, and J. D. Gitlin,Biochem. J.,280, 671–677 (1991).
U. K. Laemmli,Nature,227, 680–685 (1970).
M. K. Skinner and M. D. Griswold,Biol. Reprod.,29, 1225–1229 (1983).
K. Tomita, T. Yoshida, A. Yoshimura,et al., J. Biol. Chem.,262, 1398–1404 (1987).
I. A. Verbina, L. V. Puchkova, V. S. Gaitskhoki, and S. A. Ntifakh,FEBS Lett.,292, 105–109 (1992).
C. Vulpe, B. Levinson, S. Whitney,et al., Nature Genetics,3, 7–13 (1993).
Y. Yamaguchi, M. E. Heiny, and J. D. Gitlin,Biochem. Biophys. Res. Commun.,197, 271–277 (1993).
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, No 4, pp. 417–420, April, 1995
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Puchkova, L.V., Sasina, L.K., Aleinikova, T.D. et al. Interaction of ceruloplasmin with the plasma membrane receptors of CV-1 cells and its feedback regulation. Bull Exp Biol Med 119, 404–407 (1995). https://doi.org/10.1007/BF02445905
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DOI: https://doi.org/10.1007/BF02445905