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Expression of the human α-1-antitrypsin gene in transgenic rats

  • Genetics
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

A genetic-engineering construction is developed to provide expression of the full-size cDNA of human α-1-antitrypsin controlled by the promoter elements of the murine metallothionein-1 gene. Transgenic animals were obtained by microinjection of pAT-MT plasmid into fertilized rat oocytes. The genome of such animals contains the sequences of human α-1-antitrypsin-cDNA revealed by blot- and dot-hybridization. Integrated human α-1-antitrypsin-cDNA copies are found with a high frequency (9 out of 22 animals) in the second-generation offspring obtained after crossings of transgenic animals. A protein with antigenic specificity of human a-1-antitrypsin is found in the serum of transgenic rats by the immunoblotting method.

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Authors and Affiliations

  1. Department of Molecular Genetics, Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg

    N. V. Tsymbalenko, G. F. Golinskii & V. S. Gaitskhoki

Authors
  1. N. V. Tsymbalenko
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  2. G. F. Golinskii
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  3. V. S. Gaitskhoki
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, No 7, pp. 81–83, July, 1995

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Tsymbalenko, N.V., Golinskii, G.F. & Gaitskhoki, V.S. Expression of the human α-1-antitrypsin gene in transgenic rats. Bull Exp Biol Med 120, 727–729 (1995). https://doi.org/10.1007/BF02444673

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  • DOI: https://doi.org/10.1007/BF02444673

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