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A Quantitative Genetic Analysis of Locomotor Activity in CXB Recombinant Inbred Mice

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Abstract

Recent studies have identified genes that influence the length of the circadian period maintained by mice housed under constant lighting conditions. However, a less studied circadian activity variable is the amplitude of daily oscillations in locomotor activity. This parameter reflects spontaneous activity exhibited under standard lighting and housing conditions and, therefore, differs conceptually from assessments of exploratory or open-field activity, voluntary wheel-running, or circadian period during exposure to constant light or constant darkness conditions. We recently observed a greater daily amplitude of oscillation in spontaneous locomotor activity in C57BL/6 mice compared to BALB/cBy mice. To identify genetic loci with potential linkage to circadian variation in the amount of locomotor activity, we measured the spontaneous activity of 13 CXB recombinant inbred (RI) strains of mice. The probability density distributions of locomotor activity phenotypes for the 13 CXB RI strains were consistent with the presence of a low number of major quantitative trait loci affecting this trait. Regions of chromosomes 3, 8, 12, 13, and 19 showed provisional linkage to strain variation in locomotor activity. Probabilities of linkage were not sufficient for declaration of an activity-related quantitative trait locus but were sufficient to warrant further analysis either with additional RI strains or with F2 panels.

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REFERENCES

  • Belknap, J. K., Crabbe, J. C., Plomin, R., McClearn, G. E., Sampson, K. E., O'Toole, L. A., and Gora-Maslak, G. (1992). Single-locus control of saccharin intake in BXD/Ty recombinant inbred (RI) mice: Some methodological implications for RI strain analysis. Behav. Genet. 22:81–100.

    Google Scholar 

  • Churchill, G. A., and Doerge, R. W. (1994). Empirical threshold values for quantitative trait mapping. Genetics 138:963–971.

    Google Scholar 

  • Connolly, M. S., and Lynch, C. B. (1981). Circadian variation of strain differences in body temperature and activity in mice. Physiol. Behav. 27:1045–1049.

    Google Scholar 

  • Crabbe, J. C. (1986). Genetic differences in locomotor activation in mice. Pharmacol. Physiol. Behav. 25:289–292.

    Google Scholar 

  • DeFries, J. C., Hegmann, J. P., and Weir, M. W. (1966). Open-field behavior in mice: Evidence for a major gene effect mediated by the visual system. Science 154:1577–1579.

    Google Scholar 

  • DeFries, J. C., Gervais, M. C., and Thomas, E. A. (1978). Response to 30 generations of selection for open-field activity in laboratory mice. Behav. Genet. 8:3–13.

    Google Scholar 

  • Dietrich, W., Katz, H., Lincoln, S. E., Shin, H. S., Friedman, J., Dracopoli, N. C., and Lander, E. S. (1992). A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131:423–447.

    Google Scholar 

  • Flint, J., Corley, R., DeFries, J. C., Fulker, D. W., Gray, J. A., Miller, S., and Collins, A. C. (1995). A simple genetic basis for a complex psychological trait in laboratory mice. Science 269:1432–1435.

    Google Scholar 

  • Goldowitz, D., Wahlsten, D., and Wimer, R. E. (1992). Techniques for the Genetic Analysis of Brain and Behavior: Focus on the Mouse, Elsevier, New York.

    Google Scholar 

  • Haley, C. S., and Knott, S. A. (1992). A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69:315–324.

    Google Scholar 

  • Hegmann, J. P., and Possidente, B. (1981). Estimating genetic correlations from inbred strains. Behav. Genet. 11:103–114.

    Google Scholar 

  • Hofstetter, J. R., Mayeda, A. R., Possidente, B., and Nurnberger, J. I. (1995). Quantitative trait loci (QTL) for circadian rhythms of locomotor activity in mice. Behav. Genet. 25:545–556.

    Google Scholar 

  • Lander, E., and Kruglyak, L. (1995). Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results. Nature Genet. 11:241–247.

    Google Scholar 

  • Lassalle, J. M., Halley, H., and Roullet, P. (1994). Analysis of behavioral and hippocampal variation in congenic albino and pigmented BALB mice. Behav. Genet. 24:161–169.

    Google Scholar 

  • Logue, S. F., Owen, E. H., Rasmussen, D. L., and Wehner, J. M. (1997). Assessment of locomotor activity, acoustic and tactile startle, and prepulse inhibition of startle in inbred mouse strains and F1 hybrids: Implications of genetic background for single gene and quantitative trait loci analysis. Neuroscience 80:1075–1086.

    Google Scholar 

  • Lynch, M., and Walsh, B. (1998). Mapping and characterizing QTLs: Inbred line crosses. In Genetics and Analysis of Quantitative Characters, Sinauer, San Francisco

    Google Scholar 

  • Mayeda, A. R., Hofstetter, J. R., Belknap, J. K., and Nurnberger, J. I. (1996). Hypothetical quantitative trait loci (QTL) for circadian period of locomotor activity in CXB recombinant inbred strains of mice. Behav. Genet. 26:505–511.

    Google Scholar 

  • Neiderhiser, J. M., Plomin, R., and McClearn, G. E. (1992). The use of CXB recombinant inbred mice to detect quantitative trait loci in behavior. Physiol. Behav. 52:429–439.

    Google Scholar 

  • Oliverio, A., Eleftheriou, B. E., and Bailey, D. W. (1973). Exploratory activity: Genetic analysis of its modification by scopolamine and amphetamine. Physiol. Behav. 10:893–899.

    Google Scholar 

  • Peeler, D. F., and Nowakowski, R. S. (1987). Genetic factors and the measurement of exploratory activity. Behav. Neural Biol. 48:90–103.

    Google Scholar 

  • Plomin, R., McClearn, G. E., Gora-Masiak, G., and Neiderhiser, J. M. (1991). Use of recombinant inbred strains to detect quantitative trait loci associated with behavior. Behav. Genet. 21:99–116.

    Google Scholar 

  • Possidente, B., and Stephan, F. K. (1988). Circadian period in mice: analysis of genetic and maternal contributions to inbred strain differences. Behav. Genet. 18:109–117.

    Google Scholar 

  • Schwartz, W. J., and Zimmerman, P. (1990). Circadian timekeeping in BALB/c and C57BL/6 inbred mouse strains. J. Neurosci. 10:3685–3694.

    Google Scholar 

  • Sprott, R. L., and Staats, J. (1979). Behavioral studies using genetically defined mice—A bibliography (July 1976–August 1978). Behav. Genet. 9:87–102.

    Google Scholar 

  • Toth, L. A., Rehg, J. E., and Webster, R. G. (1995). Strain differences in sleep and other pathophysiological sequelae of influenza virus infection in naive and immunized mice. J. Neuroimmunol. 58:89–99.

    Google Scholar 

  • Vitaterna, M. H., King, D. P., Chang, A. M., Kornhauser, J. M., Lowrey, P. L., McDonald, J. D., Dove, W. F., Pinto, L. H., Turek, F. W., and Takahashi, J. S. (1994). Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior. Science 264:719–725.

    Google Scholar 

  • Williams, R. W. (1998). Neuroscience meets quantitative genetics: Using morphometric data to map genes that modulate CNS architecture. In Morrison, J. H., and Hof, P. R. (eds.), Society for Neuroscience Short Course on Ouantitative Neuroanatomy; Society for Neuroscience Education Committee, pp. 1–22.

  • Zeng, Z. B. (1994). Precision mapping of quantitative trait loci. Genetics 136:1457–1468.

    Google Scholar 

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Authors and Affiliations

  1. Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, 38105

    L. A. Toth

  2. Department of Anatomy and Neurobiology, University of Tennessee, Memphis, Tennessee, 38163

    R. W. Williams

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  1. L. A. Toth
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  2. R. W. Williams
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Correspondence to R. W. Williams.

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Toth, L.A., Williams, R.W. A Quantitative Genetic Analysis of Locomotor Activity in CXB Recombinant Inbred Mice. Behav Genet 29, 319–328 (1999). https://doi.org/10.1023/A:1021657800287

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  • DOI: https://doi.org/10.1023/A:1021657800287

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