Abstract
About 160 mutants of Schizosaccharomyces pombe with altered activity of the nonspecific acid phosphatase located on the cell surface were isolated. The following kinetic parameters were determined by automatic techniques on the mutants: (1) residual activity vs. wild type, (2) K m , (3) V max , (4) optimal pH, (5) percent pH curve. The ability of the various parameters to discriminate between genetic variants was investigated by applying to the results a suitable statistical quantitative index, R (resolving power), previously defined by us. The last two parameters had least resolving power, whereas the first three had about equal resolving power. This was found to be consistent with current concepts of enzyme and protein structure. These results could serve as guidelines in the choice of biochemical parameters for the study of polymorphisms in systems not previously investigated. Moreover, they provide information about the relative effects on the various parameters of mutations which give rise to the genetic variants and also about the molecular nature of mutations occurring at different loci involved in the control of acid phosphatase in S. pombe.
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Bauer, C., Cinci, A., Bronzetti, G. et al. Use of biochemical parameters for the individuation of genetic variants: Study of the relative efficiency of some parameters determined on a number of mutants of Schizosaccharomyces pombe with altered acid phosphatase activity. Biochem Genet 11, 1–15 (1974). https://doi.org/10.1007/BF00486614
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DOI: https://doi.org/10.1007/BF00486614