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In vitro iron attachment to the intestinal brush border

Effect of iron stores and other environmental factors

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Abstract

Iron attachment to brush border membrane of guinea pig small intestine was measured by an in vitro technic. Both inorganic and hemoglobin iron uptake was assessed under various conditions. Electron microscopic and biochemical studies showed that iron was adsorbed to the glycocalyx of the brush border membranes. Iron attachment was selective depending on the iron stores of the animal. Several iron-binding compounds reduced the uptake of inorganic iron when they were added at the beginning of the incubation period and did not affect attachment of hemoglobin iron. Gastric juice markedly increased attachment of heme iron to the pellet. This effect appeared to be related to the effect of acid on the hemoglobin molecule.

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References

  1. McDougal DB, Little KD, Crane RK: Studies on the mechanism of intestinal absorption of sugars: IV. Localization of galactose concentrations within the intestinal wall during active transport, in vitro. Biochim Biophys Acta 45:483–489, 1960

    Google Scholar 

  2. Donaldson RM, Mackenzie IL, Trier JS: Intrinsic factor-mediated attachment of vitamin B12 to brush borders and micro-villous membranes of hamster intestine. J Clin Invest 46:1215–1228, 1967

    Google Scholar 

  3. Miller D, Crane RK: The digestive function of the epithelium of the small intestine: II. Localization of disaccharide hydrolysis in the isolated brush border portion of intestinal epithelial cells. Biochim Biophys Acta 52:293–298, 1961

    Google Scholar 

  4. Greenberger NJ, Balcerzak SP, Ackerman GA: Iron uptake by isolated intestinal brush borders: changes induced by alterations in iron stores. J Lab Clin Med 73:711–721, 1969

    Google Scholar 

  5. Conrad ME, Weintraub LR, Sears DA, Crosby WH: Absorption of hemoglobin iron. Am J Physiol 211:1123–1230, 1966

    Google Scholar 

  6. Miller D, Crane RK: A procedure for the isolation of the epithelial brush border membrane of hamster small intestine. Anal Biochem 2:284–286, 1961

    Google Scholar 

  7. Lowry, OH, Rosebrough NH, Farr AL, Randall RJ: Protein measurement with the folin phenol reagent. J Lab Clin Med 193:265–275, 1951

    Google Scholar 

  8. Dahlquist A: Method for assay of intestinal dissaccharidases Anal Biochem 7:18–25, 1964

    Google Scholar 

  9. Mukherjee TM, Wynn-Williams A: A comparative study of the ultrastructure of microvilli in the epithelium of small and large intestine of mice. J Cell Biol 34:447–461, 1967

    Google Scholar 

  10. Luft JH: Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414, 1961

    Google Scholar 

  11. Conrad ME, Schade SG: Ascorbic acid chelates in iron absorption: a role for hydrochloric acid and bile. Gastroenterology 55:35–45, 1968

    Google Scholar 

  12. Kimber C, Patterson JF, Weintraub LR: Pathogenesis of iron deficiency anemia following partial gastrectomy: study of iron balance. JAMA 203:935–938, 1967

    Google Scholar 

  13. Wilcoxon F: Probability tables for individual comparisons by ranking methods. Biometrics 3:119–122, 1947

    Google Scholar 

  14. Rudzki Z, Deller DJ: Isolation and partial characterisation of the iron binding component of human gastric juice (Abstr) Proc Aust Biochem Soc 151:76, 1969

    Google Scholar 

  15. Wynter CVA, Williams R: Iron-binding properties of gastric juice in idiopathic haemochromatosis. Lancet 2:534–537, 1968

    Google Scholar 

  16. Powell LW, Wilson E: In vivo intestinal mucosal uptake of iron, body iron absorption and gastric juice iron binding in idiopathic haemochromatosis. Aust Ann Med 19:226–231, 1970

    Google Scholar 

  17. Kay AW: Effect of large doses of histamine on gastric secretion of hydrochloric acid: an augmented histamine test. Br Med J 2:77–80, 1953

    Google Scholar 

  18. Labbe RD, Nishida G: A new method of hemin isolation. Biochim Biophys Acta 26:437, 1957

    Google Scholar 

  19. Benedetti EL, Emmelot P: Studies on plasma membranes: IV. The ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J Cell Sci 2:499–512, 1967

    Google Scholar 

  20. Mukherjee TM, Staehelin LA: The fine structural organization of the brush border of the intestinal epithelial cells. (In press)

  21. Hahn PF, Bale WF, Ross JF, Balfour WM, Whipple GH: Radioactive iron absorption by the gastrointestinal tract: influence of anemia, anoxia and antecedent feeding; distribution in growing dogs. J Exp Med 78:169–188, 1943

    Google Scholar 

  22. Pirzio-Biroli G, Finch CA: Iron absorption: III. The influence of iron stores on iron absorption in the normal subject. J Lab Clin Med 55:216–220, 1960

    Google Scholar 

  23. Conrad ME, Crosby WH: Intestinal mucosal mechanisms controlling iron absorption. Blood 22:406–415, 1963

    Google Scholar 

  24. Weintraub LR, Conrad ME, Crosby WH: The significance of iron turnover in the control of iron absorption. Blood 24:19–24, 1964

    Google Scholar 

  25. Manis JG, Schachter D: Active transport of iron by intestine: features of the two-step mechanism. Am J Physiol 203:73–80, 1962

    Google Scholar 

  26. Bannerman RM: Quantitative aspects of hemoglobin-iron absorption. J Lab Clin Med 65:944–950, 1965

    Google Scholar 

  27. Bothwell TH, Pirzio-Biroli G, Finch CA: Iron absorption: I. Factors influencing absorption. J Lab Clin Med 51:24–36, 1958

    Google Scholar 

  28. Conrad ME, Weintraub LR, Crosby WH: The role of the intestine in iron kinetics. J Clin Invest 43:963–974, 1964

    Google Scholar 

  29. Charlton RW, Jacobs P, Torrance JD, Bothwell TH: The role of the intestinal mucosa in iron absorption. J Clin Invest 44:543–554, 1965

    Google Scholar 

  30. Callender ST: The intestinal mucosa and iron absorption. Br Med Bull 23:263–265, 1967

    Google Scholar 

  31. Hartman RS, Conrad ME, Hartman RE, Joy RTT, Crosby L: Ferritin-containing bodies in human small intestinal epithelium. Blood 22:397–405, 1963

    Google Scholar 

  32. Bennett C: Migration of glycoprotein from Golgi apparatus to cell coat in the columnar cells of the duodenal epithelium. J Cell Biol 45:668–673, 1970

    Google Scholar 

  33. Bennett G, Leblond CP: Formation of cell coat material for the whole surface of columnar cells in the rat small intestine as visualized by radioautography with L-fucose-3H. J Cell Biol 46:409–416, 1970

    Google Scholar 

  34. Hwang YF, Brown EB: Effect of desferrioxamine on iron absorption. Lancet 1:135–137, 1965

    Google Scholar 

  35. Callender ST, Mallet BJ, Smith MD: Absorption of haemoglobin iron. Br J Haematol 3:186–192, 1957

    Google Scholar 

  36. Turnbull A, Cleton F, Finch CA: Iron absorption: IV. The absorption of hemoglobin iron. J Clin Invest 41:1897–1907, 1962

    Google Scholar 

  37. Aitken MA, Spray GH, Walters G: Gastric pepsin and the excretion of uropepsinogen in anaemia. Clin Sci 13:119–126, 1954

    Google Scholar 

  38. Conrad ME, Cortell S, Williams HL, Foy AL: Polymerization and intraluminal factors in the absorption of hemoglobin iron. J Lab Clin Med 68:659–668, 1966

    Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, University of Adelaide, 5001, Adelaide, South Australia

    Carlien L. Kimber MD, MRACP, T. Mukherjee MD, DGO & D. J. Deller MD, PhD, MRCP(Lond), FRACP

  2. Institute of Medical and Veterinary Science, 5001, Adelaide, South Australia

    Carlien L. Kimber MD, MRACP, T. Mukherjee MD, DGO & D. J. Deller MD, PhD, MRCP(Lond), FRACP

Authors
  1. Carlien L. Kimber MD, MRACP
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  2. T. Mukherjee MD, DGO
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  3. D. J. Deller MD, PhD, MRCP(Lond), FRACP
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Supported in part by a Roche research grant.

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Kimber, C.L., Mukherjee, T. & Deller, D.J. In vitro iron attachment to the intestinal brush border. Digest Dis Sci 18, 781–791 (1973). https://doi.org/10.1007/BF01070848

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  • DOI: https://doi.org/10.1007/BF01070848

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