Abstract
Absorption of carbohydrate was quantitated in 49 subjects without disease of the small bowel using a new technique for ileal perfusion. A double-lumen tube with an attached balloon was inserted retrograde through the colon and used to quantify arrival in the ileum ofD-xylose and a nonabsorbable marker which had been taken orally. In the same way, absorption of sucrose and the effects of an inhibitor of alpha-glucosidase were also studied. Insertion of the assembly through the colon and intubation of the terminal ileum was usually possible within 30 min; we have designated the technique, endoscopic retrograde bowel insertion (ERBI). The test meals were 500 ml of water containing either 25 gD-xylose and 5 g polyethylene glycol (PEG 4000), or 100 g sucrose with 5 g PEG. Sucrose meals also contained 0, 100, or 200 mg of an inhibitor of alpha-glucosidase (BAYg5421). At the end of a 5-hr test period, the ratio of recovery ofD-xylose relative to that of PEG indicated that 69% ofD-xylose was absorbed. Five-hour urinary excretion ofD-xylose was 31% of that ingested, or 45% of theD-xylose which was absorbed. Sucrose was recovered in ileal samples only when administered together with inhibitor. Rates of sucrose absorption with BA Y g5421, 100 and 200 mg, were 75% and 65%, respectively. The perfusion technique of ERBI is a rapid and reproduceable approach to the distal small intestine of man which could be of value in the investigation of intestinal absorption.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Van de Kamer JH, Huinink HB, Weyers HA: Rapid method for the determination of fat in feces. J Biol Chem 177:347–355, 1949
Bond JH, Levitt MD: Fate of soluble carbohydrate in the colon of rats and man. J Clin Invest 57:1158–1164, 1976
Newcomer AD, McGill DB: Lactose tolerance tests in adults with normal lactase activity. Gastroenterology 50:340–346, 1966
Modigliani R, Ramband JC, Bernier JJ: The method of intraluminal perfusion of the human small intestine. I. Principle and technique. Digestion 9:176–192, 1973
Debongnie JC, Newcomer AD, McGill DB, Phillips SF: Absorption of nutrients in lactase deficiency. Dig Dis Sci 24:225–231, 1979
Malawer SJ, Powell DW: An improved turbidimetric analysis of polyethylene glycol utilizing an emulsifier. Gastroenterology 53:250–256, 1976
Roe JH, Rice EW: A photometric method for the determination of free pentoses in animal tissues. J Biol Chem 173:507–512, 1948
Kerstell J: A simplified method for the determination of xylose in urine. Scand J Clin Invest 13:637–641, 1961
Dahlqvist A, Borgström B: Digestion and absorption of disaccharides in man. Biochem J 81:411–418, 1961
Phillips SF, Giller J: The contribution of the colon to electrolyte and water conservation in man. J Lab Clin Med 81:733–746, 1973
Morris AI, Turnberg LA, Hall L, Pimblett K: The influence of a parasympathetic agonist and antagonist on human intestinal transportin vivo. Gastroenterology 79:861–866, 1980
Sasaki D, Munakata A, Saitoh Y, Yoshida Y: Antispasmodic effect of prifinium bromide on the proximal and distal colon in patients with diverticular disease. Gastroenterol Jpn 16:344–349, 1981
Chauve A, Devroede G, Bastin E: Intraluminal pressure during perfusion of the human colonin situ. Gastroenterology 70:336–340, 1976
Gallagher ND, Harrison DD, Skyring AP: Fluid and electrolyte disturbances in patients with long-established ileostomy. Gut 3:219–223, 1962
Kanaghinis T, Lubran M, Coghill NF: The composition of ileostomy fluid. Gut 4:322–338, 1963
Chung YC, Kim YS, Shadchehr A, Carrido A, Macgregor IL, Sleisenger MH: Protein digestion and absorption in human small intestine. Gastroenterology 76:1415–1421, 1979
Bond JH, Levitt MD: Investigation of small bowel transit time in man utilizing pulmonary hydrogen (H2) measurement. J Lab Clin Med 85:546–555, 1975
Bond JH, Currier B, Buchwald H, Levitt MD: Colonic conservation of malabsorbed carbohydrate. Gastroenterology 78:444–447, 1980
Benson JA, Culver PJ, Ragland S, Jones CM, Drummey GD, Bougas E: Thed-xylose absorption test in malabsorption syndromes. N Engl J Med 256:335–339, 1957
Wyngaarden JB, Segal S, Foley JB: Physiological disposition and metabolic fate of infused pentoses in man. J Clin Invest 36:1395–1407, 1957
Hiatt HH: Glycogen formation via the pentose phosphate pathway in micein vivo. J Biol Chem 224:851–859, 1957
Schmidt DD, Frommer W, Junge B, Müller L, Wingender W, Truscheit E: α-Glucosidase inhibitors, new complex oligosaccharides of microbial origin. Naturwissenschaften 64:S535, 1977
Puls W, Keup U, Kranse HP, Thomas G, Hoffmeister F: Glucosidase inhibition, a new approach to the treatment of diabetes, obesity, and hyperlipoproteinemia. Naturwissenschaften 64:S536, 1977
Caspary WF: Sucrose malabsorption in man after ingestion of α-glucoside hydrolase inhibitor. Lancet 10:1231–1233, 1978
Plus W, Keup U, Krause HP, Müller L, Schmidt DD, Thomas G, Truscheit E: Pharmacology of a glucosidase inhibitor. Front Horm Res 7:235–247, 1980
Stephen AM, Haddad AC, Phillips SF: Passage of carbohydrate into the colon. Gastroenterology 85:589–595, 1983
Maruhama Y, Nagasaki A, Kanazawa Y, Hirakawa H, Goto Y, Shimoyama T: Effects of glucoside-hydrolase inhibitor (BAYg/5421) on serum lipids lipoproteins and bile acids, fecal fat and bacterial flora, and intestinal gas production in hyperlipidemic patients. Tohoku J Exp Med 132:453–462, 1980
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Higuchi, S., Fukushi, G., Baba, T. et al. New method of testing for carbohydrate absorption in man. Digest Dis Sci 31, 369–375 (1986). https://doi.org/10.1007/BF01311671
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01311671