Skip to main content
SpringerLink
Log in

Autofluorescence in onset of gastric mucosal injury induced by hemorrhagic shock in rats

  • Original Article
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

In an attempt to clarify whether gastric mucosal autofluorescence can help us to recognize gastric lesions at the onset of their formation, we investigated the fluorescence generated from the gastric mucosa of rats under ischemia-reperfusion stress. Redcolored fluorescence appeared and began to increase within 5 min after reperfusion. Such an increase in fluorescence did not occur in the gastric mucosa under prolonged ischemia without reperfusion. The epifluorescence microscopy of mucosal cryosections revealed that fluorescence was present even when only superficial mucosal damage occurred. Spectrofluorometric and high-performance liquid chromatographic analysis of the fluorescent mucosa extract identified the fluorescent substances as porphyrins. These findings suggested that fluorescent porphyrins are generated in the mucosal layer during the introductory phase of mucosal lesion formation induced by ischemia-reperfusion stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Policard A: Etude sur les aspects offerts par des tumeurs expérimentales examinées à la lumière de Wood. C R Soc Biol 91:1423–1424, 1924

    Google Scholar 

  2. Georgerot H, Patte A: Fluorescence des épithéliomas à la lumière de Wood. Bull Soc Dermatol Syph 80:288–295, 1939

    Google Scholar 

  3. Figgie FHJ: Fluorescence studies on cancer; I. Porphyrin metabolism, Harderian gland fluorescence, and susceptibility to carcinogenic agents. Cancer Res 14:465–471, 1944

    Google Scholar 

  4. Ronchese F: The fluorescence of cancer under the Wood's light. Oral Surg Med Oral Pathol 7:967–971, 1954

    Google Scholar 

  5. Ghadially FN, Neish WJP: Red fluorescence of experimentally induced and human tumors. J Pathol Bacteriol 80:345–351, 1960

    PubMed  Google Scholar 

  6. Ghadially FN, Neish WJP: Porphyrin fluorescence of experimentally produced squamous cell carcinoma. Nature 188:1124, 1960

    Google Scholar 

  7. Ghadially FN, Neish WJP, Dawkins HC: Mechanisms involved in the production of red fluorescence of human and experimental tumors. J Pathol Bacteriol 85:77–92, 1963

    PubMed  Google Scholar 

  8. Alfano RR, Lam W, Zarrabi H, Alfano MA, Cordero J, Tata DB, Swenberg CE: Human teeth with and without caries studied by laser scattering fluorescence and absorption spectroscopy. IEEE Quantum Electron QE-20:1512–1516, 1984

    Google Scholar 

  9. Satori M, Sauerbrey R, Kubodera S, Tittel FK, Roberts R, Henry PD: Autofluorescence maps of atherosclerotic human arteries—a new technique in medical imaging. IEEE Quantum Electron QE-23:1794–1797, 1987

    Google Scholar 

  10. Leffell DJ, Stetz ML, Milstone LM, Deckelbaum LI:In vivo fluorescence of human skin. Arch Dermatol 124:1514–1518, 1988

    PubMed  Google Scholar 

  11. Kapadia CR, Cutruzzola FW, O'Brien KM, Stetz ML, Enriquez R, Deckelbaum LI: Laser-induced fluorescence spectroscopy of human colonic mucosa. Gastroenterology 99:150–157, 1990

    PubMed  Google Scholar 

  12. Alfano RR, Tata DB, Cordero J, Tomashefsky P, Longo FW, Alfano MA: Laser induced fluorescence spectroscopy from native cancerous and normal tissue. IEEE Quantum Electron QE-20:1507–1511, 1984

    Google Scholar 

  13. Alfano RR, Tang GC, Pradhan A, Lam W, Choy DSJ, Opher E: Fluorescence spectra from cancerous and normal human breast and lung tissues. IEEE Quantum Electron QE-23:1806–1811, 1987

    Google Scholar 

  14. Alfano RR, Tang GC, Pradhan A, Bleich M, Choy DSJ, Opher E: Steady state and time-resolved laser fluorescence from normal and tumor lung and breast tissues. J Tumor Mark Oncol 3:165–174, 1988

    Google Scholar 

  15. Itoh M, Guth PH: Role of oxygen-derived free radicals in hemorrhagic shock induced gastric lesions. Gastroenterology 88:1162–1167, 1985

    PubMed  Google Scholar 

  16. Itoh M, Paulsen G, Guth PH: Hemorrhagic shock and acid gastric injury in the rat. Gastroenterology 90:1103–1110, 1986

    PubMed  Google Scholar 

  17. Tsimoyinnis EC, Sarros CJ, Tsimoyiannis JC, Moutesidou K, Akalestos G, Kotoulas OB: Ranitidine and oxygen derived free radical scavengers in haemorrhagic shock induced gastric lesions. Gut 29:826–829, 1988

    PubMed  Google Scholar 

  18. Yasue N, Guth PH: Role of exogenous acid and reperfusion in hemorrhagic shock-induced gastric lesions in the rat. Gastroenterology 94:1135–1143, 1988

    PubMed  Google Scholar 

  19. Granger DN, Rutili G, McCord JM: Superoxide radicals in feline intestinal ischemia. Gastroenterology 81:22–29, 1981

    PubMed  Google Scholar 

  20. Parks DA, Bulkley GB, Granger DN, Hamilton SR, McCord JM: Ischemic injury in the cat small intestine; role of superoxide radicals. Gastroenterology 82:9–15, 1982

    PubMed  Google Scholar 

  21. Parks DA, Granger DN: Ischemia-induced microvascular changes: Role of xanthine oxidase and hydroxyl radicals. Am J Physiol 245:G285-G289, 1983

    PubMed  Google Scholar 

  22. Koningsberger JC, Marx JJM, van Hattum J: Free radicals in gastroenterology. A review. Scand J Gastroenterol 23(suppl 154):30–40, 1988

    Google Scholar 

  23. Halliwell B, Gutteridge JMC: Protection against oxidants in biological system: The superoxide theory of oxygen toxicity.In Free Radicals in Biology and Medicine, 2nd ed. London, Clarendon Press·Oxford, 1989, pp 86–187

    Google Scholar 

  24. Frydman RB, Frydman B, Valasinas A: Protoporphyrin: Synthesis and Biosynthesis of Its Metabolic Intermediates.In The Porphyrins, Biochemistry Part A, vol 6. D Dolphin (ed). New York, Academic Press, 1979, pp 1–124

    Google Scholar 

  25. David MH, Werkhaven J: Endogenous porphyrin fluorescence in tumors. Lasers Surg Med 7:467–472, 1987

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. From the Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, 1-1 Tennodai, Tsukuba Science City, 305, Ibaraki, Japan

    Hirofumi Matsui MD,  Susumu Yamagata Jr. MD, Ken-Ichi Hirano PhD, Mitsuru Ishikawa PhD, Hisayuki Fukutomi MD & Toshiaki Osuga MD

  2. Tsukuba Research Laboratory, Hamamatsu Photonics, 5-9-2 Tokodai, Tsukuba Science City, 300-26, Ibaraki, Japan

    Hirofumi Matsui MD &  Susumu Yamagata Jr. MD

Authors
  1. Hirofumi Matsui MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Susumu Yamagata Jr. MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ken-Ichi Hirano PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mitsuru Ishikawa PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hisayuki Fukutomi MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Toshiaki Osuga MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsui, H., Yamagata, S., Hirano, KI. et al. Autofluorescence in onset of gastric mucosal injury induced by hemorrhagic shock in rats. Digest Dis Sci 39, 116–123 (1994). https://doi.org/10.1007/BF02090070

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02090070

Key words

Search

Navigation