Abstract
We here ascertain whether tryptase (a serine endoprotease released by mast cells) and cathepsin D (CD, a lysosomal hydrolase that seems able to derange the extracellular matrix) play a part in peptic ulcer disease and whether they are linked toHelicobacter pylori (Hp) infection. We studied 13 controls, 25 patients with gastric ulcer, 47 with duodenal ulcer, and 11 with duodenitis. Tryptase and CD were measured in mucosal biopsies (body and antrum of the stomach and duodenum) using IRMA methods. Hp infection was histologically evaluated (Giemsa). Tryptase and CD levels were higher (25%) in patients with active peptic ulcer, whether gastric or duodenal. In Hp-positive patients the CD mucosal content was higher while tryptase mucosal levels were lower than in Hp-negative patients. Tryptase was correlated with gastrin content. CD seems to be mainly related to the phlogistic reaction of the mucosa to Hp infection; tryptase may reflect an indirect link between Hp infection, gastrin release, and the function of mast cells.
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Stechschulte DJ, Morris DC, Jilka RL, Stechschulte DJ, Dileepan KN: Impaired gastric acid secretion in mast cell-deficient mice. Am J Physiol 259:G41-G47, 1990
Schwartz LB: Monoclonal antibodies against human mast cell tryptase demonstrate shared antigenic sites on subunits of tryptase and selective localization of the enzyme to mast cells. J Immunol 134:526–531, 1985
Wasserman SI: Mast cell biology. J Allergy Clin Immunol 86:590–593, 1990
Schwartz LB: Tryptase, a mediator of human mast cells. J Allergy Clin Immunol 86:594–598, 1990
Axon ATR:Campylobacter pyloridis: What role in gastritis and peptic ulcer? Br Med J 293:772–773, 1986
Clearfield HR:Helicobacter pylori: Aggressor or innocent bystander? Med Clin North Am 75(4):815, 1991
Graham DY:Campylobacter pylori and peptic ulcer disease. Gastroenterology 96:615–625, 1989
Rauws EAJ, Langenberg W, Houthoff HJ, Zanen HC, Tytgat GNJ:Campylobacter pyloridis-associated chronic antral gastritis. Gastroenterology 94:33–40, 1988
Moss SF, Legon S, Bishop AE, Polak JM, Calam J: Effect ofHelicobacter pylori on gastric somatostatin in duodenal ulcer disease. Lancet 340:930–932, 1992
Chung S, Kawai K: Protease activities in gastric cancer tissues. Clin Chim Acta 189:205–210, 1990
Senior RM, Campbell EJ: Neutral proteinases from human inflammatory cells: A critical review of their role in extracellular matrix degradation. Clin Lab Med 3:645, 1984
Vasishta A, Baker PR, Hopwood D, Holley PM, Cuschieri A: Proteinase-like peptidase activities in malignant and nonmalignant gastric tissue. Br J Surg 72:386–388, 1985
Weiss SJ: Tissue destruction by neutrophils. N Engl J Med 320:365–376, 1989
Bradford MM: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254, 1976
Plebani M, Di Mario F, Battistel M, Basso D, Mantovani G, Giacomini A, Burlina A: Measurement of tryptase in endoscopic biopsies: Distribution and relationship with ulcer disease. Clin Chim Acta 206:107–114, 1992
Bechi P, Dei R, Di Bello MG, Masini E:Helicobacter pylori potentiates histamine release from serosal rat mast cellsin vitro. Dig Dis Sci 38:944–949, 1993
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Plebani, M., Basso, D., Rugge, M. et al. Influence ofHelicobacter pylori on tryptase and cathepsin D in peptic ulcer. Digest Dis Sci 40, 2473–2476 (1995). https://doi.org/10.1007/BF02063258
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DOI: https://doi.org/10.1007/BF02063258