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A Sensitive Docetaxel Assay in Plasma by Solid-Phase Extraction and High Performance Liquid Chromatography – UV Detection: Validation and Suitability in Phase I Clinical Trial Pharmacokinetics

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Abstract

We have developed a specific and sensitive method aiming atdocetaxel (Taxotere®) determination in plasma of treatedpatients. This involved solid-phase extraction of 1 ml of plasmaonto carboxylic acid (CBA) grafted silica cartridges followed byreversed-phase liquid chromatography with UV detection. The bestselectivity was obtained through the use of C18 Uptisphere® asstationary phase. The low limit of quantitation obtained (LOQ:5 ng/ml) allowed measurements of docetaxel up to 24 hours afterone-hour infusions with low dosages of drug (60 mg/m2). Themethod was applied successfully to monitor docetaxel plasma levelswithin two protocols associating fixed dosages of either methotrexate or gemcitabine with escalating doses of Taxotere®.

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Authors
  1. Claude Joseph Ardiet
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  2. Brigitte Tranchand
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  3. Sylvie Zanetta
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  4. Aline Guillot
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  5. Elisabeth Bernard
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  6. Martine Peguy
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  7. Paul Rebattu
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  8. Jean-Pierre Droz
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Joseph Ardiet, C., Tranchand, B., Zanetta, S. et al. A Sensitive Docetaxel Assay in Plasma by Solid-Phase Extraction and High Performance Liquid Chromatography – UV Detection: Validation and Suitability in Phase I Clinical Trial Pharmacokinetics. Invest New Drugs 17, 325–333 (1999). https://doi.org/10.1023/A:1006327302041

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