Abstract
Purpose: Our purpose was to investigate whether DNA flow cytometric quantification and DNA polymorphism analysis are useful for cytogenetic diagnosis in the case of a complete hydatidiform mole that coexists with a living fetus.
Methods: Flow cytometric analysis of the nuclear DNA content and polymerase chain reaction (PCR) amplification of the minisatellite locus with the MCT118 probe were performed on the tissues (fetus, placenta and mole) obtained at the initial evacuation.
Results: DNA histograms of placental, fetal, and molar tissues showed diploid peaks. PCR products demonstrated that the allele of the mole was homozygous and inherited solely from the husband and that the mole differed genetically from the fetus and the placenta.
Conclusions: These results suggested that DNA flow cytometry and DNA polymorphism analysis may be useful for the cytogenetic diagnosis of a complete hydatidiform mole and a coexisting fetus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Vejerslev LO: Clinical management and diagnostic possibilities in hydatidiform mole with coexistent fetus. Obstet Gynecol Surv 1991;46:577–588
Goto S, Yamada A, Ishizuka T, Tomoda Y: Development of post molar trophoblastic disease after partial molar pregnancy. Gynecol Oncol 1993;48:165–170
Kajii T, Ohama L: Androgenic origin of hydatidiform mole. Nature 1977;268:633–634
Szulman AE, Surti U: The syndromes of hydatidiform mole. I. Cytogenetic and morphologic correlations. Am J Obstet Gynecol 1978;131:665–671
Lage JM, Mark SD, Roberts DJ, Goldstein DP, Bernstein MR, Berkowitz RS: A flow cytometric study of 137 fresh hydropic placentas: Correlation between types of hydatidiform moles and nuclear DNA ploidy. Obstet Gynecol 1992;79:403–410
Fisher FA, Sheppard DM, Lawler S: Twin pregnancy with complete hydatidiform mole (46,XX) and fetus (46,XY): Genetic origin proved by analysis of chromosome polymorphisms. BMJ 1982;284:1218–1220
Jeffreys AJ, Wilson V, Thein SL: Hypervariable “minisatellite” regions in human DNA. Nature 1985;314:67–73
Kasai K, Nakamura Y, White R: Amplification of a variable number of tandem repeats (VNTR) locus (pMCT118) by the polymerase chain reaction (PCR) and its application to forensic science. J Forensic Sci 1990;35:1196–1200
Bristow RE, Shumway JB, Khouzami AN, Witter FR: Complete hydatidiform mole and surviving coexistent twin. Obstet Gynecol Surv 1996;51:705–709.
Osada H, Iitsuka Y, Matsui H, Sekiya S: A complete hydatidiform mole coexisting with a normal fetus was confirmed by variable number tandem repeat (VNTR) polymorphism analysis using polymerase chain reaction. Gynecol Oncol 1995;56:90–93
Harada I, Tsutsumi O, Takai Y, Iida T, Sakai M, Yoshikawa H, Taketani T: DNA polymorphism analysis of a case of complete hydatidiform mole coexisting with a fetus. Hum Reprod 1997;12:2563–2566
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hirose, M., Kimura, T., Mitsuno, N. et al. DNA Flow Cytometric Quantification and DNA Polymorphism Analysis in the Case of a Complete Mole with a Coexisting Fetus. J Assist Reprod Genet 16, 263–267 (1999). https://doi.org/10.1023/A:1020319629999
Issue Date:
DOI: https://doi.org/10.1023/A:1020319629999