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Effects of Controlled Ovarian Hyperstimulation on Oocyte Quality in Terms of the Incidence of Apoptotic Granulosa Cells

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Abstract

Purpose: The aim was to investigate which ovarian hyperstimulation protocol performed in the same patients causes development of oocytes of good quality.

Methods: Twenty normo-ovulatory women underwent three different controlled ovarian hyperstimulation protocols for in vitro fertilization–embryo transfer. Patients underwent follicle aspiration after administration of human chorionic gonadotropin (hCG). The total number of retrieved oocytes, the number of mature oocytes, and the rate of mature oocytes were examined. Recovered granulosa cells were stained with Hoechst 33258 and examined by fluorescence microscopy to estimate the incidence of apoptotic cells.

Results: The total number of oocytes and the number of mature oocytes in gonadotropin-releasing hormone agonist (GnRHa) + human menopausal gonadotropin (hMG) + hCG and hMG + hCG cycles were higher than those in the natural cycle (P < 0.0001). The rate of mature oocytes in hMG + hCG cycle was the highest among the three protocols (P < 0.04). In the mural granulosa cells, the incidence of apoptotic cells in the GnRHa + hMG + hCG cycle was significantly higher than those of the natural (P < 0.002) and hMG + hCG cycles (P = 0.0002). The incidence of apoptotic cumulus granulosa cells in the GnRHa + hMG + hCG cycle was significantly higher than those of natural and hMG + hCG cycles (P < 0.002). Moreover, the incidence of apoptotic cumulus granulosa cells in the hMG + hCG cycle was significantly lower than that in the natural cycle (P < 0.01).

Conclusions: These results indicated that hMG + hCG is the most appropriate controlled ovarian hyperstimulation protocol among the three examined with regard to oocyte quality.

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REFERENCES

  1. Garcia JE, Jones GS, Acosta AA, Wrigt G: Human menopausal gonadotropin/human chorionic gonadotropin follicular maturation for oocyte aspiration: Phase 1. Fertil Steril 1983;39:174-179

    Google Scholar 

  2. Laufer N, DeCherney AH, Haseltine FP, Polan ML, Mezer HC, Dlugi AM, Sweeney D, Nero F, Naftolin F: The use of high-dose human menopausal gonadotropin in an in vitro fertilization program. Fertil Steril 1984;40:734-741

    Google Scholar 

  3. DeCherney AH, Tarlatzis BC, Laufer N: Follicular development: Lessons learned from human in vitro fertilization. Am J Obstet Gynecol 1985;153:911-923

    Google Scholar 

  4. Porter RN, Smith W, Craft IL, Abdulwahid NA, Jacobs HS: Induction of ovulation for in vitro fertilization using buserelin and gonadotropins. Lancet 1984;2:1284-1285

    Google Scholar 

  5. Fleming R, Haxton MJ, Yates RWS: A simple procedure for the induction of multiple follicular growth with blockade of LH surge in normal women. In Program and Abstracts of the Annual Meeting of the Endocrine Society. Baltimore, Maryland, 1985, pp

  6. Nader S, Berkowitz AS, Maklad N, Wolf DP, Held B: Characteristics of patients with and without gonadotropin surges during follicular recruitment in an in vitro fertilization/embryo transfer program. Fertil Steril 1986;45:75-78

    Google Scholar 

  7. Rutherford AJ, Subak-Sharpe RJ, Dawson KJ, et al.: Improvement of in vitro fertilization after treatment with buserelin, an agonist of luteinising hormone releasing hormone. Br Med J 1988;296:1765-1768

    Google Scholar 

  8. Billig H, Furuta I, Hsueh AJW: Gonadotropin-releasing hormone directly induces apoptotic cell death in the rat ovary: Biochemical and in situ detection of deoxyribonucleic acid fragmentation in granulosa cells. Endocrinology 1994; 134:245-252

    Google Scholar 

  9. Testart J, Belaisch-Allart J, Frydman R: Relationship between embryo transfer results and ovarian response and in-vitro fertilization rate: Analysis of 186 human pregnancies. Fertil Steril 1986;45:237-243

    Google Scholar 

  10. Testart J, Belaisch-Allart JC, Forman R, Gazengel A, Strubb N, Hazout A, Strubb N, Frydman R: Influence of different stimulation treatment on oocyte characteristics and in-vitro fertilizing ability. Human Reprod 1989;4:22-29

    Google Scholar 

  11. Tilly JL, Kowalski KI, Johnson AL, Hsueh AJW: Involvement of apoptosis in ovarian follicular atresia and postovulatory regression. Endocrinology 1991;129:2799-2801

    Google Scholar 

  12. Piquette GN, Tilly JL, Prichard L, Simon C, Polan ML: Detection of apoptosis in human and rat ovarian follicles. J Soc Gynecol Invest 1994;1:297-301

    Google Scholar 

  13. Leoncini L, Del Vecchio MT, Megha T, Barbini P, Galieni P, Pileri S, Sabattini E, Gherlinozi F, Tosi P, Kraft R, Cotier H: Correlation between apoptotic and proliferative indices in malignant non-Hodgkin's lymphoma. Am J Pathol 1993;142:755-763

    Google Scholar 

  14. Wheeler TM, Rogers E, Aihara M, Scardino PT, Thompson TC: Apoptotic index as a biomaker in prostatic intraepithelial neoplasma (PIN) and prostatic cancer. J Cell Biol 1994;19:202-207

    Google Scholar 

  15. Nakahara K, Saito H, Saito T, Ito M, Ohta N, Sakai N, Tezuka N, Hiroi M: Incidence of apoptotic bodies in membrana granulosa of the patients participating in an in vitro fertilization programs. Fertil Steril 1997;67:302-308

    Google Scholar 

  16. Nakahara K, Saito H, Saito T, Ito M, Ohta N, Takahashi T, Hiroi M: Incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs. Fertil Steril 1997;68:312-317

    Google Scholar 

  17. Nakahara K, Saito H, Saito T, Ito M, Ohta N, Takahashi T, Hiroi M: Ovarian fecundity in patients with endometriosis can be estimated by the incidence of apoptotic bodies. Fertil Steril 1998;69:931-935

    Google Scholar 

  18. Saito H, Hiroi M: Correlation between the follicular gonadotropin inhibitor and the maturity of ovum-corona-cumulus complex. Fertil Steril 1986;46:66-72

    Google Scholar 

  19. Neveu S, Hedon B, Bringer J, Chinchole JM, Arnal F, Humeau C, Cristol P, Viala JL: Ovarian stimulation by a combination of gonadotropin-releasing hormone agonist and gonadotropins for in vitro fertilization. Fertil Steril 1987;47:639-643

    Google Scholar 

  20. Ashkenazi J, Dicker D, Feldberg D, Goldman GA, Yeshaya A, Goldman JA: The value of GnRH analogue therapy in Journal of Assisted Reproduction and Genetics, Vol. 17, No. 10, 2000 IVF in women with unexplained infertility. Hum Reprod 1989;4:667-669

    Google Scholar 

  21. Zhao S, Saito H, Wang X, Saito T, Kaneko T, Hiroi M: Effects of gonadotropin-releasing hormone agonist on the incidence of apoptosis in porcine and human granulosa cells. Gynecol Obstet Invest 2000;49:52-56

    Google Scholar 

  22. Saito S, Saito H, Yanasaki E, Hosoi E, Komatsu M, Iwahana H, Maeda T: Radioimmunoassay of an analog of luteinizing hormone-releasing hormone, [D-Ser (tBu)]6 des-Gly-NH2 10 ethylamide (Buserelin). Immunol Methods 1985;79:173-183

    Google Scholar 

  23. Friedman CI, Schmidt CE, Chang FE, Kim MH: Severe ovarian hyperstimulation following follicular aspiration. Am J Obstet Gynecol 1984;150:436-437

    Google Scholar 

  24. Serhal PF, Katz M, Little V, Woronowski H: Unexplained infertility-The value of Pergonal superovulation combined with intrauterine insemination. Fertil Steril 1988;49:602-606

    Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata city, Yamagata, 990-9585, Japan

    Tomoko Kaneko, Hidekazu Saito, Toshifumi Takahashi, Nobuhiko Ohta, Takakazu Saito & Masahiko Hiroi

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  1. Tomoko Kaneko
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  2. Hidekazu Saito
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  3. Toshifumi Takahashi
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  4. Nobuhiko Ohta
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  5. Takakazu Saito
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  6. Masahiko Hiroi
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Kaneko, T., Saito, H., Takahashi, T. et al. Effects of Controlled Ovarian Hyperstimulation on Oocyte Quality in Terms of the Incidence of Apoptotic Granulosa Cells. J Assist Reprod Genet 17, 580–585 (2000). https://doi.org/10.1023/A:1026439409584

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