Abstract
Papio hamadryas baboons in the Sukhumi colony develop enzootic outbreaks of malignant lymphomas with an incidence of about 1.5% per year among adults of the high-risk stock. We investigated the surface phenotypes of cells from normal and lymphomatous animals using antibodies against human lymphocyte antigens. We found that more than 80% of the lymphomas that developed during the last 3 years were characterized histologically to be of the peripheral T cell type. Generally, the lymphomatous cells also expressed high levels of MHC class II DR protein, CD18 (LFA-1 beta chain), and CD45RO. Surprisingly, these cells also expressed on their surface two proteins previously characterized as being relatively B cell-restricted: CD40 and Bgp95. These proteins were never found on the peripheral blood T cells from normal animals. The expression of these two gene products was confirmed by RNA blotting and immunoprecipitation. In most cases, the two B cell-associated proteins were expressed on the predominant T cell subsets; we found both B cell proteins on CD4+, CD8+ as well as on the CD4/8 double-positive cells when these subsets were expressed at high levels. About 90% of these animals are seropositive forHerpesvirus papio and human T cell leukemia virus-1 (HTLV-1) before developing outright lymphomas. In all of the lymphoma samples, HTLV-1 tax DNA sequences were detected by PCR amplification. Whether or not HTLV-1 or theHerpesvirus papio gene products influence the surface expression of CD40 and Bgp95 remains to be determined.
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References
Lapin B: Baboon lymphomas viruses.In Virus Diseases in Laboratory and Captive Animals, G Darai (ed). Boston, MA, Martinus Nijhoff, 1988, pp 135–151
Voevodin AF, Lapin BA, Yakovleva LA, Ponomaryeva TI, Oganyan TE, Razmadze EN: Antibodies reacting with human T-lymphotropic retrovirus (HTLV-1) or related antigens in lymphomatous and healthy hamadryas baboons. Int J Cancer 36:579–584, 1985
Yakovleva LA, Bukaeva IA, Krylova RT, Markova TP: Pathomorphological ultrastructural cytochemical and immunological characteristics of baboon hemoblastosis in Sukhumi monkey stock.In Advances in Comparative Leukemia Research, B Lapin (ed). Moscow, 1980, pp 594–599
Yakovleva LA, Bukaeva IA, Indzhiia LV: Classification of nonHodgkin's malignant lymphomas NHLs in baboons at the Sukhumi monkey colony. Vestnik AMS USSR 10:28–32, 1987
Indzhiia LV, Yakovleva LA, Chikobava MB, Valentine MA, Lapin BA, Clark EA: Non-Hodgkin's malignant lymphomas of primates. Immunodiagnostic approaches to T-cell NHL. Vestnik AMN USSR 9:21–26, 1990
Clark EA, Draves KE: Activation of macaque T cells and B cells with agonistic monoclonal antibodies. Eur J Immunol 17:1799–1805, 1987
Indzhiia LA, Chikobava MG, Lazareva IY, Tsaturyan TN: Cross reactivity of lymphocytes freom P. hamadryas and three species of macacas with some monoclonal antibodies to differentiated antigens of human T lymphocytes and to la-like antigens.In Medical Primatology. The Main Perspective Uses of Primates in Medicine and Biology, BA Lapin (ed). Sukhumi, Russ, 1987, pp 167–170
Jonker M, Singerland W: Reactivity of mAb specific for human CD markers with rhesus monkey leucocytes.In Leukocyte Typing IV. W Knapp (ed). Oxford, Oxford University Press, 1990, pp 1058–1063
Lane PJL, Valentine MA, Barrett T, McConnell F, Meier KE, Clark EA: Surface molecules and signal transduction events in human B cells.In Ligands, Receptors and Signal Transduction in Regulation of Lymphocyte function, J Cambier (ed). American Society for Microbiology, Washington, DC, 1990, pp 117–143
Paulie S, Rosen A, Ehlin-Henricksson B, Braesch-Andersen S, Jakobsen E, Koho H, Perlmann P: A p50 surface antigen restricted to human urinary bladder carcinomas and B lymphocytes. Cancer Immunol Immunother 20:23–28, 1985
Clark EA: CD40: A cytokine receptor in research of a ligand. Tissue Antigens 35:33–36, 1990
Valentine MA, Clark EA, Shu GL, Norris NA, Ledbetter JA: Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation. J Immunol 140:4071–4078, 1988
Dunlap NE, Berry AK, Gore I, Decker WD, Snyder TL, Shaw GM, Hanh BH, Tildern AB: Expression of activation antigens on HTLV-1 and THLV-II cell lines.In Leucocyte Typing IV, W. Knappet al. (eds). Oxford, Oxford University Press, 1989, pp 487–488
Naruyama M, Shibuy H, Harada H, Hatakeyama M, Seiki M, Fujita T, Inoue J, Yoshida M, Taniguchi T: Evidence for aberrant activation of the interleukin-2 autocrine loop by HTLV-1 encoded p40 and T3/Ti complex triggering. Cell 48:343–350, 1987
Jeffery SW, Sielicki M, Haxo FT: Chloroplast pigment patterns in dinoflagellates. J Phycol 11:374, 1975
Prezelin BB, Haxo FT: Purification and characterization of peridinin-chlorophyll A proteins from the marine dinoflagellatesGlendium sp. andGonyaulax polydera. Plant (Berl.) 128:133, 1976
Afar B, Merrill J, Clark EA: Use of chlorophyll A protein (perCP)-monoclonal antibody conjugates for simplified three colour flow cytometric analyses. J Clin Immunol 11:254–261, 1991
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685, 1970
Chomczynski P, Sacch N: Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159, 1987
Stamenkovic I, Clark EA, Seed B: A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas. EMBO J 8:1403–1410, 1989
Lee H, Swanson P, Shorty VS, Zack JA, Rosenblatt JD, Chen ISY: High rate of HTLV-II infection in seropositive IV drug abusers in New Orleans. Science 244:471–475, 1989
Seiki M, Hattori S, Yoshida M: Human adult T-cell leukemia virus: Molecular cloning of the provirus DNA and the unique terminal structure. Proc Natl Acad Sci USA 79:6899–6902, 1982
Ramsay AD, Smith WJ, Earl HM, Souhami RL, Issacson PG: T-cell lymphomas in adults: A clinicopathological study of eighteen cases. J Pathol 152:63–78, 1987
Picker LJ, Weiss LM, Medieros LJ, Wood GS, Warnke RA: Immunophenotypic criteria for the diagnosis of Non-Hodgkins lymphoma. Am J Pathol 128:181–201, 1987
Doi S, Nasu K, Arita Y, Tanabe S, Matsuyama F, Kamesaki H, Fukyhara S, Nishikori M, Miwa H, Kita K, Hatanaka M, Uchino H: Immunohistochemical analysis of peripheral T-cell lymphoma in Japanese patients. Am J Clin Pathol 91:152–158, 1989
Grogan TM, Fiedler K, Rangel C, Jolley CJ, Wirt DP, Hicks MJ, Miller TP, Brooks R, Greenberg B, Jones S: Peripheral T-cell lymphoma: Aggressive disease with heterogenous immunotypes. Am J Clin Pathol 83:279–288, 1985
Krajewski AS, Myskow MW, Cachia PG, Salter DM, Sheehan T, Dewar AE: T-cell lymphoma: Morphology, Immunophenotype and clinical features. Histopathology 13:19–41, 1988
Coiffier F, Berger F, Byron P, Magaud J: T-cell lymphomas: Immunologic, histologic, clinical and therapeutic analysis of 63 cases. J Clin Oncol 6:1584–1589, 1990
Springer TA: Adhesion receptor of the immune system. Nature 346:425–434, 1990
Suchi T, Lennert K, Tu L-Y, Kikuchi M, Sate E, Stansfeld AG, Feller AC: Histopathology and immunohistochemistry of peripheral T cell lymphomas: A proposal for their classification. J Clin Pathol 40:995–1015, 1987
Watanabe S, Mukai K, Shimoyama M: Peripheral T-cell lymphoma. Cancer Metastat Rev 7:243–261, 1988
Stansfeld AG, Diebold F, Kapanci Y, Kelenyi G, Lennert K, Miduszewska O, Noel H, Rilke F, Sundstrom C, van Unnik JAM, Wright DH: Updated Kiel classification for lymphomas. Lancet 6:292–293, 1988
Alexander C, Verrier B: Four regulatory elements in the human c-fos promoter mediate transactivation by HTLV-1 Tax protein. Oncogene 6:543–551, 1991
Farcet JP, Lebargy F, Lavignac C, Gaular P, Dautry A, Gazzolo L, Romeo P, Vainchender W: Constitutive IL-2 expression in HTLV-1-infected leukaemic T cell lines. Clin Exp Immunol 84:415–421, 1991
Clark EA, Shu G: Association between IL-6 and CD40 signaling. J Immunol 145:1400–1406, 1990
Nishimoto N, Yoshizaki K, Eiraku M, Machigashira K, Tagoh H, Ogata A, Kuritani T, Same M, Kishimoto T: Elevated levels of interleukin-6 in serum and cerebrospinal fluid of HTLV-1-associated myelopathy/tropical spastic paraparesis. J Neurol Sci 97:183–193, 1990
Villiger PM, Cronin MT, Amenomori T, Wachsman W, Lotz M: IL-6 production by human T lymphocytes. Expression in HTLV-2-infected but not in normal T cells. J Immunol 146:550–559, 1991
Dhawan S, Streicher HZ, Wahl LM, Miller N, Louie AT, Goldfard IS, Jackson WL, Casali P, Notkins AL: Model for studying virus attachment. II. Binding of biotinylated human T cell leukemia virus type I to human blood mononuclear cells potential targets for human T cell leukemia virus type I infection. J Immunol 147:102–108, 1991
Ng VL, Ranier C, Oliver MR, Marsh J, Wood P, Reys GR, McGrath MS: IgM secreted by human T lymphoma virus-1 infected and transformed human B cell clones recognized by a 66-kilodalton host-encoded protein. J Immunol 143:2501–2507, 1989
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Indzhiia, L.V., Yakovleva, L.A., Overbaugh, J. et al. Baboon T cell lymphomas expressing the B cell-associated surface proteins CD40 and Bgp95. J Clin Immunol 12, 225–236 (1992). https://doi.org/10.1007/BF00918093
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DOI: https://doi.org/10.1007/BF00918093