Skip to main content
SpringerLink
Log in

Characterization of Antifungal Metabolites Produced by Penicillium Species Isolated from Seeds of Picea glehnii

  • Published:
Journal of Chemical Ecology Aims and scope Submit manuscript

Abstract

We screened the microorganisms that are present on the surface of Picea glehnii seeds and produced antifungal compounds against Pythium vexans, a fungus that causes damping-off. Four isolates of Penicillium species that produced patulin, citrinin, palitantin, and arthrographol, respectively, were identified from 149 different microorganisms screened. This study is the first step in an examination of the ecological interaction between host conifers and fungi located on the surface of their seeds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  • Anslow, W. K., Raistrick, H., and Smith, G. 1943. Anti-fungal substances from moulds. Part I. Patulin (anhydro-3-hydroxymethylene-tetrahydro-1:4-pyrone-2-carboxylic acid), a metabolic product of Penicillium patulum Bainier and Penicillium expansum (Link) Thom. J. Soc. Chem. Ind. 62:236-238.

    Google Scholar 

  • Ayer, W. A., and Nozawa, K. 1990. Taxonomy and chemistry of a new fungus from bark beetle infested Pinus contorta var. latifolia. Part 2. Arthrographol, the metabolite inhibitory to Ophiostoma clavigerum. Can. J. Microbiol. 36:83-85.

    Google Scholar 

  • Betina, V. 1984. Mycotoxins—Production, Isolation, Separation and Purification. Elsevier, Amsterdam, p. 217-236, 291–314.

    Google Scholar 

  • Betina, V. 1989. Mycotoxins: Chemical, Biological and Environmental Aspects. Elsevier, Amsterdam, pp. 242-269.

    Google Scholar 

  • Birkinshaw, J. H., and Raistrick, H. 1936. CXVIII. Studies in the biochemistry of micro-organisms. XLIX. Palitantin, C14H22O4, a hitherto undescribed metabolic product of Penicillium palitans Westling. Biochem. J. 30:801-808.

    Google Scholar 

  • Bracken, A., Pocker, A., and Raistrick, H. 1954. Studies in the biochemistry of micro-organisms. 93. Cyclopenin, a nitrogen-containing metabolic product of Penicillium cyclopium Westling. Biochem. J. 57:587-595.

    Google Scholar 

  • Chakravarty, P., and Unestam, T. 1987. Differential influence of ectomycorrhizae on plant growth and disease resistance in Pinus sylvestris seedlings. J. Phytopathol. 120:104-120.

    Google Scholar 

  • Colombo, L., Gennari, C., Ricca, G. S., and Scolastico, C. 1980. Biosynthetic origin and revised structure of ascochitine, a phytotoxic fungal metabolite. Incorporation of [1-13C]-and [1,2-13C2]acetates and [Me-13C]methionine. J. Chem. Soc. Perkin Trans. I 1980:675-676.

    Google Scholar 

  • Curtis, P. J., Hemming, H. G., and Smith, W. K. 1951. Frequentin: An antibiotic produced by some strains of Penicillium frequentans Westling. Nature 167:557-558.

    Google Scholar 

  • Damodaran, C., Kathirvel-Pandian, S., Seeni, S., Selvam, R., Ganesan, M. G., and Shanmugasundaram, S. 1975. Citrinin, a phytotoxin? Experientia 31:1415-1417.

    Google Scholar 

  • Duchesne, L. C., and Peterson, R. L. 1988. Interaction between the ectomycorrhizal fungus Paxillus involutus and Pinus resinosa induces resistance to Fusarium oxysporum. Can. J. Bot. 66:558-562.

    Google Scholar 

  • Duchesne, L. C., Peterson, R. L., and Ellis, B. E. 1988. Pine root exudate stimulates the synthesis of antifungal compounds by the ectomycorrhizal fungus Paxillus involutus. New Phytol. 108:471-476.

    Google Scholar 

  • Duchesne, L. C., Ellis, B. E., and Peterson, R. L. 1989. Disease suppression by the ectomycorrhizal fungus Paxillus involutus: Contribution of oxalic acid. Can. J. Bot. 67:2726-2730.

    Google Scholar 

  • Hanessian, S., Sakito, Y., Dhanoa, D., and Baptistella, L. 1989. Synthesis of (+)-palitantin. Tetrahedron 45:6623-6630.

    Google Scholar 

  • Homans, A. L., and Fuchs, A. 1970. Direct bioautography on thin-layer chromatograms as a method for detecting fungitoxic substances. J. Chromatogr. 51:327-329.

    Google Scholar 

  • Ingham, J. L. 1973. Disease resistance in higher plants. The concept of pre-infectional and post-infectional resistance. Phytopath. Z. 78:314-335.

    Google Scholar 

  • Ito, K. 1955. Jyubyo-kougi. Chikyu-syuppansya, Tokyo. pp. 19-30 (in Japanese).

    Google Scholar 

  • Kasuya, M. K. M. 1995. Ecological and physiological studies on ectomycorrhizae of Picea glehnii (Fr. Schm.) Masters. Doctoral thesis. Graduate School of Agriculture, Hokkaido University, Sapporo.

    Google Scholar 

  • Katzman, P. A., Hays, E. E., Cain, C. K., Van Wyk, J. J., Reithel, F. J., Thayer, S. A., and Doisy, E. A. 1944. Clavacin, an antibiotic substance from Aspergillus clavatus. J. Biol. Chem. 154:475-486.

    Google Scholar 

  • Marx, D. H. 1972. Ectomycorrhizae as biological deterrents to pathogenic root infections. Annu. Rev. Phytopathol. 10:429-454.

    Google Scholar 

  • Mierisova, S., Proksa, B., and Liptaj, T. 1996. 1H and 13C NMR data for palitantin and its derivatives. Magn. Reson. Chem. 34:414-415.

    Google Scholar 

  • Nickell, L. G., and Finlay, A. C. 1954. Antibiotics and their effects on plant growth. J. Agric. Food Chem. 2:178-182.

    Google Scholar 

  • Okeke, B., Seigle-Murandi, F., Steiman, R., Benoit-Guyod, J. L., and Kaouadji, M. 1993. Identification of mycotoxin-producing fungal strains: A step in the isolation of compounds active against rice fungal diseases. J. Agric. Food Chem. 41:1731-1735.

    Google Scholar 

  • Paterson, R. R. M., and Kemmelmeier, C. 1990. Neutral, alkaline and difference ultraviolet spectra of secondary metabolites from Penicillium and other fungi, and comparisons to published maxima from gradient high performance liquid chromatography with diode-array detection. J. Chromatogr. 511:195-221.

    Google Scholar 

  • Pfefferle, W., Anke, H., Bross, M., and Steffan, B. 1990. Asperfuran, a novel antifungal metabolite from Aspergillus oryzae. J. Antibiot. 43:648-654.

    Google Scholar 

  • Ramirez, C. 1982. Manual and atlas of the Penicillia. Elsevier Biomedical, Amsterdam, pp. 235-286.

    Google Scholar 

  • Robinson, P. M., and Park, D. 1966. Citrinin—a fungistatic antibiotic and narrowing factor. Nature 211:883-884.

    Google Scholar 

  • Shimazu, A. 1986. Antibiotics, p. 754, in Yamazato, K., Udagawa, S., Kodama, T. and Shinchi, T. (eds.). Biseibutu-no douteihou. R&D Planning, Tokyo (in Japanese).

    Google Scholar 

  • Stack, R. W., and Sinclair, W. A. 1975. Protection of Douglas-fir seedlings against Fusarium root rot by a mycorrhizal fungus in the absence of mycorrhiza formation. Phytopathology 65:468-472.

    Google Scholar 

  • Takahashi, I. 1980. The research for fungi which appeared during natural regeneration of Picea jezoensis and Abies sachalinensis. Trans. Meet. Hokkaido Br. Jpn. For. Soc. 29:125-127 (in Japanese).

    Google Scholar 

  • Tatewaki, M. 1958. Forest ecology of the islands of the North Pacific Ocean. J. Fac. Agric. Hokkaido Univ. 50:371-486.

    Google Scholar 

  • Wang, F. H. 1948. The effects of clavacin upon root growth. Bot. Bull. Acad. Sinica 2:265-269.

    Google Scholar 

  • Watanabe, T. 1993. Dojyou-shijyoukin. Softscience-sya. Tokyo, pp. 1-5 (in Japanese).

    Google Scholar 

  • White, A. G., and Truelove, B. 1972. The effects of aflatoxin B1, citrinin, and orchratoxin A on amino acid uptake and incorporation by cucumber. Can. J. Bot. 50:2659-2664.

    Google Scholar 

  • Zak, B. 1964. Role of mycorrhizae in root disease. Annu. Rev. Phytopathol. 2:377-392.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo, 060-8589, Japan

    K. Yamaji, Y. Fukushi, Y. Hashidoko & S. Tahara

  2. CREST, Japan Science and Technology Corporation, Honmachi 4-1-8, Kawaguchi, 332-0012, Japan

    K. Yamaji, Y. Fukushi, Y. Hashidoko & S. Tahara

  3. Department of Food and Nutrition, Hokkaido Bunkyou Junior College, Kogane-cho 196, Eniwa, 061-1408, Japan

    T. Yoshida

Authors
  1. K. Yamaji
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Fukushi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Hashidoko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Tahara
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamaji, K., Fukushi, Y., Hashidoko, Y. et al. Characterization of Antifungal Metabolites Produced by Penicillium Species Isolated from Seeds of Picea glehnii . J Chem Ecol 25, 1643–1653 (1999). https://doi.org/10.1023/A:1020849202413

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1020849202413

Search

Navigation