Abstract
The existence and expression of gene encoding the Ca2+-binding protein regucalcin in various species and tissues were investigated with Southern and Northern hybridization analyses using regucalcin cDNA (0.9 kb of open reading frame). Genomic Southern hybridization analysis demonstrated that regucalcin gene was widely conserved among higher animals including human, monkey, rat, mouse, dog, bovine, rabbit and chicken. The gene was not found in yeast. The Northern blot analysis of poly (A)+RNAs extracted from the liver of various species showed that regucalcin mRNA was predominantly expressed in rat and mouse, although the expression was also seen in human, bovine and chicken. Furthermore, the enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG indicated that hepatic regucalcin concentration was most pronounced in rat as compared with that of guinea pig, mouse and chicken. These observations show that the gene expression of regucalcin and its protein synthesis is unique in the liver of rats, suggesting the existence of a specific mechanism in demonstrating regucalcin synthesis from gene.
Similar content being viewed by others
References
Wasserman RH: Calcium-binding proteins: Past, present and future. In: R.H. Wasserman (ed.). Novel Calcium-Binding Proteins. Springer-Verlag, Berlin, pp 7–12, 1991
Yamaguchi M, Yamamoto T: Purification of calcium binding substance from soluble fraction of normal rat liver. Chem Pharm Bull 26: 1915–1918, 1978
Yamaguchi M, Sugii K: Properties of calcium-binding protein isolated from the soluble fraction of normal rat liver. Chem Pharm Bull 29: 567–570, 1981
Yamaguchi M, Yoshida H: Regulatory effect of calcium-binding protein isolated from rat liver cytosol on activation of fructose, 1, 6-diphosphatase by Ca2+-calmodulin. Chem Pharm Bull 33:4489–4493, 1985
Yamaguchi M, Mori S: Inhibitory effect of calcium-binding protein regucalcin on protein kinase C activity in rat liver cytosol. Biochem Med Metab Biol 43:140–146, 1990
Mori S, Yamaguchi M: Hepatic calcium-binding protein regucalcin decreases calcium calmodilin-dependent protein kinase activity in rat liver cytosol. Chem Pharm Bull 38:2216–2218, 1990
Yamaguchi M, Tai H: Inhibitory effect of calcium-binding protein regucalcin on Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase activity in rat liver cytosol. Moll Cell Biochem 106:25–30, 1991
Yamaguchi M, Sakurai T: Inhibitory effect of calcium-binding protein regucalcin on Ca2+-activated DNA fragmentation in rat liver nuclei. FEBS Lett 279:281–284, 1991
Shimokawa N, Yamaguchi M: Molecular cloning and sequencing of the cDNA coding for a calcium-binding protein regucalcin from rat liver. FEBS Lett 327:251–255, 1993
Shimokawa N, Yamaguchi M: Expression of hepatic calcium-binding protein regucalcin mRNA is mediated through Ca2+/calmodulin in rat liver. FEBS Lett 316:79–84, 1993
Feinberg A, Vogelstein B: A technique for labelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983
Sambrook J, Maniatis T, Fritsch EF: Northern hybridization. In: Molecular Cloning (2nd Edn), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 7.39–7.52, 1989
Yamaguchi M, Isogai M: Tissue concentration of calcium-binding protein regucalcin in rats by enzyme-linked immunoadsorbent assay. Moll Cell Biochem 122:65–68, 1993
Fujita T, Shirasawa T, Uchida K, Maruyama N: Isolation of cDNA clone encoding rat senescence marker protein-30 (SMP30) and its tissue distribution. Biochim Biophys Acta 1132:297–305, 1992
Perler F, Efstratiadis A, Lomedico P, Gilbert W, Kolonder R, Dodgson J: The evolution of genes: the chicken preproinsulin gene. Cell 20: 555–566, 1980
Shimokawa N, Yamaguchi M: Calcium administration stimulates the expression of calcium-binding protein regucalcin mRNA in rat liver. FEBS Lett 305:151–154, 1992
Dinauer M, Pierce E, Bruns G, Curnutte J, Orkin S: Human neutrophi cytochrome b light chain (p22-phox). J Clin Invest 86:1729–1737, 1990
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shimokawa, N., Isogai, M. & Yamaguchi, M. Specific species and tissue differences for the gene expression of calcium-binding protein regucalcin. Mol Cell Biochem 143, 67–71 (1995). https://doi.org/10.1007/BF00925928
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00925928