Abstract
Possible involvement of cardiac renin-angiotensin system (RAS) in pressure overload induced left ventricular hypertrophy (LVH) was investigated. Rats were subjected to abdominal aortic constriction (AAC) and examined the effects of 4 weeks treatments with an angiotensin converting enzyme (ACE) inhibitor, captopril and a vasodilator, hydralazine on haemodynamics and ventricular RNA, DNA, protein and myosin isoform pattern in sham and hypertrophied rats. AAC increased the mean arterial pressure (MAP) and systolic blood pressure (SBP), and resulted in increased left ventricle/body weight ratio, LV thickness, RNA and protein content, however total DNA was not changed. The expression of fetal isogene, β-myosin heavy chain (β-MHC), was markedly enhanced where as u.-MHC was reduced. High-dose captopril (100 mg/kg p.o.,) significantly prevented the increase in haemodynamics, development of LVH, LV remodeling, increase in total protein, RNA and antithetical expression of myosin isoforms. Hydralazine (15 mg/kg p.o.,), did not modulate hypertrophic changes and low-dose captopril (1.5 mg/kg p.o.,) which has not produced any marked fall in MAP and SBP also modulated favourably the development of LVH and its biochemical markers. Thus, the prevention of the development of LVH and induction of β-MHC by non-hypotensive doses of captopril may be related to the blockade of intracardiac production of angiotensin II rather than circulating system. These results suggest that cardiac RAS may play an important role in pressure overload induced LVH.
References
Lindpaintner K, Ganten D: The Cardiac renin-angiotensin system: An appraisal of present experimental and clinical evidence. Circ Res 68: 905–21, 1991
Pfeffer JM, Pfeffer MA, Mirsky I, Braunwald E: Regression of LVH and prevention of LV dysfunction by captopril in the spontaneously hypertensive rats. Proc Natl Acad Sci USA 79: 3310–14, 1982
Re RN: The cellular biology of angiotensin: Paracrine, autocrine and intracrine actions in cardiovascular tissues. J Mol Cell Cardiol 21 (Suppl. V): 63–69, 1989
Baker KM, Booz GW, Dostal DE: Cardic actions of angiotensin II: Role of an intracardiac renin-angiotensin system. Anon Rev Physiol 54: 227–41, 1992
Sen S, Tarazi RC, Khairallah PA, Bumpus FM: Cardiac hypertrophy in spontaneously hypertensive rats. Circ Res 35: 775–81, 1974
Linz W, Schoelkens BA, Ganten D: Converting enzyme inhibition specifically prevents the development and induces the regression of cardiac hypertrophy in rats. Clin Exp Hypertens 11: 1325–35, 1989
Khairallah PA, Kanabus J: Angiotensin and myocardial protein synthesis. In: R.D. Tarazi, J.B. Dunbar (eds). Perspectives in Cardiovascular Research. Raven Press, New York, 1983: 8, pp-337–17
Baker KM: Cardiac actions of angiotensin. J Vasc Med Biol 3: 30–37, 1991
Beinlich CJ, White GJ, Baker KM, Morgan HE: Angiotensin-II and left ventricular growth in newborn pig heart. J Mol Cell Cardiol 23: 1031–38, 1991
Baker KM, Aceto JF: Angiotensin II stimulation of protein synthesis and cell growth in chick heart cells. Am J Physiol 259: H610–18, 1990
Sadoshima J, Izumo S: Molecular characterization of angiotensin II-induced hypertrophy of cardiac myocytes and hyperplasia of cardiac fibroblasts: critical role of the AT, receptor subtype. Circ Res 73: 413–23, 1993
Aceto JF, Baker KM: (Sar1) angiotensin II receptor-mediated stimulation of protein synthesis in chick heart cells. Am J Physiol 258: H806–13, 1990
Sadoshima J, Izumo S: Signal transduction pathways of angiotensin II induced c-fos gene expression in cardiac myocytesin vitro: Roles of phospholipid derived second messengers. Circ Res 73: 424–38, 1993
Schunkert H, Dzau VJ, Tang SS, Hirsch AT, Apsutein CS, Lorell BH: Increased rat cardiac angiotensin converting enzyme activity and mRNA expression in pressure overload left ventricular hypertrophy: effects on coronary resistance, contractility, and relaxation. J Clin Invest 86: 1916–20, 1991
Baker KM, Chernin MI, Wixson SK, Aceto JF: Renin-angiotensin system involvement in pressure overload cardiac hypertrophy. Am J Physiol 259: H324–32, 1990
Kromer EP, Riegger GAP: Effects of long-term angiotensin converting enzyme inhibition on myocardial hypertrophy in experimental aortic stenosis in the rat. Am J Cardiol 62: 161–63, 1988
Kromer EP, Elsner X, Riegger GAJ: Role of neurohumoral systems for pressure induced left ventricular hypertrophy in experimental supravalvular aortic stenosis in rats. J Hypertens 4: 521–24, 1991
Zierhut W, Zimmer HG, Gerdes AM: Effect of angiotensin converting inhibition on pressure-induced left ventricular hypertrophy in rats. Circ Res 69: 609–17, 1991
Ruzika M, Yuan B, Harmsen E, Leenan FHH: The renin angiotensin system and volume overload-induced cardiac hypertrophy in rats. Circulation 87: 921–930, 1993
Urata H, Healy, B, Stewart RW, Bumpus FM, Husain A: Angiotensin II forming pathways in normal and failing human hearts. Circ Res 66: 883–90, 1990
Kohya T, Kimura SK, Myerburg RJ, Bassett AL: Susceptibility of hypertrophied rat hearts to ventricular fibrillation during acute ischemia. J Mol Cell Cardiol 20: 159–68, 1988
Leenan FHH, Prowse S: Time-course of changed hypertrophy and pressor mechanisms in two-kidney, one clip hypertensive rats during treatment with minoxidil, enalapril or after uninephrectomy. J Hypertens 5: 73–83, 1987
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin-Phenol reagent. J Biol Chem 193: 265–75, 1951
Burton R: A study of the conditions and mechanism of the diphenylamine reaction for the colorometric estimation of deoxyribonucleic acid. Biochem J 62: 315–22, 1956
Richards GM: Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal Biochem 57: 369–76, 1974
Chomozynski P, Sacchi N: A single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–59, 1987
Esser KA, Boluyt M0, White TP: Separation of cardiac myosin heavy chains by gradient SDS-PAGE. Am J Physiol 255: H659–63, 1988
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature Lond 227: 680–85, 1970
Chopra K, Singh M, Kaul N, Ganguly NK: Oxygen free radicals and protective effect of captopril on myocardial infarct size. Arch Int Pharmacodyn 322:55–65, 1993
Clozel J-P, Saunier C, Harteman D, Fischli W: Effects of cilazapril a novel angiotensin converting enzyme inhibitor, on the structure of pulmonary arteries of rats exposed to chronic hypoxia. J Cardiovasc Pharmacol 17: 36–40, 1991
Kellet R, Gales AC, Fraser-Rae L, Jackson C, Richard D: Factors other than renin-angiotensin-aldosterone system (RAAS) are important in controlling left ventricular hypertrophy (Abstr.). J Mol Cell Cardiol 25 (Suppl. III): S-52, 1993
Msutomo, Hata T, Yanaga T: Effects of ACE inhibitor on regression of cardiac hypertrophy (Abstr.). J Mol Cell Cardiol 25 (Suppl. II): S34, 1993
Brilla CG, Pick R, Tan LB, Janicki JS, Weber KT: Remodelling of rat right and left ventricles in experimental hypertension. Circ Res 67: 1355–64, 1990
Brilla CG, Weber KT: Myokardifibrose bei art. Hypertonie: Regression via Geweb-RAS-Hemmung (Abstr.). Z Kardiol 80 (Suppl. III): 164 1991
Nadal-Ginard B, Mahdavi V: General principles of Cardiovascular Cellular and Molecular Biology, In: E Braunwald (ed.). Heart Disease. WB Saunders Company, Philadelphia, 1992, pp 1602–21
Oka N, Nakata M, Tanaka M, Sumida E, Chiba M, Koga Y, Toshima H: Role of adrenergic and angiotensin systems in the development of pressure-overload cardiac hypertrophy in rats with abdominal aortic constriction (Abstr.). J Mol Cell Cardiol 25 (Suppl. II): S33, 1993
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Reddy, D.S., Singh, M., Ghosh, S. et al. Role of cardiac renin-angiotensin system in the development of pressure-overload left ventricular hypertrophy in rats with abdominal aortic constriction. Mol Cell Biochem 155, 1–11 (1996). https://doi.org/10.1007/BF00714327
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DOI: https://doi.org/10.1007/BF00714327