Skip to main content
SpringerLink
Log in

Highly purified ceruloplasmin messenger RNA from rat liver

Physico-chemical and functional characteristics

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

Highly purified ceruloplasmin mRNA was isolated from rat liver polyribosomes. The molecular weight of ceruloplasmin mRNA is in a range from 1.05 to 1.25 · 106 daltons which is large enough to code for a putative precursor of ceruloplasmin (∼700 amino acids). Ceruloplasmin mRNA contains 3′-terminal poly(A) the length of which varies from 38 to 165 nucleotides. The 5′-end of ceruloplasmin mRNA is blocked with confronting m7G residue which is a component of cap I (m7G5′ppp5′XmpAp). The addition of ceruloplasmin mRNA to wheat-germ cell-free system programmed the synthesis of a product that was largely precipitated by anti-ceruloplasmin immunoglobulins. The translation product was homogeneous in polyacrylamide gel-sodium dodecylsulfate electrophoresis. Cell-free translation of ceruloplasmin mRNA was sensitive to inhibition by cap analogue.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Poulik, M. D. and Weiss, M. L. 1975. In: Plasma Proteins. Structure and Genetic Control N.-Y. Acad. Press, v.2, pp. 51–108.

    Google Scholar 

  2. Frieden, E. and Hsieh, H. S., 1976. Adv. Enzymol. 44: 187–236.

    CAS  PubMed  Google Scholar 

  3. Neifakh, S. A., Monakhov, N. K., Shaposhnikov, A. M. and Zubzitski, Ju. N., 1969. Experientia 25: 237–245.

    Article  Google Scholar 

  4. Ryden, L., 1972. Eur. J. Biochem. 26: 380–386.

    Article  CAS  PubMed  Google Scholar 

  5. Kingston, J. B., Kingston, B. L. and Putnam, F. W., 1977. Proc. Nat. Acad. Sci. USA 74: 5377–5381.

    CAS  PubMed  Google Scholar 

  6. Damashun, G., Damashun, R., Kayushina, R. L., Kreber, R., Moshkov, K. A., Müller, J., Neifakh, S. A., Pürschel, N., Shavlovski, M. M. and Belke, J., 1976. Biofizika (Russ.) 21: 965–970.

    CAS  Google Scholar 

  7. Moshkov, K. A., Lakatos, S., Hajdu, J., Zavodsky, P. and Neifakh, S. A., 1979. Eur. J. Biochem. 94: 127–134.

    Article  CAS  PubMed  Google Scholar 

  8. Neifakh, S. A., Aleynikova, T. D., Gaitskhoki, V. S., Monakhov, N. K. and Puchkova, L. V., 1979. Doklady Akademii Nauk SSSR (Russ.) 244: 238–240.

    CAS  Google Scholar 

  9. Neifakh, S. A., Vassiletz, I. M. and Shavlovski, M. M., 1972. Biochem. Genet. 6: 231–238.

    Article  CAS  PubMed  Google Scholar 

  10. Gaitskhoki, V. S., Kisselev, O. I., Moshkov, K. A., Puchkova, L. V., Shavlovski, M. M., Shulman, V. S., Vacharlovski, V. G. and Neifakh, S. A., 1975. Biochem. Genet. 13: 533–550.

    Article  CAS  PubMed  Google Scholar 

  11. Neifakh, S. A., Gaitskhoki, V. S., Klimov, N. A., Puchkova, L. V., Shavlovski, M. M. and Schwartzman, A. L. (1977). Mol. Biol. Repts 3: 235–242.

    CAS  Google Scholar 

  12. Palmiter, R. D., 1974. Biochemistry 13: 3606–3614.

    Article  CAS  PubMed  Google Scholar 

  13. Rhoads, R. E., 1975. J. Biol. Chem. 250: 8088–8097.

    CAS  PubMed  Google Scholar 

  14. Lindberg, U. and Persson, T., 1972. Eur. J. Biochem. 31: 246–253.

    Article  CAS  PubMed  Google Scholar 

  15. Gaitskhoki, V. S., L'vov., V. M., Monakhov, N. K., Puchkova, L. V., Schwartzmann, A. L., Frolova, L. Ju., Skobeleva, N. A., Zagorski, V. and Neifakh, S. A.-submitted to Eur. J. Biochem.

  16. Commerford, S. L., 1971. Biochemistry 11: 1993–2000.

    Google Scholar 

  17. Rappoport, E. and Zamecnik, P., 1975. Proc. Nat. Acad. Sci. USA 72: 313–317.

    Google Scholar 

  18. Hofer, E. and Sekeris, S. E., 1977. Biochem. and Biophys. Res. Communs 77: 352–360.

    CAS  Google Scholar 

  19. Maniatis, T., Jeffrey, A. and Sande, H., 1975. Biochemistry 14:3787–3793.

    Article  CAS  PubMed  Google Scholar 

  20. Cory, S., Genin, C. and Adams, J. M., 1976. Biochim. et Biophys. Acta 454: 248–262.

    CAS  Google Scholar 

  21. Perry, R. P. and Kelley, D. E., 1976. Cell 8: 433–442.

    Article  CAS  PubMed  Google Scholar 

  22. Randerath, E. Y. and Randerath, K., 1972. Analyt. Biochem. 48: 172–196.

    CAS  PubMed  Google Scholar 

  23. Zagorski, W., 1978. Analyt. Biochem. 87: 316–333.

    CAS  PubMed  Google Scholar 

  24. Laemmli, U. K., 1970. Nature 227: 680–685.

    Article  CAS  PubMed  Google Scholar 

  25. Shapiro, D. J., Taylor, J. M., McKnight, G. S., Palacios, R., Gonzales, C., Kiely, M. L. and Schimke, R. T., 1974. J. Biol. Chem. 249: 3665–3671.

    CAS  PubMed  Google Scholar 

  26. Schechter, I., 1974. Biochemistry 13: 1875–1885.

    Article  CAS  PubMed  Google Scholar 

  27. Taylor, J. M. and Tse, T. P. H., 1976. J. Biol. Chem. 251: 7461–7467.

    CAS  PubMed  Google Scholar 

  28. Houdebine, L. M. and Gaye, P., 1976. Eur. J. Biochem. 63: 9–14.

    Article  CAS  PubMed  Google Scholar 

  29. Miura, K., Law, S. W. T., Nishi, S. and Tamaoki, T., 1979. J. Biol. Chem. 254: 5515–5521.

    CAS  PubMed  Google Scholar 

  30. Frolova, L. Ju., Schwartzman, A. L., Skobeleva, N. A., L'vov, V. M., Gaitskhoki, V. S., Kisselev, L. L. and Neifakh, S. A., 1979. Molekularnaya Biologia (Russ.) 13: 1070–1076.

    CAS  Google Scholar 

  31. Gaitskhoki, V. S., L'vov, V. M., Schwartzman, A. L. and Neifakh, S. A., 1979. Molekularnaya Biologia (Russ.) 13: 1147–1160.

    CAS  Google Scholar 

  32. Shatkin, A. J., 1976. Cell 9: 645–653.

    Article  CAS  PubMed  Google Scholar 

  33. Hall, R. H., 1971. The Modified Nucleosides in Nucleic Acids, N.-Y., Columbia Univ. Press.

    Google Scholar 

  34. Filipowicz, W., 1978. FEBS Letters 96: 1–9.

    Article  CAS  PubMed  Google Scholar 

  35. Blobel, G. and Dobbersiein, B., 1975. J. Cell. Biol. 67: 852–862.

    CAS  PubMed  Google Scholar 

  36. Taylor, J. M., 1979. Ann. Rev. Biochem. 48: 681–717.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Biochemical Genetics, Institute for Experimental Medicine of the USSR Academy of Medical Sciences, 197022, Leningrad, USSR

    V. S. Gaitskhoki, V. M. L'vov, L. V. Puchkova, A. L. Schwartzman & S. A. Neifakh

Authors
  1. V. S. Gaitskhoki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. M. L'vov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. V. Puchkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. L. Schwartzman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. A. Neifakh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gaitskhoki, V.S., L'vov, V.M., Puchkova, L.V. et al. Highly purified ceruloplasmin messenger RNA from rat liver. Mol Cell Biochem 35, 171–182 (1981). https://doi.org/10.1007/BF02357087

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02357087

Keywords

Search

Navigation