Skip to main content
SpringerLink
Log in

Expression of stromelysin 1 in human astrocytoma cell lines

  • Laboratory Investigation
  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Summary

In a wide variety of tumor types, the expression of stromelysin 1 which is one of the matrix metalloproteinases (MMPs) has been shown to correlate with tumor invasion. However, little is known about the distribution of stromelysin in human brain tumors. We have previously shown that a correlation exists between the type IV collagenases, tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 transcripts and in vitro invasiveness among 7 human astrocytoma cell lines. In the present study, we analyzed the expression of stromelysin 1 among the same panel of human astrocytoma cell lines and human fibroblasts by northern blot analyses and in situ hybridization. Northern blot analysis demonstrated that SF-126 and U87 MG expressed high level stromelysin 1 transcripts. Following heat shock stimulation, the stromelysin 1 transcript was up-regulated in U87 MG astrocytoma cells. In situ hybridization analysis showed specific intracytoplasmic localization of mRNA for stromelysin in these astrocytoma cell lines. By casein zymography, we have determined that both SF-126 and U87 MG secreted stromelysin 1 protein. We conclude that stromelysin 1 is expressed by certain human astrocytoma cell lines, and this study confirms the importance of continuing to characterize the proteolytic enzyme profile of these tumors to fully understand the molecular mechanisms involved in astrocytoma invasiveness.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Liotta LA, Steeg PS, Stetler-Stevenson WG: Cancer metastasis and angiogenesis: An imbalance of positive and negative regulation. Cell 64: 327–336, 1991

    Google Scholar 

  2. Matrisian LM: Metalloproteinases and their inhibitors in matrix remodeling. TIG 6: 121–125, 1990

    Google Scholar 

  3. Matrisian LM: The matrix-degrading metalloproteinases. Bio Essays 14: 455–463, 1992

    Google Scholar 

  4. Apodaca G, Rutka JT, Bouhana K, Berens ME, Giblin JR, Rosenblum ML, McKerrow JH, Banda MJ: Expression of metalloproteinases and metalloproteinase inhibitor by fetal astrocytoma and glioma cells. Cancer Res 50: 2322–2329, 1990

    Google Scholar 

  5. Halaka AN, Bunning RAD, Bird CC, Gibson M, Reynolds JJ: Production of collagenase and inhibitor (TIMP) by intracranial tumors and dura in vitro. J Neurosurg 59: 461–466, 1983

    Google Scholar 

  6. Nakagawa T, Kubota T, Kabuto M, Sato K, Kawano H, Hayakawa T, Okada Y: Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors. J Neurosurg 81: 69–77, 1994

    Google Scholar 

  7. Nakano A, Tani E, Miyazaki K, Furuyama J, Matsumoto T: Expression of matrilysin and stromelysin in human glioma cells. Biochem Biophys Res Commun 192: 999–1003, 1993

    Google Scholar 

  8. Rao JS, Steck PA, Mohanam S, Stetler-Stevenson WG, Liotta LA, Sawaya R: Elevated levels of Mr 92,000 type IV collagenase in human brain tumors. Cancer Res 53: 2208–2211, 1993

    Google Scholar 

  9. Rutka JT, Hubbard SL, Fukuyama K, Matsuzawa K, Becker LE, Stetler-Stevenson W, Dirks PB: Expression of TIMP-1, TIMP-2, 72- and 92-kDa type IV collagenase transcripts in human astrocytoma cell lines: Correlation with astrocytoma cell invasiveness. Int J Oncology 6: 877–884, 1995

    Google Scholar 

  10. Chin JR, Murphy G, Werb Z: Stromelysin, a connective tissue-degrading metalloendopeptidase secreted by stimulated rabbit synovial fibroblasts in parallel with collagenase. J Biol Chem 260: 12367–12376, 1985

    Google Scholar 

  11. Saus J, Quinones S, Otani Y, Nagase H, Harris ED Jr, Kurkinen M: The complete primary structure of human matrix metalloproteinase-3. J Biol Chem 263: 6742–6745, 1988

    Google Scholar 

  12. Herron GS, Banda MJ, Clark EJ, Gavrilovic J, Werb Z: Secretion of metalloproteinases by stimulated capillary endothelial cells. J Biol Chem 261: 2814–2818, 1986

    Google Scholar 

  13. Goldberg GI, Strongin A, Collier IE, Genrich T, Marmer BL: Interaction of 92-kDa type IV collagenase with the tissue inhibitor of metalloproteinases prevents dimerization, complex formation with interstitial collagenase, and activation of the proenzyme with stromelysin. J Biol Chem 267: 4583–4591, 1992

    Google Scholar 

  14. Miyazaki K, Umenishi F, Funahashi K, Koshikawa N, Yasumitsu H, Umeda M: Activation of TIMP-2/progelatinase A complex by stromelysin. Biochem Biophys Res Commun 185: 852–859, 1992

    Google Scholar 

  15. Ogata Y, Enghild JJ, Nagase H: Matrix metalloproteinase 3 (stromelysin) activates the precursor for the human matrix metalloproteinase 9. J Biol Chem 267: 3581–3584, 1992

    Google Scholar 

  16. Suzuki K, Enghild JJ, Morodomi T, Salvesen G, Nagase H: Mechanisms of activation of tissue procollagenase by matrix metalloproteinase 3 (stromelysin). Biochemistry 29: 10261–10270, 1990

    Google Scholar 

  17. Rutka JT, Giblin JR, Hoifodt HK, Dougherty DV, Bell CW, McCulloch JR, Davis RL, Wilson CB, Rosenblum ML: Establishment and characterization of a cell line from a human gliosarcoma. Cancer Res 46: 5893–5901, 1986

    Google Scholar 

  18. Rutka JT, Giblin JR, Dougherty DV, Liu HC, McCulloch JR, Bell CW, Stem RS, Wilson CB, Rosenblum ML: Establishment and characterization of five cell lines derived from human malignant gliomas. Acta Neuropathol 75: 92–103, 1987

    Google Scholar 

  19. Carlson J, Nilsson K, Westermark B: Formation and growth of multicellular spheroids of human origin. Int J Cancer 31: 523–533, 1983

    Google Scholar 

  20. Ponten J, Macintyre EH: Long-term culture of normal and neoplastic human glia. Acta Pathol Microbiol Scand 74: 465–486, 1968

    Google Scholar 

  21. Ponten J, Westermark B: Properties of human malignant glioma cells in vitro. Medical Biology 56: 184–193, 1978

    Google Scholar 

  22. Westermark B, Ponten J, Hugosson R: Determinants for the establishment of permanent tissue culture lines from human gliomas. Acta Pathol Microbiol Scand 81: 791–805, 1973

    Google Scholar 

  23. Vance BA, Kowalski CG, Brinckerhoff CE: Heat shock of rabbit synovial fibroblasts increases expression of mRNAs for two metalloproteinases, collagenase and stromelysin. J Cell Biol 108: 2037–2043, 1989

    Google Scholar 

  24. Chomczynski P, Sacchi N: Single step method of mRNA isolation by acid guanidinium thiocyanide-phenol-chloroform extraction. Anal Biochem 162: 156–159, 1987

    Article  CAS  PubMed  Google Scholar 

  25. Declerck YA, Perez N, Shimada H, Boone TC, Langley KE, Taylor SM: Inhibition of invasion and metastasis in cells transfected with an inhibitor of metalloproteinases. Cancer Res 52: 701–708, 1992

    Google Scholar 

  26. Heussen C, Dowdle EB: Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Anal Biochem 102: 196–202, 1980

    Google Scholar 

  27. Lokeshwar BL, Selzer MG, Block NL, Gunja-Smith Z:Secretion of matrix metalloproteinases and their inhibitors (tissue inhibitor of metalloproteinases) by human prostate in explant cultures: Reduced tissue inhibitor of metalloproteinase secretion by malignant tissues. Cancer Res 53: 4493–4498, 1993

    Google Scholar 

  28. Laemmli UK: Cleavage of stractual proteins during the assembly of the head of bacteriophage T4. Nature (Loud) 227: 680–685, 1970

    Google Scholar 

  29. Frisch SM, Ruley HE: Transcription from the stromelysin promoter is induced by interleukin-1 and repressed by dexamethasone. J Biol Chem 262: 16300–16304, 1987

    Google Scholar 

  30. Quinones S, Saus J, Otani Y, Harris ED Jr, Kurkinen M: Transcriptional regulation of stromelysin. J Biol Chem 264: 8339–8344, 1989

    Google Scholar 

  31. Umenishi F, Yasumitsu H, Ashida Y, Yamauchi J, Umeda M, Miyazaki K: Purification and properties of extracellular matrix-degrading metallo-proteinase overproduced by tons sarcoma virus-transformed rat liver cell line, and its identification as transin. J Biochem 108: 537–543, 1990

    Google Scholar 

  32. Delany AM, Brinckerhoff CE: Post-transcriptional regulation of collagenase and stromelysin gene expression by epidermal growth factor and dexamethasone in cultured human fibroblasts. J Cell Biochem 50: 400–410, 1992

    Google Scholar 

  33. Kataoka H, DeCastro R, Zucker S, Biswas C: Tumor cell-derived collagenase-stimulatory factor increases expression of interstitial collagenase, stromelysin, and 72-kDa gelatinase. Cancer Res 53: 3154–3158, 1993

    Google Scholar 

  34. Brinckerhoff CE, McMillan RM, Fahey JV, Harris ED Jr: Collagenase production by synovial cells treated with phorbol myristate acetate. Arthritis Rheum 22: 1109–1116, 1979

    Google Scholar 

  35. Matrisian KM, McDonnell S, Miller DB, Navre M, Seftor Ba EA, Hendrix MJC: The role of the matrix metalloproteinase stromelysin in the progression of squamous cell carcinoma. Am J Med Sci 302: 157–162, 1991

    Google Scholar 

  36. Ostrowski LE, Finch J, Krieg P, Matrisian L, Patskan G, O'Connell JF, Phillips J, Slaga TJ, Breathnach R, Bowden GT. Expression pattern of a gene for a secreted metalloproteinase during late stages of tumor progression. Mol Carcinogenesis 1: 13–19, 1988

    Google Scholar 

  37. Sreenath T, Matrisian LM, Stetler-Stevenson W, Gattoni-Celli S, Pozzatti RO: Expression of matrix metalloproteinase gene in transformed rat cell lines of high and low metastatic potential. Cancer Res 52: 4942–4947, 1992

    Google Scholar 

  38. Albini A, Melchiori A, Santi L, Liotta LA, Brown PD, Stetler-Stevenson WG: Tumor cell invasion inhibited by TIMP-2. J Natl Cancer Inst 83: 775–779, 1991

    Google Scholar 

  39. Yang TT, Hawkes SP: Role of the 21-kDa protein TIMP-3 in oncogenic transformation of cultured chicken embryo fibroblasts. Proc Natl Acad Sci USA 89: 10676–10680, 1992

    Google Scholar 

  40. Matrisian LM, Bowden GT, Krieg P, Furstenberger G, Briand J-P, Leroy P, Breathnach R: The mRNA coding for the secreted protease transin is expressed more abundantly in malignant than in benign tumors. Proc Natl Acad Sci USA 83: 9413–9417, 1986

    Google Scholar 

  41. Sato H, Kida Y, Mai M, Endo Y, Sasaki T, Tanaka J, Seiki M: Expression of gene encoding type IV collagen-degrading metalloproteinases in various human tumor cells. Oncogene 7: 77–83, 1992

    Google Scholar 

  42. Wright J, McDonnell S, Matrisian LM: Stromelysin-1 is expressed in stromal cells as well as keratinocytes in the mouse skin model of two-stages carcinogenesis. Proc AACR 34: 174, 1993

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Brain Tumor Research Laboratory, Division of Neurosurgery, and Division of Neuropathology (LEB), Hospital for Sick Children, University of Toronto, Ontario, Canada

    Kazuhito Matsuzawa, Kouzou Fukuyama, Peter B. Dirks, SherriLynn Hubbard, Masaji Murakami, Laurence E. Becker & James T. Rutka

Authors
  1. Kazuhito Matsuzawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kouzou Fukuyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter B. Dirks
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. SherriLynn Hubbard
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masaji Murakami
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Laurence E. Becker
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. James T. Rutka
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsuzawa, K., Fukuyama, K., Dirks, P.B. et al. Expression of stromelysin 1 in human astrocytoma cell lines. J Neuro-Oncol 30, 181–188 (1996). https://doi.org/10.1007/BF00177269

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00177269

Key words

Search

Navigation