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Studies on the submicrosomal fractions of bovine oligodendroglia: Lipid composition and glycolipid biosynthesis

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Abstract

Oligodendroglia were isolated from bovine brain, and a “crude”, microsomal fraction obtained from cell homogenates was subfractionated into myelin (MP), plasma membranes (PM), Golgi (GF), smooth (SER) and rough (RER) endoplasmic membranes using discontinuous-sucrose gradient centrifugation. The submicrosomal fractions were characterized by ultrastructural examination and analysis of the specific organelle markers. The myelin and plasma membrane rich fractions contained characteristically the highest amounts of the lipid with lower mole percentages of total phospholipids and phosphatidylcholine, and higher concentrations of phosphatidylethanolamine (+plasmalogens), cholesterol and galactolipids. Considerable amounts of the typical myelin galactolipids (galacto-cerebrosides, sulfatides and monogalactosyl diglycerides) were also found in the Golgi fraction (GF). The GF fraction had the greatest enrichment of glycolipid-forming galactosyltransferases, and the distribution of these enzymes correlated well with that of the Golgi marker enzymes. The results give evidence that intracellular Golgi apparatus of oligodendroglia is rich in the myelin-specific lipids, and suggest its involvement in the synthesis and processing of myelin lipids.

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Authors and Affiliations

  1. Department of Neurochemistry, Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY

    D. S. Deshmukh, P. K. Lee, W. D. Bear & S. Kuizon

  2. Department of Pathological Neurobiology, Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY

    A. W. Vorbrodt

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Deshmukh, D.S., Vorbrodt, A.W., Lee, P.K. et al. Studies on the submicrosomal fractions of bovine oligodendroglia: Lipid composition and glycolipid biosynthesis. Neurochem Res 13, 571–582 (1988). https://doi.org/10.1007/BF00973300

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