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Ethanol-Induced Cell Death by Lipid Peroxidation in PC12 Cells

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Abstract

Free radical generation is hypothesized to be the cause of alcohol-induced tissue injury. Using fluorescent cis-parinaric acid and TBARS, lipid peroxidation was shown to be increased in the presence of trace amounts of free ferrous ion in PC12 cells. This increase in lipid peroxidation was enhanced by ethanol in a dose dependent manner and also correlated with loss of cell viability, as measured by increased release of lactate dehydrogenase (LDH). Resveratrol, a potent antioxidant, had a protective effect against lipid peroxidation and cell death. These findings strongly suggest that ethanol-induced tissue injury and cell death is a free radical mediated process, and may be important in alcohol-related premature aging and other degenerative diseases.

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Author notes

  1. Yu Cheng

    Present address: Department of Neurology, Washington University, St. Louis, Mo, 63110

Authors and Affiliations

  1. Department of Pharmacology, University of Missouri, Columbia, MO, 65212

    Albert Y. Sun, Yong-mei Chen, Marilyn James-Kracke & Patricia Wixom

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Sun, A.Y., Chen, Ym., James-Kracke, M. et al. Ethanol-Induced Cell Death by Lipid Peroxidation in PC12 Cells. Neurochem Res 22, 1187–1192 (1997). https://doi.org/10.1023/A:1021968526696

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