Abstract
Our research group has been investigating the effect of cyclic deformations on the evolution of fibroblast populated collagen gels (FPCG). Since existing traction machines are not designed for such an application, we had to design a cyclic traction machine adapted to tissue culture inside an incubator over an extended period of time. Biocompatible materials were used for fabrication to allow for easy sterilization and to prevent any adverse reaction from the tissue. The traction machine is based on a computer-controlled stepping motor system for easy adjustment of the deformation amplitude and frequency. The maximum stretching speed achieved is around 1mm/s. The traction machine can measure FPCG mechanical properties and perform rupture tests to determine its ultimate strength. Several FPCGs have been successfully cultured with the machine for up to four weeks without any adverse reaction. © 1999 Biomedical Engineering Society.
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REFERENCES
Buck, R. C. Reorientation response of cells to repeated stretch and recoil of the substratum, Exp. Cell Res. 127:470–474, 1980.
Dartsch, P. C., J. Hämmerle, and E. Betz. Orientation of cultured arterial smooth muscle cells growing on cyclically stretched substrates. Acta Anat. 125:108–113, 1986.
Goulet, F., L. Germain, D. Rancourt, C. Caron, A. Normand, and F. A. Auger. Tendons and ligaments. In: Textbook of Tissue Engineering, edited by R. Lanza, R. Langer, and W. L. Chick. Austin, TX: Landes RG Co. and Academic, 1996, pp. 629–640.
Goulet, F., L. Germain, C. Caron, D. Rancourt, A. Normand, and F. A. Auger. Tissue engineered ligament. In: Ligaments & Ligamentoplasties, edited by L. H. Yahia. New York: Springer, 1996, pp. 367–377.
Huang, D., T. R. Chang, A. Aggarwal, R. C. Lee, and H. P. Ehrlich. Mechanisms and dynamics of mechanical strengthening of ligament-equivalent fibroblast-populated collagen matrices. Ann. Biomed. Eng. 21:289–305, 1993.
Ives, C. L., S. G. Eskin, and L. V. McIntire. Mechanical effects on endothelial cell morphology: in vitro assessment. In Vitro Cell. Dev. Biol.: Anim. 22(9):500–507, 1986.
Kanda, K., and T. Matsuda. In vitro reconstruction of hybrid arterial media with molecular and cellular orientations. Cell Transplant. 3(6):537–545, 1994.
Kanda, K., and T. Matsuda. Mechanical stress-induced orientation and ultrastructural change of smooth muscle cells cultured in three-dimensional collagen lattices. Cell Transplant. 3(6):481–492, 1994.
Kanda, K., T. Matsuda, and T. Oka. Mechanical stressinduced cellular orientation and phenotypic modulation of 3-D cultured smooth muscle cells. ASAIO J. 39(3):M686-M690, 1993.
Kanda, K., T. Matsuda, and T. Oka. Two-dimensional orientational response of smooth muscle cells to cyclic stretching. ASAIO J. 38:M382-M385, 1992.
Labelle, S. Conception d'un appareil pour l'étude in vitro de la réparation ligamentaire sous stimulation mécanique. Mechanical Engineering Department, École Polytechnique de Montréal: Master thesis, 1988.
Leung, D. Y. M., S. Glagov, and M. B. Mathews. A new in vitro system for studying cell response to mechanical stimulation. Exp. Cell Res. 109:285–298, 1977.
Light, N. D., and A. J. Bailey. Covalent cross-links in collagen. Methods Enzymol. 82:360, 1982.
López Valle, C. A., F. A. Auger, P. Rompré, V. Bouvard, and L. Germain. Peripheral anchorage of dermal equivalents. Br. J. Dermatol. 127:365–371, 1992.
Shirinsky, V. P., A. S. Antonov, K. G. Birukov, A. V. Sobolevsky, Y. A. Romanov, N. V. Kabaeva, G. N. Antonova, and V. N. Smirnov. Mechano-chemical control of human endothelium orientation and size. J. Cell Biol. 109:331–339, 1989.
Yahia, L. H., E. A. Desrosiers, and C. H. Rivard. A computer-controlled apparatus for in vitro mechanical stimulation and characterization of ligaments. Bio-Med. Mater. Eng. 1(4):215–222, 1991.
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Langelier, E., Rancourt, D., Bouchard, S. et al. Cyclic Traction Machine for Long-Term Culture of Fibroblast-Populated Collagen Gels. Annals of Biomedical Engineering 27, 67–72 (1999). https://doi.org/10.1114/1.166
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DOI: https://doi.org/10.1114/1.166