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Investigations on some constituents of Lupinus termis seeds

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Abstract

An investigation of the composition of two varieties ofLupinus termis seeds showed the presence of proteins, free amino acids, lipids, lignin, polysaccharides and mannitol. The polysaccharide materials (27.47 – 34.33%) consisted of starch, combined galacturonic acid, galactose, arabinose, xylose and rhamnose. Combined galactose and rhamnose comprised the major components of polysaccharide materials. Protein's constituted 46.11 – 50.57% ofL. termis seeds. The amino acid composition of the seeds as well as those of the proteins isolated with different agents were determined. The amino acid patterns of free and combined amino acids varied according to the extracting agent.

Résumé

Une recherche de la composition des deux variétés des grains duLupinus termis montre la presence des protéines, acides aminés libres, lipides, lignin, polysaccharides et le mannitol. Les polysaccharides (24.47 – 34.33%) se composent de starch, acide galacturonique combiné, galactose, arabinose, xylose et rhamnose. Galactose et rhamnose combinés forment la majorité de la constitution des materiels polysaccharides. Les protéines ont formé 46.11 – 50.57% de grains duLupinus termis. La composition des acides aminés des grains, aussi bien que celle des protéines isolés par plusieurs agents ont été déterminés. Les cadres d'acide aminé des acides libres et combinés ont été variés selon l'emploi de l'agent d'extraction.

Zusammenfassung

Eine Untersuchung der Samen zweierLupinus termis Sorten ergab die Anwesenheit von Proteinen, freien Aminosäuren, Lipiden, Lignin, Polysacchariden und Mannit. Die Polysaccharid-Fraktion (27,47 – 34,33%) besteht aus Stärke, gebundener Galakturonsäure, Galaktose, Arabinose, Xylose und Rhamnose. Die gebundene Galaktose und Rhamnose bilden die Hauptbestandteile der Polysaccharide. Die Proteine machen 46,11 – 50,57% derL. termis Samen aus.

Es wurde sowohl die Aminosäurenzusammensetzung der Samen bestimmt als auch die der mit verschiedenen Agentien isolierten Proteine. Die Aminosäurenmuster der freien und gebundenen Aminosäuren variieren je nach dem Extraktionsmittel.

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References

  • Abdel-Fattah, A. F. & Edrees, M. (1971). Chemical Investigations on Some Constituents of Pigmented Onion Skins.J. Sci. Fd Agric. 22:298–300.

    Google Scholar 

  • Abdel-Fattah, A. F. & Hussein, M. M. (1970). Composition of Some Brown Algae as Influenced by Seasonal Variation.Phytochem. 9:721–724.

    Google Scholar 

  • Bitter, T. & Muir, H. M. (1962). A Modified Uronic Acid Carbazole Reaction.Analyt. Biochem. 4:330–334.

    PubMed  Google Scholar 

  • Block, R. J., Durrum, E. L. & Zweig, U. (1955). A Manual of Paper Chromatography and Paper Electrophoresis. Academic Press, New York: 127–156.

    Google Scholar 

  • Bourne, E. J., Johnson, P. G. & Percival, E. (1970). The Water Soluble Polysaccharides ofCladophora rupestris. Part IV. Autohydrolysis, Methylation of the Partially Desulphated Material, and Correlation with the Results from Smith Degradation.J. Chem. Soc. (C):1561–1569.

  • Brown, A. H. (1946). Determination of Pentose in the Presence of Large Quantities of Glucose.Arch. Biochem. 11:269–278.

    Google Scholar 

  • Dedonder, R. (1952). Glucides et Composés Voisins.Bull. Soc. Chim. France: 874–883.

  • Dische, Z. & Shettles, L. B. (1948). A Specific Color Reaction of Methyl Pentoses and a Spectrophotometric Micromethod for their Determination.J. Biol. Chem. 175:595–603.

    Google Scholar 

  • Dische, Z., Shettles, L. B. & Osnos, M. (1949). New Specific Color Reactions of Hexoses and Spectrophotometric Micromethods for their Determination.Arch. Biochem. 22:169–184.

    Google Scholar 

  • Giri, K. V., Radhakrishnan, A. N. & Vaidyanathan, C. S. (1952). Some Factors Influencing the Quantitative Determination of Amino Acids Separated by Circular Paper Chromatography.Anal. Chem. 24:1677–1678.

    Google Scholar 

  • Hirst, E. L., Jones, J. K. N. & Walder, W. O. (1947). Pectic Substances. VII. The Constitution of the Galactans fromLupinus albus. J. Chem. Soc.: 1225–1229.

  • Partridge, S. M. (1948). Filter — paper Partition Chromatography of Sugars. I. General Description and Application to the Qualitative Analysis of Sugars in Apple Juice, Egg White and Foetal Blood of Sheep.Biochem. J. 42:238–250.

    Google Scholar 

  • Selim, A. S. M., El-Hawari, Z. & Hassan, A. (1966). The Amino Acid Pattern ofLupinus termis andCyperus esculentus.J. Chem. U.A.R. 9:117–123.

    Google Scholar 

  • Slotto, K. H. & Primosigh, J. (1951). Amino Acid Composition of Crotoxin.Nature 168:696–697.

    PubMed  Google Scholar 

  • Tadros, W. & Kamel, M. (1952). A Preliminary Study of the Main Polysaccharide ofLupinus termis Seeds.J. Chem. Soc.: 4532–4533.

  • Tomoda, M. & Murayama, K. (1965). The Polysaccharide fromLupinus luteus Seeds. I. Identification of 2, 3, 6-tri-O-methyl-D-Galactose from the Methylated Substance.Yakugaku Zasshi 85:511–514; Cited fromC.A. (1965) 63:6020.

    PubMed  Google Scholar 

  • Whistler, R. L. (1965). Methods in Carbohydrate Chemistry, Academic Press, New York and London, Vol. 5: 185–187.

    Google Scholar 

  • Zucas, S. M., Barberio, J. C. & Orlandi, M. M. G. (1961). Nutritive Value of Thirty Edible Vegetables from Brazil. I. Percentage Composition.Anais Farm. Quim. Sao Paulo 12:155–163 Cited fromC.A. (1962) 57:10311.

    Google Scholar 

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Abdel-Fattah, A.F., Zaki, D.A., Edrees, M. et al. Investigations on some constituents of Lupinus termis seeds. Plant Food Hum Nutr 23, 359–368 (1974). https://doi.org/10.1007/BF01095423

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