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Assignment of mouse beta-spectrin gene to chromosome 12

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Somatic Cell and Molecular Genetics

Abstract

The structural gene for the Β-subunit of the mouse erythrocyte spectrin, hereinafter designated as Sp-b, was assigned to the mouse chromosome 12. This assignment was made by Southern analysis of genomic DNA from mouse X Chinese hamster hybrid cells using cloned mouse erythrocyte Β-spectrin cDNA as a probe. In the PstI-digested genomic hamster cell DNA a single band of 2.0 kb was detected, whereas PstI-digested mouse DNA gave a band of 4.2 kb, when probed with the mouse erythroid Β-spectrin cDNA clone. This allowed us to analyze a panel of mouse X Chinese hamster somatic cell hybrids to map this gene to chromosome 12. Interestingly, this assignment is different from that observed for the α-subunit of spectrin, which has been mapped to chromosome 1 in mouse. These results serve as a basis for further genetic characterization of the mouse hemolytic anemias.

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Author notes

  1. Livia Cioe

    Present address: Institute of Virology, University of Rome, 00185, Rome, Italy

Authors and Affiliations

  1. The Wistar Institute of Anatomy and Biology, 36 and Spruce Streets, 19104, Philadelphia, Pennsylvania

    Pekka Laurila, Livia Cioe & Peter J. Curtis

  2. Laboratory of Molecular Biology, National Institute of Allergy and Infectious Diseases, 20892, Bethesda, Maryland

    Christine A. Kozak

Authors
  1. Pekka Laurila
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  2. Livia Cioe
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  3. Christine A. Kozak
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  4. Peter J. Curtis
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Laurila, P., Cioe, L., Kozak, C.A. et al. Assignment of mouse beta-spectrin gene to chromosome 12. Somat Cell Mol Genet 13, 93–97 (1987). https://doi.org/10.1007/BF02422304

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  • DOI: https://doi.org/10.1007/BF02422304

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