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Papierchromatographische Trennung und quantitative Bestimmungsmethode für SH-Glutathion in Blut und Geweben

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Zusammenfassung

Beschreibung eines Trennverfahrens mittels horizontaler Papierchromatographie für reduziertes Glutathion in Blut und Geweben und dessen quantitative Bestimmung.

1. Nach vorheriger Stabilisierung des GSH durch N-Äthylmaleinimid im Bluthämolysat oder Gewebshomogenat wird eine oxydative Veränderung zu GSSG während aller weiteren Aufarbeitungsvorgänge verhindert.

2. Die Empfindlichkeit der Ninhydrin-Cadmium-Reaktion gestattet es, GSH-Mengen bis 0,2 µg quantitativ-colorimetrisch zu erfassen. Der chromatographische GSH-Nachweis ist, auch auf Grund seiner Spezifität, allen bisherigen Bestimmungsverfahren überlegen.

Summary

A method for horizontal paper chromatographic separation and quantitative estimation of reduced glutathione (GSH) in blood and tissues is described. GSH is primarily stabilized by N-ethylmaleimide in the haemolysate or tissue homogenate to prevent oxidation during all further procedures. By the ninhydrin-cadmium dip-reagent GSH-concentrations to 0,2 µg can be exactly estimated.

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Authors and Affiliations

  1. Aus der Medizinischen Universitätsklinik Würzburg, Germany

    H. Wernze & W. Koch

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Wernze, H., Koch, W. Papierchromatographische Trennung und quantitative Bestimmungsmethode für SH-Glutathion in Blut und Geweben. Klin Wochenschr 43, 454–459 (1965). https://doi.org/10.1007/BF01483854

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