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Präparation eines Antiserums gegen enzymatisch gespaltenes Gammaglobulin (Gammavenin®)

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Zusammenfassung

Durch Sensibilisierung von Kaninchen mit pepsinverdautem menschlichen Gammaglobulin (Gammavenin®) wurde ein Serum gewonnen, das relativ kräftige Präcipitine gegen die Receptoren der H- und der L- (Lambda- und Kappa-)Polypeptidketten des Immunglobulin IgG enthält. In der immunelektrophoretischen Analyse kommen bei Verwendung dieses Antiserums im IgG-Bereich drei Linien zur Darstellung, die den präzipitierten H- und L-Ketten des komplettenIg G-Moleküls entsprechen. Es wird demonstriert, daß die nach der beschriebenen Methode gewonnenen Antiseren für die immunelektrophoretische Analyse von Paraproteinen erhebliche Vorteile besitzen.

Summary

A serum was obtained by sensibilisation of rabbits with pepsin digested human gamma-globulin (Gammavenin®), which contained relatively strong precipitants against the receptors of the h-chain and l-chains (kappa and lambda) of the immunoglobulin IgG. In the immunoelectrophoretic analysis three lines in the IgG-area can thus be demonstrated, which correspond to the h- and l-polypeptid-chains of the complete IgG-molecule. It has been shown, that those antisera are dvantageous for the immunoelectrophoretic analysis of paraproteins.

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Literatur

  1. Cohen, S., andR. R. Porter: Heterogenity of the peptide chains of gamma-globulin. Biochem. J.90, 278 (1964).

    Google Scholar 

  2. Cohen, S., andR. R. Porter: Structure and biological activity of immunoglobulins. Advanc. Immunol.4, 287 (1964).

    Google Scholar 

  3. Edelman, G. M., J. F. Heremans, M. T. Heremans, andH. G. Kunkel: Immunological studies of human gamma-globulin. J. exp. Med.112, 203 (1960).

    Google Scholar 

  4. Edelman, G. M., andM. D. Poulik: Studies on structural units of the gamma-globulins. J. exp. Med.113, 861 (1961).

    Google Scholar 

  5. Edelman, G. M., andB. Benacerraf: On structural and functional relations between antibodies and proteins of the gamma-system. Proc. nat. Acad. Sci. (Wash.)48, 1035 (1962).

    Google Scholar 

  6. Fahey, J. L.: Two types of 6,6 S gamma-globulins, beta-2a-globulins and 18 S gamma 1-macroglobulins in normal serum and gamma-microglobulins in normal urine. J. Immunol.91, 438 (1963).

    Google Scholar 

  7. Fahey, J. L.: Structural basis for the differences between type I and type II human gamma-globulin molecules. J. Immunol.91, 448 (1963).

    Google Scholar 

  8. Fahey, J. L., andC. McLaughin: Preparation of antisera specific for 6,6 S gamma-globulins, gamma 1-macroglobulins, beta 2A-globulins, and for type I and II common gamma-globulin determinants. J. Immunol.91, 484 (1963).

    Google Scholar 

  9. Fleischman, J. B., R. H. Pain, andR. R. Porter: Reduction of the gamma-globulins. Arch. Biochem. Suppl.1, 174 (1962).

    Google Scholar 

  10. Franklin, E. C., andH. G. Kunkel: Comparative levels of high molecular weight (19 S) gammaglobulin in maternal and umbilical cord serum. J. Lab. clin. Med.52, 724 (1958).

    Google Scholar 

  11. Franklin, E. C., andD. R. Stanworth: Antigenic relationships between immune globulins and certain related paraproteins in man. J. exp. Med.114, 521 (1961).

    Google Scholar 

  12. Franklin, E. C., H. Fudenberg, M. Meltzer, andD. R. Stanworth: The structural basis for genetic variations of normal human gamma-Globulins. Proc. nat. Acad. Sci. (Wash.)48, 914 (1962).

    Google Scholar 

  13. Heremans, J. F.: Les globulines seriques du systeme gamma. (ed. Arscia, S. A.). Brüssel 1960.

  14. Korngold, L., andR. Lipari: Multiple-myeloma proteins; immunological studies. Cancer (Philad.)9, 183 (1956).

    Google Scholar 

  15. Mannik, M., andH. G. Kunkel: Two major types of normal 7 S gamma-globulin. J. exp. Med.117, 213 (1963).

    Google Scholar 

  16. Migita, S., andF. W. Putnam: Antigenic relationships of Bence-Jones-proteins, myeloma globulins, and normal human gamma-globulin. J. exp. Med.117, 81 (1963).

    Google Scholar 

  17. Nisonoff, A., F. C. Wissler, L. N. Lipman, andD. L. Woernly: Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch. Biochem.89, 230 (1960).

    Google Scholar 

  18. :Nomenclature for human immunoglobulins. Int. Arch. Allergy25, 242 (1964).

    Google Scholar 

  19. Nussenzweig, V., E. C. Franklin, andB. Benacerraf: Immunologic properties of products by mild reduction of human and guinea pig gammaglobulins. J. Immunol.93, 1015 (1964).

    Google Scholar 

  20. Porter, R. R., andE. M. Press: Immunochemistry. Ann. Rev. Biochem.31, 625 (1962).

    Google Scholar 

  21. Scheidegger, J. J.: Une micromethode de l'immunoelectrophorese. Int. Arch. Allergy7, 103 (1955).

    Google Scholar 

  22. Schultze, H. E., u.G. Schwick: Über neue Möglichkeiten intravenöser Gammaglobulin-Applikation. Dtsch. med. Wschr.87, 1643 (1962).

    Google Scholar 

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Authors and Affiliations

  1. Abteilung für klinische Immunpathologie, Universitäts-Kinderklinik Hamburg-Eppendorf, Germany

    F. Bläker & K. Fischer

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  1. F. Bläker
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  2. K. Fischer
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Mit Unterstützung der Deutschen Forschungsgemeinschaft.

Mit technischer Assistenz vonI. Lichtenstein undI. Fritz.

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Bläker, F., Fischer, K. Präparation eines Antiserums gegen enzymatisch gespaltenes Gammaglobulin (Gammavenin®). Klin Wochenschr 45, 494–495 (1967). https://doi.org/10.1007/BF01717439

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  • DOI: https://doi.org/10.1007/BF01717439

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