Summary
Methods are presented which provide the preparation of highly labelled nuclear RNA from cells of the different forms of human leukaemia. An incubation medium is described that is based on Hepes buffer and on the use of exhaustively dialyzed fetal calf serum offering suitable conditions for the uptake of32P-orthophosphate into the RNA of leukaemic nuclei. The specific activities reached may allow more detailed nucleotide and oligonucleotide analyses of the various RNA species present in human leukaemic cells.
Zusammenfassung
Es werden Methoden zur Präparation von nuklearer RNA hoher spezifischer Aktivität aus menschlichen Leukämiezellen beschrieben. Das Inkubationsmedium basiert auf Hepes-Puffer und der Verwendung von dialysiertem Kalbsserum zur Verbesserung der Bedingungen für den32P-Orthophosphat-Einbau in die hochmolekulare Kern-RNA. Die erreichten spezifischen Aktivitäten erlauben detaillierte Nucleotid- und Oligonucleotidanalysen der verschiedenartigen Ribonucleinsäurespezies in menschlichen Leukämiezellen.
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Mit Unterstützung durch das Landesamt für Forschung, Nordrhein-Westfalen.
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Seeber, S., Schmidt, C.G. Isolation of rapidly labelled nuclear RNA of high specific activity from human leukaemic cells. Klin Wochenschr 51, 677–679 (1973). https://doi.org/10.1007/BF01468172
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DOI: https://doi.org/10.1007/BF01468172