Summary
By means of differential centrifugation on top of a Biligram®-Dextran mixture, more than 99% of the erythrocytes and granulocytes and more than 85% of the lymphocytes were removed from a 30 ml blood sample. The further separation of the monocytes from the lymphocytes and thrombocytes was accomplished by differential sedimentation and adherence on to a plastic surface. According to the morphological and functional characteristics the isolated cells showed to be monocytes or mononuclear phagocytes, respectively. As yet the method was employed for normal blood and blood of patients with tuberculosis.
Zusammenfassung
Durch Differential-Zentrifugierung über einem Biligram®-Dextran-Gemisch wurden über 99% der Erythrocyten und Granulocyten und über 85% der Lymphocyten aus einer 30 ml-Blutprobe entfernt. Die weitere Abtrennung der Monocyten von den Lymphocyten und Thrombocyten erfolgte durch Differential-Sedimentierung und Adhärenz an einer Plastik-Oberfläche. Bei den isolierten Zellen handelte es sich nach morphologischen und funktionellen Kriterien um Monocyten bzw. mononucleäre Phagocyten. Verwendet wurde bisher Normalblut und Blut Tuberkulose-Kranker.
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Urbanitz, D., Gross, R. Einfache Methode zur Isolierung von Monocyten aus dem Blut. Klin Wochenschr 52, 99–101 (1974). https://doi.org/10.1007/BF01468333
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DOI: https://doi.org/10.1007/BF01468333