Skip to main content
SpringerLink
Log in

Cytoplasmic DNA synthesis by cord blood cells of premature and full-term infants

DNS-Synthese in Cytoplasma von Nabelschnurblutzellen bei Frühgeborenen und reifen Neugeborenen

An autoradiographic study

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Zusammenfassung

Leukocyten aus Nabelschnurblut von 8 reifen und 12 unreifen Neugeborenen und Blutleukocyten von 10 Erwachsenen wurden mit3H-Thymidin inkubiert. Die anschließende Belichtung der autoradiographischen Filmemulsion erfolgte für 45 und 90 Tage. 1,7% der Lymphoidzellen im Erwachsenenblut, 2,9% und 4,5% der Lymphoidzellen im Nabelschnurblut von Reifgeborenen bzw. Frühgeborenen zeigten eine cytoplasmatische Markierung, die als Ausdruck mitochondraler DNS-Synthese gewertet wurde. Die statistische Auswertung zeigt, daß markierte Zellen im Blut der Frühgeborenen signifikant (p<0,01) häufiger sind als im Blut von Erwachsenen.

Summary

Cord blood cells from 8 full-term and 12 premature infants as well as blood leukocytes from 10 adults were incubated with3H-thymidine and were subsequently exposed to autoradiographic emulsion for prolonged periods of 45 and 90 days. Cytoplasmic label — which we interpreted as evidence of mitochondrial DNA synthesis — was found in 1.7 per cent of lymphoid cells from adult blood, in 2.9 per cent and 4.5 per cent of lymphoid cells from full-term and premature infants, respectively. Statistical analysis of the data shows that the number of labelled cells is significantly larger in the blood of premature infants than in adults (p<0.01).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Baugnet-Mahieu, L., Goutier, R.: Reutilization of labelled-thymidine and -iododeoxyuridine for nuclear and mitochondrial DNA synthesis in regenerating rat liver. Arch. Internat. Physiol. Biochim.80, 319 (1972)

    Google Scholar 

  2. Bell, E.: Informational DNA synthesis distinguished from that of nuclear DNA by inhibitors of DNA synthesis. Science174, 603 (1971)

    PubMed  Google Scholar 

  3. Borst, P., Kroon, A.M.: Mitochondrial DNA: Physiochemical properties, replication, and genetic function. Int. Rev. Cytol.26, 107 (1969)

    PubMed  Google Scholar 

  4. Chang, L.O., Looney, W.B.: Incorporation of tritiated thymidine into mitochondrial and nuclear DNA in normal and regenerating rat liver before and after irradiation. Int. J. Rad. Biol.12, 187 (1966)

    Google Scholar 

  5. Dobson, R.L., Cooper, M.F.: Incorporation of radioactivity from thymidine into mammalian glucose and glycogen. Biochim. biophys. Acta254, 393 (1971)

    PubMed  Google Scholar 

  6. Efrati, P., Rosenszajn, L., Shapira, E.: Blood of normal human newborns. The morphology of buffy coat from cord blood. Blood17, 497 (1961)

    PubMed  Google Scholar 

  7. Faulk, W.P., Goldman, J.R., Maloney, M.A., Fudenberg, H.H., Yoffey, J.M.: Morphology and nucleoside incorporation of human neonatal lymphocytes. Cell. Immunology8, 166 (1973)

    Google Scholar 

  8. Feinendegen, L.E.: Tritium-Labeled Molecules in Biology and Medicine. Monographic Series on Radiation Biology, p. 242 Acad. Press New York and London, 1967

    Google Scholar 

  9. Friedkin, M., Tilson, D., De Roberts, W.: Studies of deoxyribonucleic acid bio synthesis in embryonic tissues with thymidine C14. J. biol. Chem.220, 627 (1965)

    Google Scholar 

  10. Garmick, S.G., Gibor, A.: The DNA of chloroplasts, mitochondria, and centrioles. Progr. Nucl. Acid Res. Molec. Biol.6, 143 (1967)

    Google Scholar 

  11. Gross, N.J., Getz, G.S., Rabinowitz, M.: Turnover of mitochondrial DNA and phospholipid in rat tissues. In: Biochemical Aspects of the Biogenesis of Mitochondria. E.C. Slater, J.N. Tager, S. Papa, and E. Quagliariello (Eds), p. 161. Bari: Adriatica Editrice (1968)

    Google Scholar 

  12. Hall, M.R., Meinecke, W., Goldstein, D.A., Lerner, R.A.: Synthesis of cytoplasmic membrane — associated DNA in lymphocyte nucleus. Nature (New Biol.)234, 227 (1971)

    PubMed  Google Scholar 

  13. Kalf, G.F., Agostino, M.A., Hunter, G.R.: DNA biosynthesis by isolated mitochondria stimulation by cytoplasmic factors from neoplastic and regenerating tissues. Cancer Res.31, 2054 (1971)

    PubMed  Google Scholar 

  14. Koch, J.: Cytoplasmische DNAs in Animalzellen; das Problem der Existenz einer „Messenger-DNA“. Hoppe Seyler's Z. Physiol. Chem.353, 727 (1972)

    Google Scholar 

  15. Koch, J., Pfeil, H.V.: Transport of nuclear DNA into the cytoplasm in cultured animal cells. A survey. FEBBS Letters24, 53 (1972)

    Google Scholar 

  16. Koch, J., Stockstad, E.L.R.: Incorporation of (3H) thymidine into nuclear and mitochondrial DNA in synchronized mammalian cells. Europ. J. Biochem.3, 11 (1967)

    PubMed  Google Scholar 

  17. Morley, C.G.D., Kingdon, H.S.: Use of3H-thymidine for measurement of DNA synthesis in rat liver — a warning. Analyt. Biochem.45, 298 (1972)

    PubMed  Google Scholar 

  18. Nass, S.: Incorporation of32Pi into mitochondrial and nuclear DNA in regenerating liver. Biochim. biophys. Acta145, 660 (1967)

    Google Scholar 

  19. Neubert, D., Oberdisse, E., Bass, R.: Biosynthesis and degradation of mammalian mitochondrial DNA. In: Biochemical Aspects of the Biogenesis of Mitochondria, E.C. Slater, J.M. Tager, S. Papa, and E. Quagliariello (Eds), p. 103. Bari: Adriatica Editrice 1968

    Google Scholar 

  20. Parson, P., Simpson, M.V.: Deoxyribonucleic acid biosynthesis and mitochondria. Studies on the incorporation of labelled precursors into mitochondrial deoxyribonucleic acid. J. biol. Chem.248, 1912 (1973)

    PubMed  Google Scholar 

  21. Prindull, G.: Spontaneous DNA synthesis of blood lymphoid cells in premature newborn infants, in older premature infants, and in full-term newborn infants. Z. Kinderheilk.118, 197 (1974)

    PubMed  Google Scholar 

  22. Prindull, G., Prindull, B., Ron, A., Yoffey, J.M.: Cells in spontaneous DNA synthesis in cord blood of premature and fullterm newborn infants. An autoradiographic study. J. Pediat.86, 773 (1975)

    PubMed  Google Scholar 

  23. Schneider, W.C., Kuff, E.L.: The isolation and some properties of rat liver mitochondrial deoxyribonucleic acid. Proc. nat. Acad. Sci. (Wash.)54, 1650 (1965)

    PubMed  Google Scholar 

  24. Winter, G.C.B., Byles, A.B., Yoffey, J.M.: Blood lymphocytes in newborn and adult. The Lancet2, 932 (1965)

    Google Scholar 

  25. Yoffey, J.M.: Stem cell role of the lymphocyte transitional (CT) compartment. In: Haemopoietic Stem Cells, G.E.W. Wolstenholme and M. O'Conner (Eds), p. 5. Ciba Symposium, Elsevier, Excerpta Medica, North Holland, Assoc. Sci. Publ.: Amsterdam, London and New York: 1973

    Google Scholar 

  26. Yoffey, J.M.: Bone Marrow in Hypoxia and Rebound. Chas. C. Thomas Co., Springfield, Illinois: 1974

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Anatomy and Hematology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel

    G. Prindull, A. Ron & J. M. Yoffey

  2. Universitäts-Kinderklinik, Humboldtallee 38, D-3400, Göttingen, Bundesrepublik Deutschland

    G. Prindull

Authors
  1. G. Prindull
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Ron
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. M. Yoffey
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported in part by a grant from the Joint Research Fund of the Hebrew University and Hadassah, and by a grant from the Deutsche Forschungsgemeinschaft (Pr 75/7)

On leave from the Department of Pediatrics, University of Göttingen, West-Germany, under the auspices of the Committee for Scientific Co-operation between German Institutions and the Weizmann Institute

Rights and permissions

Reprints and permissions

About this article

Cite this article

Prindull, G., Ron, A. & Yoffey, J.M. Cytoplasmic DNA synthesis by cord blood cells of premature and full-term infants. Klin Wochenschr 54, 637–641 (1976). https://doi.org/10.1007/BF01469030

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01469030

Schlüsselwörter

Key words

Search

Navigation