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Plasma Dopamin-β-hydroxylase-activity in dialysed patients

Dopamin-β-Hydroxylaseaktivität im Plasma von Dialysepatienten

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Zusammenfassung

Die Plasma Dopamin-β-Hydroxylase-Aktivität (DBH) wurde bei 70 gesunden Kontrollpersonen und 37 Hämodialysepatienten untersucht. Bei Kontrollpersonen wurde eine basale DBH-Aktivität von 50,0±29,3 IE gefunden. Es bestand kein signifikanter Unterschied zwischen Männern (53,9±33,8 IE) und Frauen (47,4±25 IE). Es wurde keine Korrelation zwischen Lebensalter und basaler DBH-Aktivität gefunden.

Die basale DBH-Aktivität war bei Hämodialysepatienten signifikant (p<0,01) vermindert (32,5±17,6 IE); der Befund ist vereinbar mit verminderter Sympathikusaktivität und/oder Störungen der Freisetzung, des Verteilungsvolumens oder des Abbaues von DBH. Unter Hämodialyse stieg die Plasma DBH-Aktivität während Ultrafiltration an. Dieser Befund belegt eine Aktivierung sympathischer Reflexe durch Volumendepletion bei hämodialysierten Patienten.

Summary

Plasma dopamin-β-hydroxylase (DBH) was studied in 70 healthy control persons and in 37 hemodialysed patients. Basal DBH in controls corresponded to 50.0±29.3 IU. There was no significant difference between males (53.9±33.8 IU) and females (47.4±25 IU); no correlation could be found between age and plasma DBH. In hemodialysed patients basal DBH levels were significantly (p<0.01) decreased (32.5±17.6 IU), suggesting lowered sympathetic activity and/or abnormalities in release, distribution space, or metabolism of DBH. During hemodialysis plasma DBH activity rose during ultrafiltration. This finding indicates a directionally appropriate sympathetic reflex response to volume depletion in dialysed patients.

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References

  1. Brecht, H.M., Ernst, W., Kreh, K.M.: Plasma Noradrenaline Levels in Regular Haemodialysis Patients. Proc. Europ. Dial. Transpl. Ass.12, 281–291 (1975)

    Google Scholar 

  2. Ebstein, R.P., Park, D.H., Freedman, L.S., Levitz, S.M., Ohuchi, T., Goldstein, M.: A Radioimmunoassay of Human Circulatory Dopamine-β-Hydroxylase. Life Sci.13, 679–774 (1973)

    Google Scholar 

  3. Goldberger, S., Thompson, A., Guka, A., Kramer, N., Parrish, A.: Autonomic nervous dysfunction in chronic renal failure. Clin. Res.19, 531 (1971)

    Google Scholar 

  4. Hennemann, H., Hevendehl, G., Herler, E., Heidland, A.: Toxic Sympathicopathy in Uremia. Proc. Europ. Dial. and Transpl. Ass. 10, Pitman Medical, London, 166–170 (1973)

    Google Scholar 

  5. Hennessy, W.J., Siemsen, A.W.: Dysfunction of sudori motoric Glands in Patients with Chronic Renal Failure. Clin. Res.16, 385–391 (1968)

    Google Scholar 

  6. Iversen, L.L.: Catecholamine Uptake Processes. Brit. Med. Bull.29, 130–135 (1973)

    PubMed  Google Scholar 

  7. Kersh, E.S., Kronfield, S.J., Unger, A.: Autonomic Insufficiency in Uremia as a Cause of Hemodialysis-induced Hypotension. N. Engl. J. Med.290, 650–653 (1974)

    PubMed  Google Scholar 

  8. Laduron, P.: Scope and Limitation in Dopamine-beta-Hydroxylase Measurement. Biochem. Pharmacol.24, 557–561 (1975)

    PubMed  Google Scholar 

  9. Lilley, J.J., Goldon, J., Stone, R.A.: Adrenergic — Regulation of Blood Pressure in Chronic Renal Failure. J. clin. Invest.6, 1190–1200 (1976)

    Google Scholar 

  10. Lovenberg, W., Bruckwick, E.A., Alexander, D., Horowitz, D., Keser, H.: Neuropharmacology of Monoamines and Their Regulatory Enzymes. (Ed. E. Usdin.) pp. 121–128, Raven press, N.Y. (1974)

    Google Scholar 

  11. Nagatsu, T., Udenfriend, S.: Photometric Assay of Dopamine-β-Hydroxylase Activity in Human Blood. Clin. Chem.18, 980–983 (1972)

    PubMed  Google Scholar 

  12. Planz, G., Gierlichs, H.W., Hawlina, A., Planz, R., Stephany, W., Rahn, K.H.: A Comparison of Catecholamine Concentrations and Dopamine-β-Hydroxylase Activities in Plasma from Normotensive Subjects and from Patients with Essential Hypertension at Rest and During Exercise. Klin. Wschr.54, 561–565 (1976)

    PubMed  Google Scholar 

  13. Potter, L.T., Axelrod, J.: Properties of Norepinephrine Storage Particles of the Rat Heart. J. Pharmac. exp. Ther.142, 299–305 (1963)

    Google Scholar 

  14. Röckel, A., Hennemann, H., Richwien, D., Heidland, A.: Autonome Insuffizienz bei Dialysepatienten. Verh. dtsch. Ges. inn. Med. 81. Kongreß, pp. 1001–1005 (1975)

  15. Rush, R.A., Geffen, L.B.: Radioimmunoassay and Clearance of Circulating dopamine-Hydroxylase. Circulation Res.31, 444 (1972)

    PubMed  Google Scholar 

  16. Rush, R.A., Thomas, P.E., Nagatsu, T., Udenfriend, S.: Comparison of Human Serum Dopamine-beta-Hydroxylase Levels by Radioimmunoassay and Enzymatic Assay. Proc. natl. Acad. Sci. U.S.A.71, 872–874 (1974)

    PubMed  Google Scholar 

  17. Rush, R.A., Thomas, P.E., Udenfriend, S.: Measurement of Human Dopamine-beta-Hydroxylase in Serum by Homologous Radioimmunoassay. Proc. natl. Acad. Sci., U.S.A.72, 750–752 (1975)

    Google Scholar 

  18. Sharman, D.F.: Catabolism of Catecholamines. Brit. Med. Bull.29, 110–115 (1973)

    PubMed  Google Scholar 

  19. Stone, R.A., Kirshner, N., Gunnells, J.C., Robinson, R.R.: Change of Plasma Dopamine-beta-Hydroxylase Activity and other Plasma Constituents During the Cold Pressor Test. Life Sci.14, 1797–1805 (1974)

    PubMed  Google Scholar 

  20. Tipton, K.F.: Biochemical Aspects of Monoaminooxidase. Brit. Med. Bull.29, 116–119 (1973)

    Google Scholar 

  21. Viveros, L., Arqueros, Kirschner, N.: Release of Catecholamines and Dopamin-β-oxidase from the Adrenal Medulla. Life Sci.7, 609–618 (1968)

    Google Scholar 

  22. Winckler, J., Hennemann, H., Heidland, A., Wigand, M.E.: Katecholamingehalt adrenerger Nerven in Speicheldrüsen mit gestörter Elektrolytausscheidung bei Urämie. Klin. Wschr.51, 1115–1123 (1973)

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Abteilung für klinische Pharmakologie, Sektion für Nephrologie, Innere Medizin III, Medizinische Universitätsklinik Heidelberg, Germany

    U. Spohr, E. Ritz & F. Kaden

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  1. U. Spohr
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  2. E. Ritz
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  3. F. Kaden
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Dedicated to Professor Dr. Dr. G. Schettler on the occasion of his 60th birthday

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Spohr, U., Ritz, E. & Kaden, F. Plasma Dopamin-β-hydroxylase-activity in dialysed patients. Klin Wochenschr 55, 1089–1093 (1977). https://doi.org/10.1007/BF01477935

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