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Histochemical methods for the demonstration of thomsen-friedenreich antigen in cell suspensions and tissue sections

Histochemische Methoden zum Nachweis des Thomsen-Friedenreich-Antigens in Zellsuspensionen und Gewebeschnitten

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Zusammenfassung

1. Drei verschiedene Methoden zur Darstellung von sog. Thomsen-Friedenreich (TF)-Antigenen in Zellsuspensionen, an Formalin-fixiertem und in Paraffin eingebettetem Gewebe sowie in Gefrierschnitten werden beschrieben:

  1. a)

    Rosetten-Bildung mit Hühner- und Schaferythrocyten.

  2. b)

    Fluorescenzmikroskopie mit Fluorescein-markiertem Erdnußagglutinin.

  3. c)

    Autoradiographie mit3H-markiertem Erdnußagglutinin.

2. Nach Neuraminidasebehandlung konnten TF-Antigene — soweit bisher untersucht — auf verschiedenen Blutzellen, in Nierenglomerula und in normalem Brustdrüsengewebe nachgewiesen werden. Ein Teil derartiger Antigene lag im Brustdrüsenparenchym auch bereits als freie Rezeptoren vor, d. h. waren nicht von Neuraminsäure bedeckt.

3. Die Freilegung dieser Kryptantigene kann im Rahmen von Infektionen durch bakterielle und virale Neuraminidase erfolgen. Dabei kommt es zu einer Antigen/Antikörper Reaktion mit unter Umständen klinisch relevanten Folgen.

Summary

1. Three different methods are described for the visualisation of the Thomsen-Friedenreich (TF) antigen on cell suspensions, formalin-fixed and paraffin embedded or frozen tissue sections:

  1. a)

    Rosette formation with chicken and sheep erythrocytes.

  2. b)

    Fluorescence-microscopy with fluorescein labelled peanut agglutinin.

  3. c)

    Autoradiography with3H-labelled peanut agglutinin.

2. The TF antigen was shown, as far as presently investigated, to be exposed on various blood cells, glomerula of the kidney and normal mammary gland after neuraminidase treatment. Mammary gland was also shown to possess TF receptors without prior treatment with neuraminidase.

3. The exposure of this cryptantigen can be brought about by bacterial or viral neuraminidase and is followed by an antigen/antibody reaction, which can lead to possible pathological consequences.

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References

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Authors and Affiliations

  1. Immunbiologische Abteilung der Medizinischen Klinik der Universität Köln, Germany

    G. Uhlenbruck

  2. Pathologisches Institut der Universität, Joseph-Stelzmann-Str. 9, D-5000, Köln 41, Germany

    P. J. Klein, R. A. Newman, P. Müller, G. Uhlenbruck, H. E. Schaefer, K. J. Lennartz & R. Fischer

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  1. P. J. Klein
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  2. R. A. Newman
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  3. P. Müller
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  4. G. Uhlenbruck
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  5. H. E. Schaefer
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  6. K. J. Lennartz
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  7. R. Fischer
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Additional information

Herrn Prof. Dr. R. Gross zum 60. Geburtstag gewidmet

Mit Unterstützung der Deutschen Forschungsgemeinschaft und Alexander von Humboldt Stiftung

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Klein, P.J., Newman, R.A., Müller, P. et al. Histochemical methods for the demonstration of thomsen-friedenreich antigen in cell suspensions and tissue sections. Klin Wochenschr 56, 761–765 (1978). https://doi.org/10.1007/BF01476765

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  • DOI: https://doi.org/10.1007/BF01476765

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