Zusammenfassung
Die Effekte der einzelnen Lipoproteidfraktionen des Plasmas auf die Aktivität der Lipoproteidlipase des menschlichen Fettgewebes wurden untersucht. HDL (high density lipoproteins) und, in geringerem Ausmaß, VLDL (very low density lipoproteins) bewirkten eine dosisabhängige Stimulierung der Aktivität der Lipoproteidlipase. LDL (low density lipoproteins) übten praktisch keinen Effekt aus. In höheren Konzentrationen hemmten sowohl HDL als auch VLDL die stimulierte lipolytische Aktivität. Bei Patienten mit Hyperlipoproteinämie Typ V war der stimulierende Effekt der VLDL und HDL viel geringer, die Hemmwirkung der HDL jedoch deutlich stärker ausgeprägt.
Summary
The effects of the main lipoprotein density classes on the human adipose tissue lipoprotein lipase activity were studied. A dose-dependent stimulation of lipoprotein lipase activity was obtained for HDL and, to a lesser extent, for VLDL on a constant weight basis. LDL exerted virtually no effect. At higher concentrations, HDL as well as VLDL inhibited the stimulated lipolytic activity. In type V hyperlipoproteinemia, the stimulating effect of VLDL and of HDL was significantly lower, whereas the inhibiting action of HDL was markedly increased.
References
Bartlett, G.R.: Phosphorus assay in column chromatography. J. Biol. Chem.234, 466 (1959)
Bensadoun, A., Ehnholm, C., Steinberg, D., Brown, W.V.: Purification and characterization of lipoprotein lipase from pig adipose tissue. J. Biol. Chem.249, 2220 (1974)
Bier, D.M., Havel, R.J.: Activation of lipoprotein lipase by lipoprotein fractions of human serum. J. Lip. Res.11, 565 (1970)
Corey, J.E., Zilversmit, D.B.: Validation of a stable emulsion for the assay of lipoprotein lipase activity. J. Lab. Clin. Med.89, 666 (1977)
Ekman, R., Nilsson-Ehle, P.: Effects of apolipoproteins on lipoprotein lipase activity of human adipose tissue. Clin. Chim. Acta63, 29 (1975)
Fredrickson, D.S., Levy, R.I., Lees, R.S.: Fat transport in lipoproteins–An integrated approach to mechanisms and disorders. New Engl. J. Med.276, 32, 94, 148, 215, 273 (1967)
Havel, R.J., Eder, H.A., Bragdon, J.H.: Distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. clin. Invest34, 1345 (1955)
Havel, R.J., Shore, V.G., Shore, B., Bier, D.M.: Role of specific glycopeptides of human serum lipoproteins in the activation of lipoprotein lipase. Circ. Res.27, 595 (1970)
Havel, R.J., Kane, J.P., Kashyap, M.L.: Interchange of apolipoproteins between chylomicrons and high density lipoproteins during alimentary lipemia in man. J. clin. Invest.52, 32 (1973)
Huang, T.C., Chen, C.P., Wefler, V., Ragtery, A.: A stable reagent for the Liebermann-Burchard reaction. Application to rapid serum cholesterol determination. Anal. Chem.33, 1405 (1961)
Kashyap, M.L., Srivastava, L.S., Chen, C.Y., Perisutti, G., Campbell, M., Lutmer, R.F., Glueck, C.J.: Radioimmunoassay of human apolipoprotein CII. A study in normal and hypertriglyceridemic subjects. J. clin. Invest.60, 171 (1977)
Kessler, G., Lederer, H.: Fluorimetric measurement of triglycerides. In: Automation in analytical chemistry, ed.: L.T. Skeggs, p. 341. New York: Mediac. Inc. 1965
Korn, E.D.: Clearing factor, a heparin-activated lipoprotein lipase. II. Substrate specifity and activation of coconut oil. J. Biol. Chem.215, 15 (1955)
Korn, E.D., Quigley, T.W.: Lipoprotein lipase of chicken adipose tissue. J. Biol. Chem.226, 833 (1957)
LaRosa, J.C., Levy, R.I., Herbert, P., Lux, S.E., Fredrickson, D.S.: A specific apoprotein activator for lipoprotein lipase. Biochem. Biophys. Res. Comm.41, 57 (1970)
Noble, R.P.: Electrophoretic separation of plasma lipoproteins in agarose gel. J. Lip. Res.9, 693 (1968)
Patsch, J.R., Sailer, S., Braunsteiner, H.: Lipoprotein of the density 1.006–1.020 in the plasma of patients with type III hyperlipoproteinemia in the postabsorptive state. Europ. J. clin. Invest.5, 45 (1975)
Scanu, A.M., Edelstein, C., Keim, P.: Serum lipoproteins. In: The plasma proteins, 2nd ed., Vol. 1, ed.: F.W. Putnam, p. 326. New York: Academic Press 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lisch, H.J., Patsch, W., Riedler, L. et al. Activation of adipose tissue lipoprotein lipase by lipoprotein fractions from normals and patients with type V hyperlipoproteinemia. Klin Wochenschr 56, 1067–1069 (1978). https://doi.org/10.1007/BF01476554
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01476554