Zusammenfassung
10 min-Proben von Magensaft wurden mit Hilfe der nasogastrischen Aspiration während maximaler Histaminstimulation bei 14 Personen gesammelt (6 Normalpersonen, 8 Patienten mit Ulcus duodeni). Bei 8 dieser Untersuchungen wurde eine gleichzeitige Infusion mit Indocyanin Grün (ICG) verwendet, um die Galle zu markieren. Eine intravenöse Einzelinjektion von Boots-Sekretin wurde während der maximalen Plateausekretion gegeben und weitere Proben wurden gesammelt.
Volumen, titrierbare Azidität, Elektrolyt- und I.C.G.-Konzentrationen wurden gemessen.
Veränderungen im Volumen und in den Elektrolytkonzentrationen nach Sekretin stimmten mit der Hypothese überein, daß Sekretin den duodenogastrischen Reflux stimuliert und daß der duodeno-gastrische Reflux die Hauptdeterminante für spontane Variationen in den Elektrolytkonzentrationen im Magenaspirat ist.
Der duodeno-gastrische Reflux wurde mit einer früher beschriebenen Formel berechnet, die auf dem Natriumgehalt des Aspirates basiert. Dies erwies sich als gut korreliert mit der Konzentration des ICG im Aspirat.
Summary
Ten minute samples of gastric juice were collected by naso-gastric aspiration during maximal histamine stimulation in 14 subjects (6 normal, 8 with duodenal ulceration). In eight of these studies, a simultaneous intravenous infusion of Indocyanine green (ICG) was used to label the bile. A single intravenous injection of Boots secretin was given during the maximal plateaux, and further samples collected.
The volume, titratable acidity, electrolyte concentrations and ICG concentrations were measured.
Changes in volume and in electrolyte concentrations after secretin were consistent with the hypothesis that secretin stimulated duodenogastric reflux, and that duodenogastric reflux was the major determinant of spontaneous variations in electrolyte concentrations in gastric aspirate.
Duodenogastric reflux was calculated by a previously described formula based on the sodium content of the aspirate, and this was found to correlate well with the concentration of the ICG label in the aspirate.
This is a preview of subscription content, log in via an institution to check access.
References
Brooks, A.M., Grossman, M.I.: Effect of secretin and cholecystokinin on pentagastrin-stimulated gastric secretion in man. Gastroenterology59, 114–119 (1970)
Cherrick, G.R., Stein, S.W., Leevy, C.M., Davidson, C.S.: Indocyanine green: Observations on its physical properties, plasma decay and hepatic secretion. J. Clin. Invest.39, 592–600 (1960)
Chey, W.Y., Hitnant, S., Hendricks, J., Lorber, S.H.: Effect of secretin and cholecystokinin on gastric emptying and secretion in man. Gastroenterology58, 820–827 (1970)
Crawford, G., Hobsley, M.: Spectrophotometric estimation of phenol red in gastric juice in the presence of blood. Biochem. J.107, 26 (1968)
Du Plessis, D.J.: Pathogenesis of gastric ulceration. Lancet1965 I, 974–976
Faber, R.G., Russell, R.C.G., Parkin, J.V., Whitfield, P., Hobsley, M.: The predictive accuracy of the post-vagotomy insulin test: a new interpretation. Gut16, 337–342 (1975)
Faber, R.G., Russell, R.C.G., Royston, C.M.S., Whitfield, P., Hobsley, M.: Duodenal reflux during insulin-stimulated secretion. Gut15, 880–884 (1974)
Hassan, M.A., Hobsley, M.: Positioning of subject and nasogastric tube during a gastric secretion study. Br. Med. J.1, 458–460 (1970)
Hirschowitz, B.I.: Electrolytes in human gastric secretion. Am. J. Dig. Dis.6, 199–228 (1961)
Hobsley, M.: Pyloric reflux: A modification of the two-component hypothesis of gastric secretion. Clin. Sci. Mol. Med.47, 131–141 (1974)
Hobsley, M., Silen, W.: Use of an inert marker (phenol red) to improve accuracy in gastric secretion studies. Gut10, 787–795 (1969)
Hobsley, W., Silen, W.: The relation between the rate of production of gastric juice and its electrolyte concentration. Clin. Sci.39, 61–75 (1970)
Hollander, F.: Studies in gastric secretion IV Variations in the chlorine content of gastric juice and their significance. J. biol. Chem.97, 585–604 (1932)
Johnson, L.R., Grossman, M.I.: Characteristics of inhibition of gastric secretion by secretin. Am. J. Physiol.217, 1401–1414 (1969)
Konturek, S.J.: Comparison of pancreatic responses to natural synthetic secretins in conscious cats. Am. J. Dig. Dis.14, 557–565 (1969)
Lawrie, J.H., Smith, G.M.R., Forrest, A.P.M.: The histamine infusion test. Lancet2, 270–273 (1964)
Makhlouf, G.M., McManus, J.P.A., Card, W.I.: A qualitative statement of the two-component hypothesis of gastric secretion. Gastroenterology51, 149–171 (1966)
Peterson, H., Berstad, A.: Comparison of response to intravenous injection and infusion of secretin in man. Scand. J. Gastroenterol.7, 463–469 (1972)
Teorell, T.: Electrolyte diffusion in relation to the acidity regulation of the gastric juice. Gastroenterology,9, 425–443 (1947)
Thomson, J.P.S., Russell, R.C.G., Hobsley, M.: Double-lumen tube for gastric secretion studies. Br. Med. J.1, 175 (1973)
Ward, A.S., Bloom, S.R.: The role of secretin in the inhibition of gastric secretion by intraduodenal acid. Gut15, 889–897 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fiddian-Green, R.G., Parkin, J.V., Faber, R.G. et al. The quantification in human gastric juice of duodenogastric reflux by sodium output and by bile-labelling using indocyanine green. Klin Wochenschr 57, 815–824 (1979). https://doi.org/10.1007/BF01477986
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01477986