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Solid-phase radioimmunoassay for hepatitis be antigen (HBeAg)

Solid-Phase Radioimmunoassay für Hepatitis Be Antigen (HBeAg)

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Zusammenfassung

Ein Radioimmunoassay wurde entwickelt zum Nachweis von HBeAg und anti-HBe in Serum oder Serumfraktionen. HBe/sAg positives Serum, teilweise gereinigtes HBeAg, teilweise gereinigtes HBsAg und HBe/sAg negatives Kontrollserum wurden in Polyacrylamid polymerisiert und auf ihre Bindungsfähigkeit für125I-IgG (anti-HBe) untersucht. Nur Gele mit immobilisiertem HBeAg reagierten spezifisch mit anti-HBe. Die Spezifität der Bindung wurde gesichert durch Blockierungs-/Inhibitionsteste mit anti-HBe, HBeAg, HBsAg und negativen Kontrollseren. Der Radioimmunoassay ermöglicht den spezifischen und quantitativen Nachweis von HBeAg und anti-HBe, auch in Gegenwart von Detergentien und hohen Salzkonzentrationen.

Summary

A solid-phase radioimmunoassay was developed for the detection of HBeAg and anti-HBe in sera or serum fractions. HBe/sAg positive sera, partially purified HBeAg, partially purified HBsAg, and HBe/sAg negative sera were polymerized in polyacrylamide and compared for their ability to bind125I-IgG (anti-HBe). Only gels containing HBeAg reacted specifically with the iodinated antibody. The specificity of the binding was confirmed by blocking and inhibition tests using anti-HBe, HBeAg, HBsAg, and negative control sera. The radioimmunoassay allows the specific and quantitative detection of HBeAg and anti-HBe even in the presence of detergents and high salt concentrations.

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Abbreviations

HBsAg:

hepatitis B surface antigen

HBeAg:

hepatitis Be antigen

HBe/sAg:

hepatitis Be antigen and surface antigen

anti-HBe:

antibody to hepatitis Be antigen

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Authors and Affiliations

  1. Medizinische Klinik der Universität Freiburg i. Br., Germany

    H. E. Blum, G. Dölken &  W. Gerok

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  1. H. E. Blum
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  2. G. Dölken
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  3. W. Gerok
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Blum, H.E., Dölken, G. & Gerok, W. Solid-phase radioimmunoassay for hepatitis be antigen (HBeAg). Klin Wochenschr 57, 1129–1132 (1979). https://doi.org/10.1007/BF01481494

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  • DOI: https://doi.org/10.1007/BF01481494

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