Skip to main content
Log in

Thrombopoese, Thrombozytenzahl und Thrombozytenfunktion vor und nach Zellseparation

Thrombopoesis, platelet count and platelet function before and after cell separation

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Summary

About 50% of the circulating platelets can be taken from healthy donors by means of the cell separation technique. In order to investigate the effect of cell separation on thrombopoesis bone marrow samples were taken from 12 voluntary donors up to 5 times. The megacaryocytes were analyzed before and after cell separation by morphometric measures and determinations of platelet count and platelet function (adhesion, aggregation, spreading, retraction) were carried out. Removing of large amounts of platelets form the circulation by stimulation of thrombopoesis resulted in an increase of the peripherical platelet count already 48 h after cell separation. Of the platelet functions tested only aggregation induced by collagen was found to be increased after cell separation as compared to that before the procedure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Craddock CG, Adams WS, Perry S, Lawrence JS (1955) Dynamics of platelet production as studied by depletion technique in normal and irradiated dogs. J Lab Clin Med 45:906

    PubMed  Google Scholar 

  2. Ebbe S, Stohlmann F, Overcash J, Donovan J, Howard D (1968) Megakaryocyte size in thrombocytopenic and normal rats. Blood 32:383

    PubMed  Google Scholar 

  3. Harker LA (1968) Kinetics of thrombopoiesis. J Clin Invest 47:458

    PubMed  Google Scholar 

  4. Linker H, Reuter H, Gross R (1975) Diagnostik und klinische Bedeutung von Thrombozytenfunktionsstörungen. Ärztl Lab 8:291

    Google Scholar 

  5. Matter M, Hartmann JR, Kautz J, Demarsh OB, Finsh CA (1960) A study of thrombopoiesis in induced acute thrombocytopenia. Blood 15:174

    Google Scholar 

  6. Penington DG, Olson TE (1970) Megakaryocytes in states of altered plateletproduction: Cell numbers, size and DNA content. Brit J Haematol 18:447

    Google Scholar 

  7. Wichmann HE, Gerhardts MD, Spechtmeyer H, Gross R (1979) A mathematical model of thrombopoiesis in rats. Cell Tissue Kinet 12:551

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Herrn Prof. Dr. R. Gross zum 1. 10. 1983 gewidmet

Mit Unterstützung des Ministers für Wissenschaft und Forschung des Landes Nordrhein-Westfalen

Rights and permissions

Reprints and permissions

About this article

Cite this article

Linker, H., Reuter, H.D., Eckers, G. et al. Thrombopoese, Thrombozytenzahl und Thrombozytenfunktion vor und nach Zellseparation. Klin Wochenschr 61, 969–973 (1983). https://doi.org/10.1007/BF01550270

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01550270

Key words

Navigation