Summary
Fructose-lysine, which is formed by binding glucose to lysine, is degraded on acid hydrolysis into furosine. Furosine was determined by high-performance liquid chromatography according to the method of Schleicher et al. Furosine values were significantly higher in diabetic patients than in healthy subjects, and significantly correlated with haemoglobin A1 (HbA1) values. These results suggest that furosine, like HbA1, may become an indicator of long-term blood glucose control in diabetic patients and be useful in investigating diabetic complications on the level of tissue.
References
Brüggemenn J, Erbersdobler H (1968) Fructoselysin als wichtigstes Reaktionsprodukt von Lysin mit Glucose bei Hitzebehandlung von Lebens- und Futtermitteln. Z Lebensm Unters Forsch 137:137–143
Bunn HF, Haney DN, Gabbay KH, Gallop PM (1975) Further identification of the nature and linkage of the carbohydrate in hemoglobin A1c. Biochem Biophys Res Commun 67:103–109
Carpenter KJ, Booth VH (1973) Damage to lysine in food processing; its measurement and significance. Nutr Abstr. Rev 43:423–451
Cohen MP, Urdanivia E, Surma M, Wu V (1980) Increased glycosylation of glomerular basement membrane collagen in diabetics. Biochem Biophys Res Commun 95:765–769
Dolhofer R, Wieland OH (1979) Preparation and biological properties of glycosylated insulin. FEBS Lett 100:133–136
Flückiger R, Winterhalter KH (1976) In vitro synthesis of hemoglobin A1c. FEBS Lett 71:356–360
Gabbay KH, Hasty K, Breslow JL, Ellison C, Bunn HF, Gallop PM (1977) Glycosylated hemoglobins and long-term blood glucose control in diabetes mellitus. J Clin Endocrinol Metab 44:859–864
Miller JA, Gravalese E, Bunn HF (1980) Non-enzymatic glycosylation of red cell membrane proteins: relevance to diabetes. J Clin Invest 65:896–901
Oimomi M, Yoshimura Y, Kawasaki T, Kubota S, Tanke G, Baba S (1981) Synthesis of glycosylated triiodothyronine in vitro. Endocrinol Jpn 28:277–279
Oimomi M, Kawasaki T, Kubota S, Yoshimura Y, Baba S (1984) Aspects of colorimetric method using thiobarbituric acid for the determination of glycosylated hemoglobin. Acta Haematol Jpn, in press
Rosenberg H, Madrak JB, Hassing JM, Al-Turk WA, Strohs SJ (1979) Glycosylated collagen. Biochem Biophys Res Commun 91:498–501
Schleicher E, Wieland OH (1981) Specific quantitation by HPLC of protein (lysine) bound glucose in human serum albumin and other glycosylated proteins. J Clin Chem Biochem 19:81–87
Stevens VJ, Ronzer CA, Monnier VM, Cerami A (1978) Diabetic cataract formation: potential role of glycosylation and lens crystallins. Proc Natl Acad Sci 75:2918–2922
Vogt BW, Schleicher ED, Wieland OH (1982) ε-Aminolysine-bound glucose in human tissues obtained at autopsy. Diabetes 31:1123–1127
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Oimomi, M., Hatanaka, H., Ishikawa, K. et al. Increased fructose-lysine of nail protein in diabetic patients. Klin Wochenschr 62, 477–478 (1984). https://doi.org/10.1007/BF01726910
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DOI: https://doi.org/10.1007/BF01726910