Summary
Serum factors may be responsible for reduced host-anti-tumor defence. Although there is still confusion about their origin, attempts have been made to immobilize serum components by Protein A columns as a therapeutic modality. In our study the in vitro adsorption of 90% of the IgG from cancer sera on “immobilized protein A” did not influence the inhibitory serum activity as measured in a mixed lymphocyte culture. Therefore, IgG or immune complexes do not seem to be the suppressive serum factor in patients with advanced colorectal carcinoma. There is evidence for leakage of small amounts of protein A from the columns which have immunostimulatory activity. Perhaps this may explain necrosis after a therapeutic immunoadsorption.
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Abbreviations
- CEA:
-
Carcinoembryonal antigen
- C Effluent:
-
Unadsorbed fraction
- C Eluate:
-
Adsorbed fraction
- CP:
-
Cancer plasma
- CPG:
-
Controlled pore glass beads
- CPM:
-
Counts per minute
- C Sera:
-
Sera from patients with metastatic colorectal cancer
- HSA:
-
Human serum albumin
- 3H-Tdr:
-
3H-thymidine
- MLC:
-
Mixed lymphocyte culture
- NP:
-
Control plasma
- N Sera:
-
Sera from healthy persons
- PBMNC:
-
Peripheral blood mononuclear cells
- PBS:
-
Phosphate-buffered saline
- PI:
-
Untreated plasma probes
- RPMI 1640:
-
Culture medium (Ross Park Memorial Institute)
- SAC:
-
Staphylococcus aureus Cowan I
- SAW:
-
Staphylococcus aureus Wood
- SpA:
-
Purified staphylococcal protein A
References
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Supported by the BMFT, project no. 03/8410/0
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Richner, J., Schuff-Werner, P., Bätge, R. et al. In vitro adsorption of colon cancer sera over staphylococcus protein a: lymphocyte stimulation by leakage of adsorbance. Klin Wochenschr 65, 353–358 (1987). https://doi.org/10.1007/BF01745571
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DOI: https://doi.org/10.1007/BF01745571