Skip to main content
SpringerLink
Log in

Das erregerspezifische Antikörperprofil in den verschiedenen Stadien der HIV-1-Infektion

The virusspecific pattern of antibody response in different stages of HIV-1-infection — Westernblot analysis of 170 patients

Western blot analyse von 170 patienten

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Summary

The westernblot analysis of 170 patients with HIV-1-infection demonstrated that 47% of the patients in latent stage, 58% of the patients with lymphadenopathy-syndrome and only 25% of the patients with the full-blown picture of AIDS showed the complete pattern of HIV-specific antibody response. This antibody response is mainly directed against the env-encoded envelope proteins gp160, gp120 and gp41, against the gag-encoded core proteins p55, p24 and p17 as well as against the pol-encoded enzymatic proteins p66, p51 and p31.

Antibodies against gp160 and gp120 were present in nearly all patients, whereas the prevalence of the other antibodies decreased with the stage of the disease. Statistical significant differences were found particularly between patients with LAS or AIDS respectively. Antibodies against p17 were detected in 74% of the patients with LAS but only in 25% of the patients with AIDS. The lack of antibodies against p17, p24 or p51 was significantly associated with lower mean CD4/CD8-ratios (p<0,007) and higher mean serum levels of IgA (p<0,001) and beta-2-microglobulin (p<0.001). One third of the patients with LAS and this reduced pattern of antibody response developed AIDS within six months. These results demonstrate that the detection of antibodies against p17, p24 or p51 is of prognostic importance. A serological profile which lacks the antibody response against at least two of those three viral antigens indicates a progression of the disease activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

LAS:

Lymphadenopathie-Syndrom

ELISA:

Enzyme-linked-Immunosorbent-Assay

Literatur

  1. Bhalla RB, Safai B, Pahwa S, Schwartz MK (1985)β 2-Microglobulin as a prognostic marker for development of AIDS. Clin Chemistry 31:1411–1412

    Google Scholar 

  2. Centers for Disease Control (1986) Classification system for human T-lymphotropic virus type III/lymphadenopathy-associated virus infections. Morbid Mortal wkly Rep 30:305–308

    Google Scholar 

  3. Franchini G, Robert-Guroff M, Aldovini A, Kan NC, Wong-Staal F (1987) Spectrum of natural antibodies against five HTLV-III antigens in infected individuals: correlation of antibody prevalence with clinical status. Blood 69:437–441

    Google Scholar 

  4. Francioli P, Clement F, Glausner MP (1984)β 2-Microglobulin: a sensitive non-specific marker of acquired immunodeficiency syndrome. Eur J Clin Microbiol 3:68–69

    Google Scholar 

  5. Frösner GG, Erfle V, Mellert W, Hehlmann R (1987) Diagnostic significance of quantitative determination of HIV antibody specific for envelope and core proteins. Lancet I:159–160

    Google Scholar 

  6. Gaines H, Sönneborg A, Czajkowski J, Chiodi F, Fenyö EM, von Sydow M, Albert J, Pehrson J, Moberg L, Asjö B, Forsgren M (1987) Antibody response in primary human immunodeficiency virus infection. Lancet I:1249–1253

    Google Scholar 

  7. Goudsmit J, Lange JMA, Paul DA, Dawson GJ (1987) Antigenemia and antibody titers to core and envelope antigens in AIDS, AIDS-related complex, and subclinical human immunodeficiency virus infection. J Infect Diseases 155:558–560

    Google Scholar 

  8. Groopman JE, Chen FW, Hope JA, Andrews JM, Swift RL, Benton CV, Sullivan JL, Volberding PA, Sites DP, Landesman S, Gold J, Baker L, Craven D, Boches FS (1986) Serological characterization of HTLV-III Infection in AIDS and related disorders. J Infect Diseases 153:736–742

    Google Scholar 

  9. Gürtler LG, Eberle J, Lorbeer B, Deinhardt F (1987) Sensitivity and specifity of commercial ELISA kits for screening anti-LAV/HTLV-III. J Virol Meth 15:11–23

    Google Scholar 

  10. Hehlmann R, Fischer AM, Matuschke A, Mellert W, Frösner G, Goebel FD, Erfle V (1987) Development of HIV Markers during the later stages of HIV infection. AIFO 8:441–447

    Google Scholar 

  11. Joller-Jemelka J, Vogt J, Joller PW (1985) Immunologische Laboruntersuchungen bei Patienten mit erworbenem Immunmangelsyndrom (AIDS) und bei AIDS-Verdacht. Schweiz med Wochenschr 115:125–132

    Google Scholar 

  12. Lange JMA, Coutinho RA, Krone WJA, Verdonick LF, Danner SA, van der Noorda J, Goudsmit J (1986) Distinct IgG recognition patterns during progression of subclinical and clinical infection with lymphadenopathy associated virus/human T lymphotropic virus. Br Med J 292:228–292

    Google Scholar 

  13. Lange JMA, Paul DA, Huisman HG et al. (1986) Persistant HIV antigenaemia and decline of HIV core antibodies associated with transition to AIDS. Br Med J 154:171–174

    Google Scholar 

  14. Mellert W, Kühnl P, Frösner G, Erfle V, Hehlmann R (1987) Comparison of different serological tests for the detection of antibodies against HTLVIII/LAV (HIV): Validity of the indirect immunoperoxidase staining. AIFO 3:145–149

    Google Scholar 

  15. Meurer M, Braun-Falco O (1986) Serodiagnostik der LAV/HTLV-III-Infektion in der Praxis. Münch Med Wochenschr 128:276–280

    Google Scholar 

  16. Pan L, Cheng-Mayer C, Levy JA (1987) Patterns of antibody response in individuals infected with the human immunodeficiency virus. J Infect Diseases 155:626–631

    Google Scholar 

  17. Ranki A, Krohn M, Allain JP, Franchini G, Valle SL, Antonen J, Leuther M, Krohn K (1987) Long latency precedes overt seroconversion in sexually transmitted human-immunodeficiency-virus infection. Lancet II:589–593

    Google Scholar 

  18. Ranki A, Weiss SH, Valle S-L, Antonen J, Krohn KJE (1987) Neutralizing antibodies in HIV (HTLV-III) infection: correlation with clinical outcome and antibody response against different viral proteins. Clin exp Immunol 69:231–239

    Google Scholar 

  19. Sarngadharan MG, Popovic M, Bruch R, Schüpbach J, Gallo RC (1984) Antibodies reactive with human T-lymphotropic retrovirus (HTLVIII) in the serum of patients with AIDS. Science 224:506–508

    Google Scholar 

  20. Saxinger C, Gallo RC (1983) Application of the Indirect Enzyme-Linked Immunosorbent Assay Microtest to the Detection and Surveillance of Human T Cell Leukemia-Lymphoma Virus. Lab Invest 49:371–377

    Google Scholar 

  21. Schüpbach J, Haller O, Vogt M, Lüthy R, Joller H, Oelz O, Popovic M, Sarngadharan MC, Gallo C (1985) Antibodies to HTLV-III in swiss patients with AIDS und pre-AIDS and in groups at risk for AIDS. N Engl J Med 312:265–270

    Google Scholar 

  22. Schulte C, Meurer M, Braun-Falco O, Fröschl M, Ring J, Riethmüller G, Gürtler L (1987) Serologische und immunologische Diagnostik der HIV-1-Infektion. Dtsch Med Wochenschr 112:1687–1693

    Google Scholar 

  23. Schulte C, Meurer M, Fröschl M (1988) Prognostische Bedeutung von Antikörpern gegen HIV-Kernproteine bei Patienten mit Lymphadenopathie-Syndrome. Hautarzt 39:45–48

    Google Scholar 

  24. Vadhan-Raj S, Wong G, Gnecco C, Cunningham-Rundles S, Krim M, Real FX, Oettgen HF, Krown SE (1986) Immunological Variables as Predictors of prognosis in patients with Kaposi's sarcoma and the acquired immunodeficiency syndrome. Cancer Research 46:417–425

    Google Scholar 

  25. Weber JN, Weiss RA, Roberts C, Weller I, Tedder RS et al. (1987) Human immunodeficiency virus infection in two cohorts of homosexual men: neutralising sera and association of anti-gag antibody with prognosis. Lancet I:19–122

    Google Scholar 

  26. Tsang VCW, Peralta JM, Simons AR (1983) Enzyme-linked Immunoelectrotransfer Blot Techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods in Enzymology 92:377–391

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dermatologische Klinik und Poliklinik, Ludwig-Maximilians-Universität München, Germany

    C. Schulte, M. Meurer, O. Braun-Falco, M. Held & M. Fröschl

Authors
  1. C. Schulte
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Meurer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Braun-Falco
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Held
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Fröschl
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Herrn Prof. Dr. N. Zöllner zum 65. Geburtstag gewidmet

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schulte, C., Meurer, M., Braun-Falco, O. et al. Das erregerspezifische Antikörperprofil in den verschiedenen Stadien der HIV-1-Infektion. Klin Wochenschr 66, 488–493 (1988). https://doi.org/10.1007/BF01876170

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01876170

Key words

Search

Navigation