Summary
The inhibition of lipid peroxidation of erythrocyte membranes by glutathione-dependent protection was studied in patients with non-insulin dependent diabetes mellitus. Incubation of red cells from diabetics with 1.5 mM t-butyl hydroperoxide resulted in a lipid peroxidation increase greater than that of normal controls. Glutathione-dependent and glutathione-independent protection against oxidative damage was examined using an artificial system, in which erythrocyte ghosts were incubated with t-butyl peroxide and dialysed hemolysate in the presence or the absence of 2 mM glutathione. The glutathione-dependent protection of hemolysate from diabetics was approximately 70% of that from normal controls.
The results suggest that decrease in glutathione-dependent protection against lipid peroxidation, along with decrease in glutathione levels, increases oxidative damage in erythrocyte membranes taken from diabetic patients.
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Abbreviations
- GSH:
-
reduced form of glutathione
- GSSG:
-
glutathione disulfide
- TCA:
-
trichloracetic acid
- SOD:
-
superoxide dismutase
- GPX:
-
glutathione peroxidase
- MDA:
-
malondialdehyde
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Fujiwara, Y., Kondo, T., Murakami, K. et al. Decrease of the inhibition of lipid peroxidation by glutathione-dependent system in erythrocytes of non-insulin dependent diabetics. Klin Wochenschr 67, 336–341 (1989). https://doi.org/10.1007/BF01741388
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DOI: https://doi.org/10.1007/BF01741388