Summary
Highly repeated nuclear DNA sequences from suspension cultured cells of Oryza sativa L. cv. ‘Roncarolo’ have been cloned in pBR322. Ten clones with specific digestion patterns have been randomly selected. Nine sequences appear to be organized in a clustered tandem array while one is interpersed in the rice genome. The clones have been used to gather information on: (a) their modulation in cultured cells as compared to whole plant and (b) their distribution in different rice cultivars belonging to the Japonica or Indica subspecies of Oryza sativa L. Hybridization with nuclear DNA isolated either from suspension or from seedlings of the ‘Roncarolo’ cultivar revealed extensive quantitative variations, with most cloned sequences showing amplification (up to 75-fold) in cultured cells. Hybridization with nuclear DNA isolated from seedlings or suspension cultured cells from different cultivars belonging to the Japonica or to the Indica sub-species of O. sativa have shown that (a) amplification also occurs in a similar pattern in the case of DNA from the other tested suspension cultured cell types but not in the case of DNA from seedlings; (b) in some cases the tested sequences show minor but significant variations in different rice accessions.
Similar content being viewed by others
References
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Blin N, Stafford DW (1976) A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res 3:2303–2308
Cohen SN, Chang ACY, Hsu L (1972) Non chromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114
D'Amato F (1978) Chromosome number variation in cultured cells and regenerated plants. In: Thorpe TA (ed) Frontiers of plant tissue culture. University of Calgary Offset Printing Service, Calgary, pp 287–295
De Paepe R, Prat D, Huguet T (1982) Heritable nuclear DNA changes in double haploid plants obtained by pollen culture of Nicotiana sylvestris. Plant Sci Lett 28:11–28
Evans IJ, James AM, Barnes SR (1983) Organization and evolution of repeated DNA sequences in closely related plant genomes. J Mol Biol 170:803–826
Flavell RB (1982) Sequence amplification, deletion and rearrangement: major sources of speciation during species divergence. In: Dover GA, Flavell RB (eds) Genome evolution. Academic Press, London, pp 301–325
Gasser CS, Simonsen CC, Schilling JW, Schimke RT (1982) Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells. Proc Natl Acad Sci USA 79:6522–6526
Giuliano G, Rosillini D, Terzi M (1983) A new method for the purification of the different stages of carrot embryoids. Plant Cell Rep 2:216–218
Grunstein M, Hogness (1975) Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3965
Kafatos FC, Jones CW, Efstratiadis A (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res 7:1541–1552
Kirk JTO, Rees H, Evans G (1970) Base composition of nuclear DNA within the genus Allium. Heredity 25:507–512
Lai KL, Liu LF (1978) Studies on the rice protoplasts. Ultrastructural changes during enzymatic isolation. J Agri Assoc of China, N S 102:11–23
Maniatis T, Fritsch EF, Sambrood J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor
Metzlaff M, Troebner W, Baldauf F, Schlegel R, Cullum J (1986) Wheat specific repetitive DNA sequences-construction and characterization of four different genomic clones. Theor Appl Genet 72:207–210
Negruk VI, Eisner GI, Redichkina TD, Dumanskaya NN, Cherny DI, Alexadrov AA, Shemyakin MF, Butenko RG (1986) Diversity of Vicia faba circular mtDNA in wholo plants and suspension cultures. Theor Appl Genet 72:541–547
Nielsen E, Rollo F, Parisi B, Cella R, Sala F (1979) Genetic markers in cultured plant cells: differential sensitivity to amethopterin, azetidine-2-carboxylic acid and hydroxyurea. Plant Sci Lett 15:113–125
Oono K, Kikuchi S, Takaiwa (1986) DNA amplification and diminution in rice callus culture. In: Somers DA, Gengenbach BG, Biesboer DD, Backett WP, Green CE (eds) Abstracts of 6th Int Congr Plant Tissue Cell Culture. University of Minnesota, Minneapolis Minn, (USA), p 287
Pental D, Barnes SR (1985) Interrelationship of cultivated rices Oryza sativa and O. glaberrima with wild O. perennis complex. Theor Appl Genet 70:185–191
Ranjekar PK, Pallotta D, Lafontaine JG (1978) Analysis of plant genomes. 5. Comparative study of molecular properties of DNAs of seven Allium species. Biochem Genet 16:957–971
Sala C, Biasini MG, Morandi C, Nielsen E, Parisi B, Sala F (1985) Selection and nuclear DNA analysis of cell hybrids between Daucus carota and Oryza sativa. J Plant Physiol 118:409–419
Sala F, Cella R, Rollo F (1979) Freeze-preservation of rice cells grown in suspension culture. Physiol Plant 45:170–176
Saul MW, Potrykus I (1984) Species-specific repetitive DNA used to identify interspecific somatic hybrids. Plant Cell Reps 3:65–67
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Author information
Authors and Affiliations
Additional information
Communicated by F.Salamini
On leave from China National Rice Research Institute, Hangzhou, China
Rights and permissions
About this article
Cite this article
Zheng, K.L., Castiglione, S., Biasini, M.G. et al. Nuclear DNA amplification in cultured cells of Oryza sativa L.. Theoret. Appl. Genetics 74, 65–70 (1987). https://doi.org/10.1007/BF00290085
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00290085