Summary
Separations of kafirin and alcohol soluble glutelin proteins by reversed-phase high-performance liquid chromatography (RP-HPLC) from 7 inbreds and one hybrid of sorghum [Sorghum bicolor (L.) Moench] and one source of Johnsongrass [Sorghum halapense (L.) Pers.] were compared. Objectives were to assess the stability of protein profiles for seed sources produced at different locations and in different environments to examine the potential of RP-HPLC to provide genotypic profiles for sorghum. Analyses of variance data showed that levels of variation due to environments and locations were small; the majority of variation (93%) was among genotypes. Associations among inbreds revealed by multivariate and cluster analysis showed similarity with those that would be expected on the basis of pedigree. A chi-square analysis showed no deviation in the hybrid profile from the expected 2∶1 ratio of peaks from the female and male inbred parents, respectively. Improvements in the ability to correctly assign common peaks are necessary before associations among numerous sorghum genotypes can be reliably demonstrated by analysis of data from reversed-phase high-performance liquid chromatography (RP-HPLC).
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References
Bietz JA (1982) Cereal prolamin evolution and homology revealed by sequency analysis. Biochem Genet 20:1039–1053
Bietz JA (1983) Separation of cereal proteins by reversed-phase high-performance liquid chromatography. J Chromatogr 155:219–238
Bietz JA (1986) High performance liquid chromatography of cereal proteins. Adv Cereal Sci Technol 8:105–170
Cooke RJ (1984) The characterisation and identification of crop cultivars by electrophoresis. Electrophoresis 5:59–72
Delannay X, Rodgers DM, Palmer RG (1983) Relative genetic contributions among ancestral lines to North American soybean cultivars. Crop Sci 23:944–949
Draper SR, Cooke RJ (1984) Biochemical tests for cultivar identification. Int Seed Testing Assoc. Zürich, Switzerland
Higgins TJV (1984) Synthesis and regulation of major proteins in seeds. Annu Rev Plant Physiol 35:191–221
Kempthorne O (1969) An introduction to genetic statistics. Iowa State University Press, Ames, IA
Marchylo BA, Kruger JE (1984) Identification of Canadian barley cultivars by reversed-phase high-performance liquid chromatography. Cereal Chem 61:295–301
Paulis JW, Wall JS (1979) Distribution and electrophoretic properties of alcohol-soluble proteins in normal and highlysine sorghum. Cereal Chem 56:20–23
Sapirstein HD, Bushuk W (1986) Computer-aided analysis of gliadin electrophoregrams. I. Improvement of precision of relative mobility determination by using a three reference band standardization. Cereal Chem 62:372–377
Sastry LVS, Paulis JW, Bietz JA, Wall JS (1986) Genetic variation of storage proteins in sorghum grain: studies by isoelectric focusing and high-performance liquid chromatography. Cereal Chem 63:420–427
Shechter Y, DeWet JMJ (1975) Comparative electrophoresis and isozyme analysis of seed proteins from cultivated races of sorghum. Am J Bot 62:254–261
Smith JSC (1986) Biochemical fingerprints of cultivars using reversed-phase high-performance liquid chromatography and isozyme electrophoresis: a review. Seed Sci Technol 14:753–768
Smith JSC, Smith OS (1987) Associations among inbred lines of maize using electrophoretic, Chromatographie, and pedigree data. Part 1. Multivariate and cluster analysis of data from Lancaster Sure Crop derived lines. Theor Appl Genet 73:654–664
Soave C, Salamini F (1983) Genetic organization and regulation of maize storage proteins. In: Daussant J, Mosse J, Vaughan J (eds) Seed proteins. Academic Press, London, pp 205–218
Tanksley SD, Orton TJ (1983) Isozymes in plant genetics and breeding. Parts A and B. Elsevier, Amsterdam
Taylor JN, Schussler L (1984) Sorghum cultivar verification by electrophoresis. J Sci Food Agric 35:1–8
Thomas-Compton MA, Gardner CO (1982) Electrophoresis studies in corn and sorghum populations. Mimeo Department of Agronomy, University of Nebraska, Lincoln, NE
Tripathi DP, Dongre AB, Mehta SL, Roa NGP (1982) Soluble protein and esterase isoenzyme patterns on isoelectric focusing from seeds and anthers of diverse cytoplasmic genic male sterile sorghums [Sorghum bicolor (L.) Moench]. Z Pflanzenzücht 88:69–78
Tripathi DP, Mehta SL, Rao NGP (1983) Soluble proteins and isoenzymes from seeds of diverse male steriles of sorghum [Sorghum bicolor (L.) Moench]. Theor Appl Genet 64:325–331
Virupaksha TK, Sastry LVS (1968) Studies on the protein content and amino acid composition of some varieties of grain sorghum. J Agric Food Chem 16:199–203
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Communicated by A. R. Hallauer
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Smith, J.S.C., Smith, O.S. Variation in kafirin and alcohol-soluble glutelin chromatograms of sorghum inbred lines revealed by reversed-phase high-performance liquid chromatography. Theoret. Appl. Genetics 76, 97–107 (1988). https://doi.org/10.1007/BF00288838
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DOI: https://doi.org/10.1007/BF00288838