Skip to main content
Log in

Eyespot resistance gene Pch-1 in H-93 wheat lines. Evidence of linkage to markers of chromosome group 7 and resolution from the endopeptidase locus Ep-D1b

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Gene Pch1, which confers resistance to eyespot disease (Pseudocercosporella herpotrichoides Fron), has been located on chromosome 7D in the H-93 wheat-Aegilops ventricosa transfer lines using isozyme markers and DNA probes corresponding to group 7 chromosomes. Previous experiments had failed to ascertain this location. The lack of segregation of the resistance trait in progeny from reciprocal crosses between lines H-93-70 and VPM1 indicates that their respective resistance factors are allelic. Line H-93-51 carries the endopeptidase allele Ep-D1b but is susceptible to eyespot, which indicates that resistance to eyespot is not a product of the Ep-D locus, as had been proposed in a previous hypohesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Benito C, Gallego FJ, Frade JM, Zaragoza C, Figueiras AM (1990) Chromosomal location of adenylate kinase isozymes in Triticeae species. Theor Appl Genet 79:157–160

    Google Scholar 

  • Delibes A, García-Olmedo F (1973) Biochemical evidence of gene transfer from the Mv genome of Aegilops ventricosa to hexaploid wheat. In: Sears ER, Sears LMS (eds) Proc. 4th Int Wheat Genet Symp. Mo. Agric Exp Stn, University of Missouri, Columbus Mo., pp 161–166

    Google Scholar 

  • Delibes A, Dosba F, Doussinault G, García-Olmedo F, Sanchez-Monge R (1977a) Resistance to eyespot (Cercosporella herpotrichoides) and distribution of biochemical markers in hexaploid lines derived from a double cross (Triticum turgidum x Aegilops ventricosa x T. aestivum. In: Sanchez-Monge E, García-Olmedo F (eds) Interspecific hybridization in plant breeding. Proc 8th Congr EUCARPIA. Polytechnic University, Madrid, pp 91–97

    Google Scholar 

  • Delibes A, Sanchez-Monge R, García-Olmedo F (1977b) Biochemical and cytological studies of genetic transfer from the Mv genome of Aegilops ventricosa into hexaploid wheat. In: Sanchez-Monge E, García-Olmedo F (eds) Interspecific hybridization in plant breeding. Proc 8th Congr EUCARPIA. Polytechnic University, Madrid, pp 81–89

    Google Scholar 

  • Delibes A, Doussinault G, Mena M, Lopez-Braña I, García-Olmedo F (1988) Eyespot resistance gene Pch1 from Aegilops ventricosa is associated with a different chromosome in wheat line H-93–70 than the resistance factor in “Roazon” wheat. Theor Appl Genet 76:573–576

    Google Scholar 

  • Doussinault G, Delibes A, Sanchez-Monge R, García-Olmedo F (1983) Transfer of a dominant gene for resistance to eyespot disease from a wild grass to hexaploid wheat. Nature 303:698–700

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) Technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    CAS  PubMed  Google Scholar 

  • Hart GE, Gale MD (1988) Guidelines for nomenclature of biochemical/molecular loci in wheat and related species. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp. Cambridge, pp 1215–1218

  • Jahier J, Doussinault G, Dosba F, Bourgeois F (1979) Monosomic analysis of resistance to eyespot in the variety ‘Roazon’. In: Ramamujan S Proc 5th Int Wheat Genet Symp Indian Soc Genet Plant Breed, New Delhi, India, pp 437–440

  • Koebner RMD, Miller TE, Snape JW, Law CN (1988) Wheat endopeptidase: genetic control, polymorphism, intrachromosomal gene location, and alien variation. Genome 30:186–192

    Google Scholar 

  • Macer RCF (1966) Resistance to eyespot disease Cercosporella herpotrichoides determined by a seedling test in some forms of Triticum, Aegilops, Secale and Hordeum. J Agric Sci 67:389–397

    Google Scholar 

  • Maraña C, García-Olmedo F, Carbonero P (1988) Linked sucrose synthase genes in group 7 chromosomes in hexaploid wheat (Triticum aestivum L.). Gene 63:253–260

    Google Scholar 

  • Maia V (1967) Obtention de blés tendres résistants au Piétinverse (Cercosporella herpotrichoides) par croisements interspécifiques. CR Acad Agric Fr 53:149–154

    Google Scholar 

  • Mena M (1990) Caracterización de líneas de trigo (Triticum aestivum L.) portadoras de genes de Aegilops ventricosa Tausch, que confieren resistencia a hongos y nematodos. PhD Thesis, Universidad Complutense, Madrid

    Google Scholar 

  • Sharp PJ, Chao S, Desai S, Gale MD (1989) The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theor Appl Genet 78:342–348

    Google Scholar 

  • Taylor B, Powell A (1982) Isolation of plant DNA and RNA. Focus 4:4–6

    Google Scholar 

  • Worland AJ, Law CN, Hollins TW, Koebner RMD, Giura A (1988) Location of a gene for resistance to eyespot (Pseudocercosporella herpotrichoides) on chromosome 7D of bread wheat. Plant Breed 101:43–51

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. Salamini

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mena, M., Doussinault, G., Lopez-Braña, I. et al. Eyespot resistance gene Pch-1 in H-93 wheat lines. Evidence of linkage to markers of chromosome group 7 and resolution from the endopeptidase locus Ep-D1b . Theoret. Appl. Genetics 83, 1044–1047 (1992). https://doi.org/10.1007/BF00232970

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232970

Key words

Navigation