Skip to main content
SpringerLink
Log in

Mapping salt-tolerance genes in tomato (Lycopersicon esculentum) using trait-based marker analysis

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The germination responsiveness of an F2 population derived from the cross Lycopersicon esculentum (UCT5) x L. pennellii (LA716) was evaluated for salt tolerance at two stress levels, 150 mM NaCl + 15 mM CaCl2 and 200 mM NaCl + 20 mM CaCl2. Individuals were selected at both tails of the response distribution. The salt-tolerant and salt-sensitive individuals were genotyped at 16 isozyme loci located on 9 of the 12 tomato chromosomes. In addition, an unselected (control) F2 population was genotyped at the same marker loci, and gene frequencies were estimated in both selected and unselected populations. Trait-based marker analysis was effective in identifying genomic locations (quantitative trait loci, QTLs) affecting salt tolerance in the tomato. Three genomic locations marked by Est-3 on chromosome 1, Prx-7 on chromosome 3, and 6Pgdh-2 and Pgi-1 on chromosome 12 showed significant positive effects, while 2 locations associated with Got-2 on chromosome 7 and Aps-2 on chromosome 8 showed significant negative effects. The identification of genomic locations with both positive and negative effects on this trait suggests the likelihood of recovering transgressive segregants in progeny derived from these parental lines. Similar genomic locations were identified when selection was made either for salt tolerance or salt sensitivity and at both salt-stress treatments. Comparable results were obtained in uni- and bidirectional selection experiments. However, when marker allele gene frequencies in a control population are unknown, bidirectional selection may be more efficient than unidirectional selection in identifying marker-QTL associations. Results from this study are discussed in relationship to the use of molecular markers in developing salt-tolerant tomatoes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bournival BL, Scott JW, Vallejos CE (1989) An isozyme marker for resistance to race 3 Fusarium oxysporum f. sp. lycopersici in tomato. Theor Appl Genet 78:489–494

    Google Scholar 

  • Burr B, Evola E, Burr FA, Beckmann JS (1983) The application of restriction fragment length polymorphism to plant breeding. In: Setlow JK, Hollaender A (eds) Genetic engineering principles and methods, vol 5. Plenum Press, New York, pp 45–59

    Google Scholar 

  • Cook RE (1979) Patterns of juvenile morbidity and recruitment in plants. In: Solbrig OT, Jain S, Johnson GB, Raven PH (eds) Topics in plant population biology. Columbia Univ Press, New York, pp 207–301

    Google Scholar 

  • Dehan K, Tal M (1978) Salt tolerance in the wild relatives of cultivated tomato: Response of Solarium pennellii to high salinity. Irrig Sci 1:71–76

    Google Scholar 

  • Edwards MD, Stuber CW, Wendel JF (1987) Molecular markerfacilitated investigations of quantitative-trait loci in maize. I. Number, genomic distribution and types of gene action. Genetics 116:113–125

    Google Scholar 

  • Epstein E (1972) Mineral nutrition of plants: principles and perspectives. Wiley, New York

    Google Scholar 

  • Falconer DS (1981) Introduction to quantitative genetics, 2nd edn. Longman, New York

    Google Scholar 

  • Foolad MR, Jones RA (1991) Genetic analysis of salt tolerance during germination in Lycopersicon. Theor Appl Genet 81:321–326

    Google Scholar 

  • Foolad MR, Jones RA (1992 a) Models to estimate maternally controlled genetic variation in quantitative seed characters. Theor Appl Genet 83:360–366

    Google Scholar 

  • Foolad MR, Jones RA (1992 b) Parent-offspring regression estimates of heritability for salt tolerance during germination in tomato. Crop Sci 32:439–442

    Google Scholar 

  • Foolad MR, Jones RA, Rodriguez RL (1993) RAPD markers for constructing intraspecific tomato genetic maps. Plant Cell Rep 12:293–297

    Google Scholar 

  • Greenway H, Munns R (1980) Mechanism of salt tolerance in nonhalophytes. Annu Rev Plant Physiol 31:149–190

    Google Scholar 

  • Jones RA (1986 a) The development of salt-tolerant tomatoes: breeding strategies. Acta Hortic 190:101–114

    Google Scholar 

  • Jones RA (1986 b) High salt tolerance potential in Lycopersicon species during germination. Euphytica 35:575–582

    Google Scholar 

  • Jones RA (1987) Genetic advances in salt tolerance. In: Nevins DJ, Jones RA (eds) Tomato biotechnology. Alan R. Liss, New York, pp 125–137

    Google Scholar 

  • Jones RA, Qualset CO (1984) Breeding crops for environmental stress tolerance. In: Collins GB, Petolino JF (eds) Application of genetic engineering to crop improvement. Nijihoff/Junk Publ Dordrecht, pp 305–340

    Google Scholar 

  • Jones RA, Hashim M, El-Beltagy AS (1988) Developmental responsiveness of salt-tolerant and salt-sensitive genotypes of Lycopersicon. In: Whitehead E, Hutchison F, Timmerman B, Varazy R (eds) Arid lands: today and tomorrow. Westview press, Boulder, Colo., pp 765–772

    Google Scholar 

  • Kinzer SM, Schwager SJ, Mutschler MA (1990) Mapping of ripening-related or -specific cDNA clones of tomato (Lycopersicon esculentum). Theor Appl Genet 79:489–496

    Google Scholar 

  • Lande R, Thompson R (1990) Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics 124:743–756

    CAS  PubMed  Google Scholar 

  • Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    CAS  PubMed  Google Scholar 

  • Lebowitz RJ, Soller M, Beckmann JS (1987) Trait-based analyses for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theor Appl Genet 73:556–562

    Google Scholar 

  • Lee E (1980) Statistical methods for survival data analysis. Wadworth, Lifetime Learning Publ, Belmont, Calif.

    Google Scholar 

  • Maas EV (1987) Salt tolerance of plants. In: Christie BR (ed) Handbook of plant science in agriculture, vol 2. CRC Press, Boca Raton, Fla., pp 57–75

    Google Scholar 

  • Martin B, Nienhuis J, King G, Schaefer A (1989) Restriction fragment length polymorphisms associated with water use efficiency in tomato. Science 243:1725–1728

    Google Scholar 

  • Phills BR, Peck NH, McDonald GE, Robinson RW (1979) Differential responses of Lycopersicon and Solanum species to salinity. J Am Soc Hortic Sci 104:349–352

    Google Scholar 

  • Sax K (1923) The association of size differences with seed coat pattern and pigmentation in Phaseolus vulgaris. Genetics 8:552–560

    Google Scholar 

  • Scott SJ, Jones RA (1982) Low temperature seed germination of Lycopersicon species evaluated by survival analysis. Euphytica 31:869–883

    Google Scholar 

  • Shannon MC (1985) Principles and strategies in breeding for higher salt tolerance. Plant Soil 89:227–241

    Google Scholar 

  • Stuber CW, Moll RH, Goodman MM, Schaffer H, Weir BS (1980) Allozyme frequency changes associated with selection for increased grain yield in maize. Genetics 95:225–236

    CAS  Google Scholar 

  • Stuber CW, Edward MD, Wendel JF (1987) Molecular marker-facilitated investigations of quantitative trait loci in maize. II. Factors influencing yield and its component traits. Crop Sci 27:639–648

    Google Scholar 

  • Tal M (1985) Genetics of salt tolerance in higher plants: theoretical and practical considerations. Plant Soil 89:199–226

    Google Scholar 

  • Tal M, Gavish V (1973) Salt tolerance in the wild relatives of the cultivated tomato: Water balance and absicic acid in Lycopersicon esculentum x L. peruvianum under low and high salinity. Aust J Agric Res 24:353–361

    Google Scholar 

  • Tal M, Shannon MC (1983) Salt tolerance in the wild relatives of the cultivated tomato: responses of Lycopersicon esculentum, L. cheesmanii, L. peruvianum, Solanum pennellii and F1 hybrids to high salinity. Aust J Plant Physiol 10:109–117

    Google Scholar 

  • Tanksley SD, Mutschler MM (1990) Linkage map of the tomato (Lycopersicon esculentum) (2n=24). In: O'Brian SJ (ed) Genetic maps; locus maps of complex genomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 6.3–6.15

    Google Scholar 

  • Tanksley SD, Orton TJ (1983) Isozymes in plant genetics and breeding. Elsevier, Amsterdam

    Google Scholar 

  • Tanksley SD, Rick CM (1980) Isozyme gene linkage map of tomato: applications in genetics and breeding. Theor Appl Genet 57:161–170

    Google Scholar 

  • Tanksley SD, Medina-Filho H, Rick CM (1982) Use of naturally-occurring enzyme variation to detect and map genes controlling quantitative traits in an interspecific cross of tomato. Heredity 49:11–25

    Google Scholar 

  • Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: New tools for an old science. Bio/Technology 7:257–264

    Google Scholar 

  • Thoday JM (1961) Location of polygenes. Nature 191:368–370

    Google Scholar 

  • Thompson JN, Thoday JM (1979) Quantitative genetic variation. Academic Press, New York

    Google Scholar 

  • Zamir D, Tal M (1987) Genetic analysis of sodium, potassium and chloride ion content in Lycopersicon. Euphytica 36:187–191

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Vegetable Crops, University of California, Davis, 95616-8746, Davis, CA, USA

    M. R. Foolad & R. A. Jones

Authors
  1. M. R. Foolad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. A. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Foolad, M.R., Jones, R.A. Mapping salt-tolerance genes in tomato (Lycopersicon esculentum) using trait-based marker analysis. Theoret. Appl. Genetics 87, 184–192 (1993). https://doi.org/10.1007/BF00223763

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00223763

Key words

Search

Navigation