Abstract
RFLPs were used to investigate components of host-plant response to Exserohilum turcicum in 150 unselected F2∶3 lines of a B52/Mo17 maize population. Following inoculation with spore suspensions of the pathogen (race 0), components of disease development were measured and then quantitative trait mapping was performed to identify the location and effects of quantitative trait loci (QTLs) determining host-plant response. Components of interest were the average number of lesions per leaf, the average percent leaf tissue diseased (severity) and the average size of lesions (cm2). Based on a LOD threshold of 2.31 (P<0.05), the number of lesions appears to be associated with QTLs on chromosomes 1S, 3L, 5S. Severity was associated with analogous regions and, in addition, QTLs on chromosomes 7L and 8L. Most QTLs, for either of these two components, involve additive gene action and partial dominance or overdominance. In contrast, lesion size was associated with QTLs on chromosomes 7L and 5L; recessive gene action may be involved at 7L.
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References
Agrios GN (1988) Plant pathology, 3rd edn. Academic Press, San Diego, California
Bentolila S, Guitton C, Bouvet N, Sailland A, Nykaza S, Freyssinet G, (1991) Identification of an RFLP marker tightly linked to the Ht1 gene in maize. Theor Appl Genet 82:393–398
Brewster VA, Carson ML, Wicks, III Z (1992) Mapping components of partial resistance to northern leaf blight of maize using reciprocal translocations. Phytopathology 82:225–229
Bubeck DM, Goodman MM, Beavis WD, Grant D (1993) Quantitative trait loci controlling resistance to gray leaf spot in maize. 33:838–847
Dodd JL (1980) The role of plant stresses in development of corn stalk rots. Plant Dis 64:533–537
Doebley J, Stec A, Wendel J, Edwards M (1990) Genetic and morphological analysis of a maize-teosinte F2 population: implications for the origin of maize. Proc Natl Acad Sci USA 87:9888–9892
Edwards MD, Stuber CW, Wendel JF (1987) Molecular-marker-facilitated investigations of quantitative-trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics 116:113–125
Freymark PJ, Lee M, Martinson C, Woodman WL (1993) Quantitative and qualitative trait loci affecting host-plant response to Exserohilum turcicum in Maize (Zea mays L.). Theor Appl Genet 87:537–544
Gevers HO (1975) A new major gene for resistance to Helminthosporium turcicum leaf blight of maize. Plant Dis Reptr 59:296–299
Hallauer AR, Miranda JB (1988) Quantitative genetics in maize breeding, 2nd edn. Iowa State University Press, Ames, Iowa
Hilu HM, Hooker AL (1964) Host-pathogen relationship of Helminthosporium turcicum in resistant and susceptible corn seedlings. Phytopathology 54:570–575
Hooker AL (1963) Inheritance of chlorotic-lesion resistance to Helminthosporium turcicum in seedling corn. Phytopathology 53:660–662
Hooker AL (1975) Helminthosporium turcicum as a pathogen of corn. Rep Tottori Mycol Inst (Jpn) 12:115–125
Hooker AL (1977) A second major gene locus in corn for Chlorotic-Lesion resistance to Helminthosporium turcicum. Crop Sci 17:132–135
Hooker AL, Kim SK (1973) Monogenic and multigenic resistance to Helminthosporium turcicum in corn. Plant Dis Reptr 57:586–589
Hughes GR, Hooker AL (1971) Gene action conditioning resistance to northern leaf blight in maize. Crop Sci 11:180–184
Jenkins MT, Robert AL (1961) Further genetic studies of resistance to Helminthosporium turcicum Pass. by means of chromosomal translocations. Crop Sci 1:450–455
Jenkins MT, Robert AL, Findley WR, Jr (1957) Genetic studies of resistance to Helminthosporium turcicum in maize by means of chromosomal translocations. Agron J 49:197–201
Knapp SJ, Stroup WW, Ross WM (1985) Exact confidence intervals for heritability on a progeny mean basis. Crop Sci 25:192–194
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Lim SM, Kinsey JG, Hooker AL (1974) Inheritance of virulence in Helminthosporium turcicum to monogenic resistant corn. Phytopathology 64:1150–1151
Lincoln SE, Lander ES (1990) Mapping genes controlling quantitative traits using MAPMAKER/QTL. Whitehead Institute for Biomedical Research, Cambridge, Massachusetts
McMullen MD, Louie R (1989) The linkage of molecular markers to a gene controlling the symptom response in maize to maize dwarf mosaic virus. Mol Plant-Microbe Interact 2:309–314
Mode CJ, Robinson HF (1959) Pleiotropism and the genetic variance and covariance. Biometrics 15:518–537
Mohan Ram HY, Nayyar VL (1978) A leaf-clearing technique with a wide range of applications. Proc Indian Acad Sci 87:125–127
Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley D (1988) Resolution of quantitative traits into mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726
Perkins JM, Pedersen WL (1987) Disease development and yield losses associated with northern leaf blight on corn. Plant Dis 71:940–943
Shapiro SS, Wilk MB (1965) An analysis of variance for normality (complete samples). Biometrika 52:591–611
Simone GW (1978) Inheritance of resistance in fifteen corn selections to Helminthosporium turcicum. PhD dissertation, University of Illinois, Urbana. (Diss Abstr 78–11293)
Smith DR, White DG (1988) Diseases of corn. In: Sprague GF, Dudley JW (eds) Corn and corn improvement, 3rd edn. American Society of Agronomy, Madison, Wisconsin, pp 700–766
Stuber CW, Edwards MD, Wendel JF (1987) Molecular markerfacilitated investigations of quantitative trait loci in maize. II.Factors influencing yield and its component traits. Crop Sci 27:639–648
Ullstrup AJ (1977) Diseases of corn. In: Sprague GF (ed) Corn and Corn Improvement, 2nd edn. Am Soc Agron, Madison, Wisconsin, pp 391–500
Zaitlin D, DeMars SJ, Gupta M (1992) Linkage of a second gene for NCLB resistance to molecular markers in maize. Maize Genet Coop Newslett 66:69–70
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Communicated by F. Salamini
Journal Paper No. J-15178 of the Iowa Agriculture and Home Economic Experiment Station, Ames, Iowa. Project No. 3134
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Freymark, P.J., Lee, M., Martinson, C.A. et al. Molecular-marker-facilitated investigation of host-plant response to Exserohilum turcicum in maize (Zea mays L.): components of resistance. Theoret. Appl. Genetics 88, 305–313 (1994). https://doi.org/10.1007/BF00223637
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DOI: https://doi.org/10.1007/BF00223637