Abstract
Broadening of the genetic base and systematic exploitation of heterosis in cultivated lentils requires reliable information on genetic diversity in the germplasm. The ability of random amplified polymorphic DNA (RAPD) to distinguish among different taxa of Lens was evaluated for several geographically dispersed accessions/cultivars of four diploid Lens species. This study was carried out to assess whether RAPD data can provide additional evidence about the origin of the cultivated lentil and to measure genetic variability in lentil germplasm. Three cultivars of Lens culinaris ssp. culinaris, including one microsperma, and two macrosperma types, and four wild species (L. culinaris ssp. orientalis, L. odemensis and L. nigricans) were evaluated for genetic variability using a set of 1 11-mer and 14 random 10-mer primers. One hundred and fifty-eight reproducible and scorable DNA bands were observed from these primers. Genetic distances between each of the accessions were calculated from simple matching coefficients. Split decomposition analysis of the RAPD data allowed construction of an unrooted tree. This study revealed that (1) the level of intraspecific genetic variation in cultivated lentils is narrower than that in some wild species. (2) L. culinaris ssp. orientalis is the most likely candidate as a progenitor of the cultivated species, (3) L. nigricans accession W6 3222 (unknown) and L. c. ssp. orientalis W6 3244 (Turkey) can be reclassified as species of L. odemensis and (4) transmission of genetic material in Lens interspecific hybrids is genotypically specific, as identified by the RAPD markers in our study.
Similar content being viewed by others
References
Abbo S, Ladizinsky G, Weeden NF (1992) Genetic analysis and linkage studies of seed weight in lentil. Euphytica 58:259–266
Abo-elwafa A, Murai K, Shimada T (1995) Intra- and inter-specific variation in Lens revealed by RAPD markers. Theor Appl Genet 90:335–340
Ahmad M, Fautrier AG, Burritt DJ (1995a) Genetic diversity and relationships in Lens species and their interspecific hybrids as determined by SDS-PAGE. Plant Breed (in press)
Ahmad M, Fautrier AG, McNeil DL, Burritt DJ, Hill GD (1995b) Attempts to overcome postfertilization barrier in interspecific hybrids of the genus Lens. Plant Breed 114:558–560
Ahmad M, McNeil DL, Fautrier AG, Burritt DJ (1995c) Use of RAPD's and seed proteins for determining relationships between lentil species. In: Kriedemann P (ed) Proc 35th Annu Gen Meet NZ Soc Plant Physiol 17th Annu Gen Meet. Australian Society of Plant Physiologists Inc. Publishers, Sydney p 19
Appels R, Lagudah ES (1990) Notes for molecular cytogenetics/ RFLP course. CSIRO, Australia
Bandelt HJ, Dress AWM (1992) Split decomposition: a new and useful approach to phylogenetic analysis of distance data. Mol Phylogenet Evol 1:242–252
Barulina H (1930) Lentils of the USSR and of other countries. Bull Appl Bot Plant Breed [Suppl]40:1–319
Castiglione S, Wang G, Damiani G, Bandi C, Bisoffi S, Sala F (1993) RAPD fingerprintts for identification and for taxonomic studies of elite poplar (Populus ssp.) clones. Theor Appl Genet 87:54–59
Ford R, Pang E, Taylor P (1995) Developments of molecular markers for the genetic improvement of lentiles. In: Kriedemann P (ed) Proc 35th Annu Gen Meet NZ Soc Plant Physiol 17th Annu Gen Meet. Australian Society of Plant Physiologists Inc. Publishers, Sydney p 18
GENSTAT (1989) GENSTAT 5 reference manual. Oxford University Press, Oxford
Godshalk EB, Lee M, Lampkey KR (1990) Relationship of restriction fragment length polymorphisms to single-cross hybrid performance in maize. Theor Appl Genet 80:273–280
Gonzalez JM, Ferrer E (1993) Random amplified polymorphic DNA analysis in Hordeum species. Genome 86:497–504
Havey MJ, Muehlbauer FJ (1989) Variability for restriction fragment lengths and phylogenesis in lentil. Theor Appl Genet 77:839–843
Hoffman DL, Solits DE, Muehlbauer FJ, Ladizinsky G (1986) Isozyme polymorphism in Lens (Leguminosae). Syst Bot 11:392–402
Jain A, Bhatia S, Banga SS, Prakash S, Lakshmikumaran M (1994) Potential use of random amplified polymorphic DNA (RAPD) technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theor Appl Genet 88:116–122
Kresovich S, Williams JGK, McFerson JR, Routman EJ, Schael BA (1992) Characterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay. Theor Appl Genet 85:190–196
Ladizinsky G (1979a) The origin of lentil and its wild genepool. Euphytica 28:179–187
Ladizinsky G (1979b) Species relationships in the genus Lens as indicated by seed-protein electrophoresis. Bot Gaz 140:449–451
Ladizinsky G (1993) Wild lentil. Crit Rev Plant Sci 12:169–184
Landizinsky G, Braun D, Goshen D, Muelbauer FJ (1984) The biological species of the genus Lens L. Bot Gaz 145:253–261
Lanham PG, Brennan RM, Hackett C, McNeil RJ (1995) RAPD fingerprinting of blackcurrent (Ribes nigrum L.) cultivars. Theor Appl Genet 90:166–172
Mayer MS, Soltis PS (1994) Chloroplast DNA phylogeny of Lens (Leguminosae): origin and diversity of the cultivated lentil. Theor Appl Genet 87:773–781
Melchinger AE, Lee M, Lamkey KR, Hallauer AR, Woodman WL (1990) Genetic diversity for restriction fragment length polymorphisms: relation to estimated genetic effects in maize inbres. Crop Sci 30:1033–1040
Moll RH, Lonnquist JH, Fortuna JV, Johnson CE (1965) The relationship of heterosis and genetic divergence in maize. Genetics 52:139–144
Muench DG, Slinkard AE, Scoles GJ (1991) Determination of genetic variation and taxonomy in lentil (Lens Miller) species by chloroplast DNA polymorphism. Euphytica 56:213–218
Nei M, Li W (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5256–5273
Nienhuis J, Slocum MK, Devos DA, Muren R (1992) Genetic similarity among Brassica oleracea genotypes as measured by restriction fragment length polymorphisms. J Am Soc Hortic Sci 118:298–303
Pinkas R, Zamir D, Ladizinsky G (1985) Allozyme divergence and evolution in the genus Lens. Plant Syst Evol 151:131–140
Rajora OP, Mahon JD (1994) Inheritance of mitochondrial DNA in lentil (Lens culinaris Medik.). Theor Appl Genet 89:206–210
Rajora OP, Mahon JD (1995) Paternal plastid DNA can be inherited in lentil. Theor Appl Genet 90:607–610
Sammour RH (1994) Species relationships in genus Lens as indicated by elecrophoresis — a reappraisal. Lens Newsl 21:1–4
Smith OS, Smith JSC, Bowen SL, Tenborg RA, Wall SJ (1990) Similarities among a group of elite maize inbreds as measured by pedigree, F1 grain yield, grain yield, heterosis, and RFLPs. Theor Appl Genet 80:833–840
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Wilkie SE, Isaac PG, Slater RJ (1993) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theor Appl Genet 86:497–504
Williams CE, Clair DAS (1993) Phenetic relationships and levels of variability detected by restriction fragment length polymorphisms and random amplified polymorphic DNA analysis of cultivated and wild accessions of Lycopersicon esculentum. Genome 36:619–630
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Williams JT, Sanchez AMC, Jackson MT (1974) Studies on lentils and their variation. 1. The taxonomy of the species. SABRAO J 6:133–145
Author information
Authors and Affiliations
Additional information
Communicated by J. Mac Key
Rights and permissions
About this article
Cite this article
Ahmad, M., McNeil, D.L., Fautrier, A.G. et al. Genetic relationships in Lens species and parentage determination of their interspecific hybrids using RAPD markers. Theoret. Appl. Genetics 92, 1091–1098 (1996). https://doi.org/10.1007/BF00224054
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224054