Abstract
Twenty-five partial amphiploids (2n=8x=56), which were derived from hybrids of wheat (Triticum aestivum L.) with either Thinopyrum ponticum (Podpera) Liu & Wang, Th. intermedium (Host) Barkworth & D. Dewey, or Th. junceum (L.) A. Löve, were assayed for resistance to BYDV serotype PAV by slot-blot hybridization with viral cDNA of a partial coat protein gene. Three immune lines were found among seven partial amphiploids involving Th. ponticum. Seven highly resistant lines were found in ten partial amphiploids involving Th. intermedium. None of eight partial amphiploids or 13 addition lines of Chinese Spring — Th. junceum were resistant to BYDV. Genomic in situ hybridization demonstrated that all of the resistant partial amphiploids, except TAF46, carried an alien genome most closely related to St, whether it was derived from Th. ponticum or Th. intermedium. The two partial amphiploids carrying an intact E genome of Th. ponticum are very susceptible to BYDV-PAV. In TAF46, which contains three pairs of St- and four pairs of E-genome chromo somes, the gene for BYDV resistance has been located to a modified 7 St chromosome in the addition line L1. This indicates that BYDV resistance in perennial polyploid parents, i.e., Th. ponticum and Th. intermedium, of these partial amphiploids is probably controlled by a gene(s) located on the St-genome chromosome(s).
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References
Banks PM, Larkin PJ (1995) Registration of three BYDV-resistant wheat germ plasms: TC5, TC6, and TC9. Crop Sci 35:599–600
Banks PM, Xu SJ, Wang RRC, Larkin PJ (1993) Varying chromosome composition of 56-chromosome wheat x Thinopyrum intermedium partial amphiploids. Genome 36:207–215
Banks PM, Larkin PJ, Bariana HS, Lagudah ES, Appels R, Waterhouse PM, Brettell RIS, Chen X, Xu HJ, Xin ZY, Qian YT, Zhou XM, Cheng ZM, Zhou GH (1995) The use of cell culture for subchromosomal introgressions of barley yellow dwarf virus resistance from Thinopyrum intermedium to wheat. Genome 38:395–405
Brettle RIS, Banks PM, Cauderon Y, Chen X, Cheng ZM, Larkin PJ, Waterhouse PM (1988) A single wheatgrass chromosome reduces the concentration of barley yellow dwarf virus in wheat. Ann Appl Biol 113:599–603
Burnett PA (1990) World perspectives on barley yellow dwarf. CIMMYT, Mexico, D.F. Mexico
Cauderon Y, Saigne B, Dauge M (1973) The resistance to wheat rusts of Agropyron intermedium and its use in wheat improvement. Proc 4th Int Wheat Genet Symp, pp 401–407
Chen CF, Amstrong K (1994) Genomic in situ hybridization in Avena sativa. Genome 37:607–612
Cisar G, Brown CM, Jedlinski H (1982) Effect of fall or spring infection and sources of tolerance of barley yellow dwarf of winter wheat. Crop Sci 22:474–478
Comeau A, St-Pierre CA, Collin J (1993) Interspecific hybrids of wheat as sources of barley yellow dwarf virus resistance. In: Damania A (ed) Biodiversity and wheat improvement. John Wiley and Sons, Chichester, UK, pp 83–94
Dvorak J (1971) Hybrids between diploid Agropyron elongatum and Aegilops squarrosa. Can J Genet Cytol 13:90–94
Dvorak J (1980) Homoeology between Agropyron elongatum chromosomes and Triticum aestivum chromosomes. Can J Genet Cytol 22:237–259
Friebe B, Mukai Y, Gill BS, Cauderon Y (1992) C-banding and in situ hybridization analyses of Agropyron intermedium, a partial wheat x Ag. intermedium amphiploid, and six derived chromosome addition lines. Theor Appl Genet 83:589–596
Hsiao C, Wang RRC, Dewey DR (1986) Karyotype analysis and genome relationships of 22 diploid species in the tribe Triticeae. Can J Genet Cytol 28:109–120
Hsiao C, Chatterton NJ, Asay KH, Jensen KB (1994) Phylogenetic relationships of the monogenomic species of the wheat tribe, Triticeae (Poaceae), inferred from nuclear rDNA (ITS) sequences. Genome 38:211–223
Joppa LR (1987) Aneuploid analysis in tetraploid wheat. In: Heyne EG (ed) Wheat and wheat improvement (2nd edn.), pp 255–267
Kim N-S, Armstrong KC, Fedak G, Fominaya A, Whelan EWP (1993) Cytogenetical and molecular characterization of a chromosome interchange and addition lines in Cadet involving chromosome 5B of wheat and 6Ag of Lophopyrum ponticum. Theor Appl Genet 86:827–832
Knott DR (1989) The wheat rust-breeding for resistance. Springer, Berlin Heidelberg New York
Li ZS, Rong S, Chen S, Zhong GC, Mu SM (1985) Wheat wide hybridization. Published by Scientific Publishing Company, Beijing
Lister RM, Barbara DJ, Kawata EE, Ueng PP, Fattouh F, Larkins BA (1990) Development and use of cDNA probes in studies of barley yellow dwarf virus. In: Burnett PA (ed) World perspectives on barley yellow dwarf. CIMMYT, Mexico, D.F. Mexico, pp 186–193
Liu ZW, Wang RRC (1993) Genome analysis of Elytrigia caespitosa, Lophopyrum nodosum, Pseudoroegneria geneiculata ssp. scythica and Thinopyrum intermedium (Triticeae Gramineae). Genome 36:102–111
Mujeeb-Kazi (1994) Use of annual and perennial Triticeae species for wheat improvement. In: Wang RRC, Jensen KB, Jaussi C (eds) Proc 2nd Int Triticeae Symp, Logan, Utah, USA, pp 86–89
Plourde A, Comeau A, St-Pierre CA (1992) Barley yellow dwarf virus resistance in Triticum aestivum x Leymus angustus hybrids. Plant Breed 108:97–103
Robertson NL, French R, Gray SM (1991) Use of grop-specific primers and the polymerase chain reaction for the detection and identification of luteoviruses. J Gen Virol 72:1473–1477
Sharma H, Ohm H, Goulart L, Lister R, Appels R, Benlhabib O (1995) Introgression and characterization of barley yellow dwarf virus resistance from Thinopyrum intermedium into wheat. Genome 38:406–413
Sun SC (1981) The approach and methods of breeding new varieties and new species from Agrotriticum hybrids. Acta Agron Sinica 7:51–58
Wang RRC (1992) Genome relationships in the perennial Triticeae based on diploid hybrids and beyond. Hereditas 116:133–136
Xin ZY, Brettell RIS, Cheng ZM, Waterhouse PM, Appels R, Banks PM, Zhou GH, Chen X, Larkin PJ (1988) Characterization of a potential source of barley yellow dwarf virus resistance for wheat. Genome 30:250–257
Xin ZY, Xu HJ, Chen X, Lin ZS, Zhou GH, Qian YT, Cheng ZM, Larkin PJ, Banks P, Appels R, Glarke B, Brettell RIS (1991) Development of a common wheat germ plasm-resistant to barley yellow dwarf virus by biotechnology. Sci China (Series B) 34:1055–1062
Xu SJ, Banks PM, Dong YS, Zhou RH, Larkin PJ (1994) Evaluation of Chinese Triticeae for resistance to barley yellow dwarf virus. Genet Res Crop Evol 41:35–41
Zhang XY, Dong YS (1994) Cytogenetic research on a hybrid of Triticum with Thinopyrum ponticum as well as its derivatives. II. Comparative research of eleven partial amphiploids derived from hybrid offspring of T. aestivum with both Th. ponticum and Th. intermedium. Acta Genet Sinica 21:287–296
Zhang XY, Dong YS, Wang RRC (1996) Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum x Thinopyrum ponticum by in situ hybridization, isozyme analysis, and RAPD. Genome 39:1062–1071
Zhong GY, McGuire PE, Qualset CO, Dvorak J (1994) Cytological and molecular characterization of a Triticum aestivum x Lyphopyrum ponticum backcross derivative resistance to barley yellow dwarf. Genome 37:876–887
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Zhang, X.Y., Koul, A., Petroski, R. et al. Molecular verification and characterization of BYDV-resistant germ plasms derived from hybrids of wheat with Thinopyrum ponticum and Th. intermedium . Theoret. Appl. Genetics 93, 1033–1039 (1996). https://doi.org/10.1007/BF00230121
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DOI: https://doi.org/10.1007/BF00230121