Summary
Albumin has been found to be more effective than gelatine in preventing insulin loss from insulin containing solutions. It is probable that this has led to falsely elevated levels of insulin-like activity (ILA) reported with bio-assays. Preparations of crystalline human albumin have been found to be free from ILA and from insulin measured by the radio-immuno-assay. Other human albumin prepared by acid-ethanol extraction (that has been shown previously to contain insulin antagonistic properties on the isolated rat hemidiaphragm) has contained significant ILA on the isolated rat fat pad and also contained insulin demonstrable by radio-immuno-assay.
Résumé
Il a été démontré que l'albumine peut prévenir mieux que la gélatine la perte d'insuline d'une solution contenant de l'insuline. Ceci a probablement conduit à des rapports erronés de taux d'ILA élevés dans les dosages avec des méthodes biologiques. Des préparations d'albumine humaine cristalline ne montraient ni d'ILA, ni d'insuline décelable par la méthode radio-immunologique. L'albumine humaine préparée après extraction par l'alcool-acide (qui dans des expériences antérieures a démontré des propriétés anti-insuliniques avec l'hémidiaphragme isolé du rat) montre une ILA considérable avec le tissu adipeux isolé du rat et aussi de l'insuline qui peut être démontrée par la méthode radioimmunologique.
Zusammenfassung
Es konnte gezeigt werden, daß Albumin stärker als Gelatine den Insulinverlust insulin-haltiger Lösungen verhindern kann. Das hat wahrscheinlich zu falsch erhöhten Spiegeln der insulinähnlichen Aktivität (ILA) geführt, über die bei Bestimmung mit biologischen Methoden berichtet wurde. Präparate kristallinen menschlichen Albumins zeigten keine ILA und auch kein mit der radioimmunologischen Methode bestimmbares Insulin. Menschliches durch Säurealkohol-extraktion gewonnenes Albumin (das nach früheren Untersuchungen insulinantagonistische Eigenschaften am isolierten Rattenhemidiaphragma hat) zeigte am isolierten Rattenfettgewebe eine deutliche ILA und auch mit der radioimmunologischen Methode bestimmbares Insulin.
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This work was carried out while one of us (P.H.S.) was working as a Leverhulme Research Fellow and (A. R.) was supported by a grant from the British Diabetic Association.
We are grateful to Dr. W.Maycock of the Lister Institute for a supply of highly purified Human Albumin.
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Sönksen, P.H., Ellis, J.P., Lowy, C. et al. A quantitative evaluation of the relative efficiency of gelatine and albumin in preventing insulin adsorption to glass. Diabetologia 1, 208–210 (1966). https://doi.org/10.1007/BF01257913
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DOI: https://doi.org/10.1007/BF01257913