Summary
The binding of125I-labelled insulin to human adipocytes was studied at 37° C. The precipitability of the125I-labelled insulin preparation (0.03 nmol/l) in trichloroacetic acid and the concentration of biologically active insulin (7.5 nmol/l) remained constant in buffer incubated with human adipocytes (100 μl cells/ml suspension) for 30–60 minutes at 37° C, whereas more than half of the insulin was inactivated by rat fat cells under the same conditions. A constant level of binding of125I-labelled insulin (0.03 nmol/l) to human adipocytes was obtained after 45 minutes. The apparent dissociation constant of receptor binding was about 0.2 nmol/l as compared to about 2 nmol/l for rat adipocytes. Conversion of [U-14C]glucose to lipids was stimulated half-maximally by about 0.05 nmol/l of insulin (similar to rat adipocytes). Thus, half-maximal stimulation of human adipocytes was obtained with a receptor occupancy of about 20–30 per cent.
Article PDF
Similar content being viewed by others
References
Olefsky, J.M., Jen, P., Reaven, G.M.: Insulin binding to isolated human adipocytes. Diabetes23, 565–571 (1974)
Amatruda, J.M., Livingston, J.N., Lockwood, D.H.: Insulin receptor: Role in the resistance of human obesity to insulin. Science188, 264–266 (1975)
Gliemann, J., Østerlind, K., Vinten, J., Gammeltoft, S.: A procedure for measurement of distribution spaces in isolated fat cells. Biochim. Biophys. Acta268, 1–9 (1972)
Olefsky, J.M.: Decreased insulin binding to adipocytes and circulating monocytes from obese subjects. J. Clin. Invest57, 1165–1172 (1976)
Goldrick, R.B.: Effects of insulin on glucose metabolism in isolated human fat cells. J. Lipid Res.8, 581–588 (1967)
Gliemann, J., Vinten, J.: Lipogenesis and insulin sensitivity of single fat cells. J. Physiol.236, 499–516 (1974)
Gammeltoft, S., Gliemann, J.: Binding and degradation of125I-labelled insulin by isolated rat fat cells. Biochim. Biophys. Acta320, 16–32 (1973)
Gliemann, J.: Assay of insulin-like activity by the isolated fat cell method I. Factors influencing the response to crystalline insulin. Diabetologia3, 382–388 (1967)
Moody, A.J., Stan, M.A., Stan, M., Gliemann, J.: A simple free fat cell bioassay for insulin. Horm. Metab. Res.6, 12–16 (1974)
Terris, S., Steiner, D.F.: Binding and degradation of125I-insulin by rat hepatocytes. J. Biol. Chem.250, 8389–8398 (1975)
Gliemann, J.: Two groups of insulin receptors in rat adipocytes, one of them related to insulin degradation. (abstract) Diabetologia12, 393 (1976)
Harrison, L.C., Martin, F.I.R., Melick, R.A.: Correlation between insulin receptor binding in isolated fat cells and insulin sensitivity in obese human subjects. J. Clin. Invest58, 1435–1441 (1976)
Katz, J., Rognstad, R.: The metabolism of tritiated glucose by rat adipose tissue. J. Biol. Chem.241, 3600–3610 (1966)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Andersen, O., Gliemann, J. & Gammeltoft, S. Receptor binding and biological effect of insulin in human adipocytes. Diabetologia 13, 589–593 (1977). https://doi.org/10.1007/BF01236312
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01236312