Summary
Using ferritin-labelledRicinus communis agglutinin to detect lectin-binding sites of the pancreatic B-cell surface, we show that limited regions of the plasma membrane are deprived of lectin-binding sites over marginated secretory granules. Such deprived regions increased during glucose stimulation of B-cells in monolayer culture: 56±8 of them were found in high (300 mg/100 ml) glucose as compared to only 27±5 in low (50 mg/100ml) glucose (p<0.005). In addition, non-membrane, intracytoplasmic bridges were detected between the plasma membrane and the membrane of the marginated granule suggesting the involvement of cell web components in promoting the change in surface labelling.
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Orci, L., Amherdt, M., Roth, J. et al. Inhomogeneity of surface labelling of B-cells at prospective sites of exocytosis. Diabetologia 16, 135–138 (1979). https://doi.org/10.1007/BF01225464
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DOI: https://doi.org/10.1007/BF01225464